Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2365 and Dshi_2366 are separated by 135 nucleotides Dshi_2366 and Dshi_2367 are separated by 112 nucleotides Dshi_2367 and Dshi_2368 overlap by 1 nucleotides Dshi_2368 and Dshi_2369 are separated by 131 nucleotides Dshi_2369 and Dshi_2370 are separated by 94 nucleotides
Dshi_2365: Dshi_2365 - urease, alpha subunit (RefSeq), at 2,498,640 to 2,500,349
_2365
Dshi_2366: Dshi_2366 - hypothetical protein (RefSeq), at 2,500,485 to 2,500,814
_2366
Dshi_2367: Dshi_2367 - alginate regulatory protein AlgP (RefSeq), at 2,500,927 to 2,501,649
_2367
Dshi_2368: Dshi_2368 - urease, beta subunit (RefSeq), at 2,501,649 to 2,501,954
_2368
Dshi_2369: Dshi_2369 - urease, gamma subunit (RefSeq), at 2,502,086 to 2,502,388
_2369
Dshi_2370: Dshi_2370 - Urease accessory protein UreD (RefSeq), at 2,502,483 to 2,503,328
_2370
Position (kb)
2500
2501
2502 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2500.082 kb on + strand, within Dshi_2365 at 2500.082 kb on + strand, within Dshi_2365 at 2500.096 kb on - strand, within Dshi_2365 at 2500.168 kb on + strand, within Dshi_2365 at 2500.176 kb on - strand, within Dshi_2365 at 2500.178 kb on + strand, within Dshi_2365 at 2500.306 kb on + strand at 2500.345 kb on + strand at 2500.439 kb on + strand at 2500.491 kb on - strand at 2500.540 kb on - strand, within Dshi_2366 at 2500.636 kb on + strand, within Dshi_2366 at 2500.636 kb on + strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.679 kb on - strand, within Dshi_2366 at 2500.747 kb on + strand, within Dshi_2366 at 2500.750 kb on - strand, within Dshi_2366 at 2500.789 kb on + strand at 2500.791 kb on - strand at 2500.791 kb on - strand at 2500.797 kb on - strand at 2500.802 kb on - strand at 2500.802 kb on - strand at 2500.817 kb on - strand at 2500.843 kb on + strand at 2500.908 kb on + strand at 2500.913 kb on + strand at 2500.970 kb on - strand at 2500.979 kb on + strand at 2500.979 kb on + strand at 2500.987 kb on - strand at 2501.033 kb on - strand, within Dshi_2367 at 2501.145 kb on - strand, within Dshi_2367 at 2501.296 kb on - strand, within Dshi_2367 at 2501.306 kb on + strand, within Dshi_2367 at 2501.307 kb on - strand, within Dshi_2367 at 2501.357 kb on + strand, within Dshi_2367 at 2501.363 kb on + strand, within Dshi_2367 at 2501.421 kb on - strand, within Dshi_2367 at 2501.446 kb on + strand, within Dshi_2367 at 2501.471 kb on + strand, within Dshi_2367 at 2501.479 kb on - strand, within Dshi_2367 at 2501.479 kb on - strand, within Dshi_2367 at 2501.545 kb on - strand, within Dshi_2367 at 2501.614 kb on + strand at 2501.623 kb on + strand at 2501.623 kb on - strand at 2501.631 kb on - strand at 2501.698 kb on + strand, within Dshi_2368 at 2501.713 kb on + strand, within Dshi_2368 at 2501.789 kb on - strand, within Dshi_2368 at 2501.803 kb on + strand, within Dshi_2368 at 2501.815 kb on + strand, within Dshi_2368 at 2501.852 kb on - strand, within Dshi_2368 at 2501.855 kb on - strand, within Dshi_2368 at 2501.860 kb on - strand, within Dshi_2368 