Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2444 and Dshi_2445 overlap by 1 nucleotides Dshi_2445 and Dshi_2446 are separated by 1 nucleotides Dshi_2446 and Dshi_2447 overlap by 4 nucleotides
Dshi_2444: Dshi_2444 - 4-hydroxythreonine-4-phosphate dehydrogenase (RefSeq), at 2,587,296 to 2,588,300
_2444
Dshi_2445: Dshi_2445 - transcriptional regulator, GntR family (RefSeq), at 2,588,300 to 2,589,013
_2445
Dshi_2446: Dshi_2446 - dihydrodipicolinate synthetase (RefSeq), at 2,589,015 to 2,589,926
_2446
Dshi_2447: Dshi_2447 - Mandelate racemase/muconate lactonizing protein (RefSeq), at 2,589,923 to 2,591,098
_2447
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2587.330 kb on + strand at 2587.453 kb on + strand, within Dshi_2444 at 2587.648 kb on + strand, within Dshi_2444 at 2587.650 kb on - strand, within Dshi_2444 at 2587.656 kb on + strand, within Dshi_2444 at 2587.827 kb on - strand, within Dshi_2444 at 2587.873 kb on - strand, within Dshi_2444 at 2588.002 kb on - strand, within Dshi_2444 at 2588.085 kb on + strand, within Dshi_2444 at 2588.125 kb on - strand, within Dshi_2444 at 2588.125 kb on - strand, within Dshi_2444 at 2588.259 kb on + strand at 2588.259 kb on - strand at 2588.260 kb on + strand at 2588.369 kb on - strand at 2588.410 kb on - strand, within Dshi_2445 at 2588.427 kb on - strand, within Dshi_2445 at 2588.583 kb on - strand, within Dshi_2445 at 2588.674 kb on + strand, within Dshi_2445 at 2588.682 kb on - strand, within Dshi_2445 at 2588.689 kb on + strand, within Dshi_2445 at 2588.764 kb on + strand, within Dshi_2445 at 2588.874 kb on - strand, within Dshi_2445 at 2588.961 kb on + strand at 2588.994 kb on - strand at 2589.011 kb on - strand at 2589.040 kb on - strand at 2589.094 kb on + strand at 2589.144 kb on + strand, within Dshi_2446 at 2589.252 kb on - strand, within Dshi_2446 at 2589.265 kb on + strand, within Dshi_2446 at 2589.273 kb on - strand, within Dshi_2446 at 2589.273 kb on - strand, within Dshi_2446 at 2589.273 kb on - strand, within Dshi_2446 at 2589.273 kb on - strand, within Dshi_2446 at 2589.424 kb on - strand, within Dshi_2446 at 2589.430 kb on + strand, within Dshi_2446 at 2589.440 kb on + strand, within Dshi_2446 at 2589.718 kb on + strand, within Dshi_2446 at 2589.741 kb on + strand, within Dshi_2446 at 2589.749 kb on - strand, within Dshi_2446 at 2589.779 kb on + strand, within Dshi_2446 at 2589.808 kb on - strand, within Dshi_2446 at 2589.812 kb on - strand, within Dshi_2446 at 2589.876 kb on - strand at 2589.901 kb on + strand at 2589.994 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 2,587,330 + +3.3 2,587,453 + Dshi_2444 0.16 -2.0 2,587,648 + Dshi_2444 0.35 -0.5 2,587,650 - Dshi_2444 0.35 +1.0 2,587,656 + Dshi_2444 0.36 +2.3 2,587,827 - Dshi_2444 0.53 +1.0 2,587,873 - Dshi_2444 0.57 +1.6 2,588,002 - Dshi_2444 0.70 -1.0 2,588,085 + Dshi_2444 0.79 -0.5 2,588,125 - Dshi_2444 0.82 -1.6 2,588,125 - Dshi_2444 0.82 -0.8 2,588,259 + -0.9 2,588,259 - +0.0 2,588,260 + -0.9 2,588,369 - -1.6 2,588,410 - Dshi_2445 0.15 +0.0 2,588,427 - Dshi_2445 0.18 -1.6 2,588,583 - Dshi_2445 0.40 -1.3 2,588,674 + Dshi_2445 0.52 -0.6 2,588,682 - Dshi_2445 0.54 +1.0 2,588,689 + Dshi_2445 0.54 -4.4 2,588,764 + Dshi_2445 0.65 -0.3 2,588,874 - Dshi_2445 0.80 -1.6 2,588,961 + -0.8 2,588,994 - +0.5 2,589,011 - -2.3 2,589,040 - -1.6 2,589,094 + +0.1 2,589,144 + Dshi_2446 0.14 -1.1 2,589,252 - Dshi_2446 0.26 -1.0 2,589,265 + Dshi_2446 0.27 -0.9 2,589,273 - Dshi_2446 0.28 -1.5 2,589,273 - Dshi_2446 0.28 -3.8 2,589,273 - Dshi_2446 0.28 -1.1 2,589,273 - Dshi_2446 0.28 -1.5 2,589,424 - Dshi_2446 0.45 -1.0 2,589,430 + Dshi_2446 0.46 -1.6 2,589,440 + Dshi_2446 0.47 -0.2 2,589,718 + Dshi_2446 0.77 -0.4 2,589,741 + Dshi_2446 0.80 -0.2 2,589,749 - Dshi_2446 0.80 -0.9 2,589,779 + Dshi_2446 0.84 -1.6 2,589,808 - Dshi_2446 0.87 +1.0 2,589,812 - Dshi_2446 0.87 -2.4 2,589,876 - -0.2 2,589,901 + +0.4 2,589,994 - -1.3
Or see this region's nucleotide sequence