Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1987 and Dshi_1988 are separated by 99 nucleotides Dshi_1988 and Dshi_1989 are separated by 3 nucleotides Dshi_1989 and Dshi_R0040 are separated by 127 nucleotides Dshi_R0040 and Dshi_1990 are separated by 95 nucleotides
Dshi_1987: Dshi_1987 - hypothetical protein (RefSeq), at 2,102,173 to 2,102,721
_1987
Dshi_1988: Dshi_1988 - hypothetical protein (RefSeq), at 2,102,821 to 2,103,216
_1988
Dshi_1989: Dshi_1989 - secretion protein HlyD family protein (RefSeq), at 2,103,220 to 2,104,452
_1989
Dshi_R0040: Dshi_R0040 - tRNA-Arg (RefSeq), at 2,104,580 to 2,104,656
_R0040
Dshi_1990: Dshi_1990 - aminoglycoside phosphotransferase (RefSeq), at 2,104,752 to 2,106,995
_1990
Position (kb)
2103
2104
2105 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2102.258 kb on + strand, within Dshi_1987 at 2102.258 kb on + strand, within Dshi_1987 at 2102.266 kb on - strand, within Dshi_1987 at 2102.278 kb on + strand, within Dshi_1987 at 2102.286 kb on - strand, within Dshi_1987 at 2102.286 kb on - strand, within Dshi_1987 at 2102.466 kb on - strand, within Dshi_1987 at 2102.652 kb on + strand, within Dshi_1987 at 2102.868 kb on + strand, within Dshi_1988 at 2102.947 kb on - strand, within Dshi_1988 at 2102.968 kb on + strand, within Dshi_1988 at 2103.008 kb on - strand, within Dshi_1988 at 2103.200 kb on - strand at 2103.261 kb on - strand at 2103.296 kb on - strand at 2103.355 kb on - strand, within Dshi_1989 at 2103.371 kb on + strand, within Dshi_1989 at 2103.379 kb on - strand, within Dshi_1989 at 2103.421 kb on - strand at 2103.822 kb on - strand, within Dshi_1989 at 2103.887 kb on - strand, within Dshi_1989 at 2104.063 kb on + strand, within Dshi_1989 at 2104.164 kb on - strand, within Dshi_1989 at 2104.164 kb on - strand, within Dshi_1989 at 2104.230 kb on - strand, within Dshi_1989 at 2104.258 kb on - strand, within Dshi_1989 at 2104.322 kb on - strand, within Dshi_1989 at 2104.824 kb on + strand at 2104.849 kb on + strand at 2104.879 kb on - strand at 2105.007 kb on + strand, within Dshi_1990 at 2105.048 kb on + strand, within Dshi_1990 at 2105.172 kb on - strand at 2105.172 kb on - strand, within Dshi_1990 at 2105.294 kb on - strand, within Dshi_1990 at 2105.407 kb on + strand, within Dshi_1990
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 2,102,258 + Dshi_1987 0.15 -1.4 2,102,258 + Dshi_1987 0.15 -1.4 2,102,266 - Dshi_1987 0.17 +2.2 2,102,278 + Dshi_1987 0.19 -2.3 2,102,286 - Dshi_1987 0.21 +0.2 2,102,286 - Dshi_1987 0.21 -0.5 2,102,466 - Dshi_1987 0.53 -0.3 2,102,652 + Dshi_1987 0.87 +0.6 2,102,868 + Dshi_1988 0.12 +1.5 2,102,947 - Dshi_1988 0.32 -0.8 2,102,968 + Dshi_1988 0.37 +0.2 2,103,008 - Dshi_1988 0.47 +0.9 2,103,200 - -0.2 2,103,261 - -1.1 2,103,296 - +1.8 2,103,355 - Dshi_1989 0.11 +0.2 2,103,371 + Dshi_1989 0.12 -3.0 2,103,379 - Dshi_1989 0.13 +1.8 2,103,421 - +0.2 2,103,822 - Dshi_1989 0.49 -2.6 2,103,887 - Dshi_1989 0.54 -0.7 2,104,063 + Dshi_1989 0.68 -1.3 2,104,164 - Dshi_1989 0.77 -1.8 2,104,164 - Dshi_1989 0.77 -0.5 2,104,230 - Dshi_1989 0.82 -1.8 2,104,258 - Dshi_1989 0.84 +2.2 2,104,322 - Dshi_1989 0.89 +0.2 2,104,824 + -1.9 2,104,849 + -3.5 2,104,879 - -1.0 2,105,007 + Dshi_1990 0.11 -0.8 2,105,048 + Dshi_1990 0.13 -1.8 2,105,172 - +0.2 2,105,172 - Dshi_1990 0.19 -1.4 2,105,294 - Dshi_1990 0.24 -0.6 2,105,407 + Dshi_1990 0.29 -0.5
Or see this region's nucleotide sequence