at 2501.869 kb on + strand, within Dshi_2368 at 2501.881 kb on + strand, within Dshi_2368 at 2501.912 kb on + strand, within Dshi_2368 at 2501.990 kb on - strand at 2502.032 kb on + strand at 2502.032 kb on + strand at 2502.032 kb on + strand at 2502.032 kb on + strand at 2502.040 kb on - strand at 2502.040 kb on - strand at 2502.100 kb on + strand at 2502.252 kb on + strand, within Dshi_2369 at 2502.260 kb on - strand, within Dshi_2369 at 2502.275 kb on - strand, within Dshi_2369 at 2502.275 kb on - strand, within Dshi_2369 at 2502.294 kb on - strand, within Dshi_2369 at 2502.389 kb on - strand at 2502.404 kb on - strand at 2502.481 kb on + strand at 2502.489 kb on - strand at 2502.514 kb on - strand at 2502.591 kb on - strand, within Dshi_2370 at 2502.606 kb on + strand, within Dshi_2370 at 2502.629 kb on - strand, within Dshi_2370
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 2,500,082 + Dshi_2365 0.84 -2.2 2,500,082 + Dshi_2365 0.84 -1.9 2,500,096 - Dshi_2365 0.85 +1.3 2,500,168 + Dshi_2365 0.89 +0.2 2,500,176 - Dshi_2365 0.90 -2.7 2,500,178 + Dshi_2365 0.90 -2.4 2,500,306 + -1.1 2,500,345 + -0.0 2,500,439 + -0.0 2,500,491 - +3.0 2,500,540 - Dshi_2366 0.17 -0.5 2,500,636 + Dshi_2366 0.46 +1.0 2,500,636 + Dshi_2366 0.46 -0.7 2,500,644 - Dshi_2366 0.48 +1.4 2,500,644 - Dshi_2366 0.48 -1.9 2,500,644 - Dshi_2366 0.48 -3.6 2,500,679 - Dshi_2366 0.59 -1.6 2,500,747 + Dshi_2366 0.79 -0.0 2,500,750 - Dshi_2366 0.80 -1.1 2,500,789 + +1.0 2,500,791 - -2.1 2,500,791 - -3.4 2,500,797 - -2.8 2,500,802 - -1.3 2,500,802 - -0.0 2,500,817 - -1.4 2,500,843 + +3.0 2,500,908 + -2.8 2,500,913 + -2.8 2,500,970 - -1.4 2,500,979 + -1.0 2,500,979 + +0.1 2,500,987 - +0.2 2,501,033 - Dshi_2367 0.15 -0.0 2,501,145 - Dshi_2367 0.30 -0.0 2,501,296 - Dshi_2367 0.51 -0.0 2,501,306 + Dshi_2367 0.52 -1.0 2,501,307 - Dshi_2367 0.53 -0.6 2,501,357 + Dshi_2367 0.59 +1.0 2,501,363 + Dshi_2367 0.60 +1.0 2,501,421 - Dshi_2367 0.68 -1.8 2,501,446 + Dshi_2367 0.72 -1.4 2,501,471 + Dshi_2367 0.75 -0.8 2,501,479 - Dshi_2367 0.76 -0.7 2,501,479 - Dshi_2367 0.76 -0.0 2,501,545 - Dshi_2367 0.85 -0.3 2,501,614 + -2.0 2,501,623 + +2.0 2,501,623 - -0.4 2,501,631 - +0.2 2,501,698 + Dshi_2368 0.16 -2.0 2,501,713 + Dshi_2368 0.21 -1.0 2,501,789 - Dshi_2368 0.46 -3.5 2,501,803 + Dshi_2368 0.50 -1.6 2,501,815 + Dshi_2368 0.54 -1.2 2,501,852 - Dshi_2368 0.66 -2.2 2,501,855 - Dshi_2368 0.67 -0.6 2,501,860 - Dshi_2368 0.69 -0.5 2,501,869 + Dshi_2368 0.72 -0.0 2,501,881 + Dshi_2368 0.76 -1.2 2,501,912 + Dshi_2368 0.86 -0.8 2,501,990 - +1.6 2,502,032 + -0.5 2,502,032 + -2.0 2,502,032 + -2.4 2,502,032 + +0.5 2,502,040 - -0.0 2,502,040 - -0.0 2,502,100 + -2.8 2,502,252 + Dshi_2369 0.55 -2.5 2,502,260 - Dshi_2369 0.57 +1.5 2,502,275 - Dshi_2369 0.62 -3.1 2,502,275 - Dshi_2369 0.62 -1.9 2,502,294 - Dshi_2369 0.69 -1.6 2,502,389 - +1.0 2,502,404 - -0.0 2,502,481 + -2.8 2,502,489 - -3.5 2,502,514 - -1.0 2,502,591 - Dshi_2370 0.13 -0.0 2,502,606 + Dshi_2370 0.15 -0.0 2,502,629 - Dshi_2370 0.17 -2.6
Or see this region's nucleotide sequence