Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2586 and Dshi_2587 are separated by 289 nucleotides Dshi_2587 and Dshi_2588 overlap by 4 nucleotides Dshi_2588 and Dshi_2589 overlap by 4 nucleotides
Dshi_2586: Dshi_2586 - HpcH/HpaI aldolase (RefSeq), at 2,751,482 to 2,752,237
_2586
Dshi_2587: Dshi_2587 - DNA damage response exonuclease, associated with a ligase (from data) , at 2,752,527 to 2,753,555
_2587
Dshi_2588: Dshi_2588 - Alpha/beta hydrolase fold-3 domain protein (RefSeq), at 2,753,552 to 2,754,448
_2588
Dshi_2589: Dshi_2589 - ATP dependent DNA ligase (RefSeq), at 2,754,445 to 2,756,049
_2589
Position (kb)
2752
2753
2754 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2751.629 kb on + strand, within Dshi_2586 at 2751.648 kb on + strand, within Dshi_2586 at 2751.833 kb on + strand, within Dshi_2586 at 2752.255 kb on - strand at 2752.350 kb on - strand at 2752.444 kb on + strand at 2752.444 kb on + strand at 2752.452 kb on - strand at 2752.452 kb on - strand at 2752.510 kb on + strand at 2752.620 kb on + strand at 2752.639 kb on - strand, within Dshi_2587 at 2752.641 kb on + strand, within Dshi_2587 at 2752.649 kb on - strand, within Dshi_2587 at 2752.654 kb on - strand, within Dshi_2587 at 2752.751 kb on - strand, within Dshi_2587 at 2752.797 kb on + strand at 2752.859 kb on + strand, within Dshi_2587 at 2752.892 kb on + strand, within Dshi_2587 at 2752.892 kb on + strand, within Dshi_2587 at 2752.892 kb on + strand, within Dshi_2587 at 2752.892 kb on + strand, within Dshi_2587 at 2753.059 kb on - strand, within Dshi_2587 at 2753.086 kb on - strand, within Dshi_2587 at 2753.131 kb on + strand, within Dshi_2587 at 2753.131 kb on + strand, within Dshi_2587 at 2753.154 kb on - strand, within Dshi_2587 at 2753.170 kb on - strand, within Dshi_2587 at 2753.180 kb on - strand, within Dshi_2587 at 2753.192 kb on - strand, within Dshi_2587 at 2753.202 kb on + strand, within Dshi_2587 at 2753.232 kb on + strand, within Dshi_2587 at 2753.262 kb on - strand, within Dshi_2587 at 2753.362 kb on + strand, within Dshi_2587 at 2753.367 kb on + strand, within Dshi_2587 at 2753.432 kb on - strand, within Dshi_2587 at 2753.477 kb on + strand at 2753.542 kb on - strand at 2753.624 kb on - strand at 2753.903 kb on + strand, within Dshi_2588 at 2754.035 kb on - strand, within Dshi_2588 at 2754.035 kb on - strand, within Dshi_2588 at 2754.172 kb on - strand, within Dshi_2588 at 2754.206 kb on - strand, within Dshi_2588 at 2754.365 kb on - strand at 2754.370 kb on + strand at 2754.370 kb on + strand at 2754.380 kb on + strand at 2754.428 kb on - strand at 2754.463 kb on + strand at 2754.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 2,751,629 + Dshi_2586 0.19 -0.1 2,751,648 + Dshi_2586 0.22 +0.8 2,751,833 + Dshi_2586 0.46 -2.1 2,752,255 - -3.1 2,752,350 - +0.2 2,752,444 + -1.6 2,752,444 + +0.2 2,752,452 - +0.2 2,752,452 - +1.2 2,752,510 + +0.2 2,752,620 + -0.1 2,752,639 - Dshi_2587 0.11 +0.2 2,752,641 + Dshi_2587 0.11 +1.2 2,752,649 - Dshi_2587 0.12 -3.4 2,752,654 - Dshi_2587 0.12 +0.2 2,752,751 - Dshi_2587 0.22 -0.8 2,752,797 + +0.2 2,752,859 + Dshi_2587 0.32 -2.7 2,752,892 + Dshi_2587 0.35 +1.2 2,752,892 + Dshi_2587 0.35 +0.2 2,752,892 + Dshi_2587 0.35 +1.8 2,752,892 + Dshi_2587 0.35 -2.6 2,753,059 - Dshi_2587 0.52 +2.2 2,753,086 - Dshi_2587 0.54 -2.8 2,753,131 + Dshi_2587 0.59 -1.2 2,753,131 + Dshi_2587 0.59 -0.8 2,753,154 - Dshi_2587 0.61 -0.8 2,753,170 - Dshi_2587 0.62 -0.7 2,753,180 - Dshi_2587 0.63 -1.2 2,753,192 - Dshi_2587 0.65 +1.2 2,753,202 + Dshi_2587 0.66 -1.1 2,753,232 + Dshi_2587 0.69 +0.2 2,753,262 - Dshi_2587 0.71 -1.3 2,753,362 + Dshi_2587 0.81 -3.3 2,753,367 + Dshi_2587 0.82 +2.2 2,753,432 - Dshi_2587 0.88 +1.8 2,753,477 + -1.1 2,753,542 - +0.2 2,753,624 - +0.2 2,753,903 + Dshi_2588 0.39 -2.6 2,754,035 - Dshi_2588 0.54 -1.5 2,754,035 - Dshi_2588 0.54 +1.5 2,754,172 - Dshi_2588 0.69 -1.4 2,754,206 - Dshi_2588 0.73 +0.2 2,754,365 - +0.2 2,754,370 + -0.8 2,754,370 + +0.9 2,754,380 + -0.3 2,754,428 - +0.4 2,754,463 + -1.8 2,754,481 - -1.7
Or see this region's nucleotide sequence