Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1753 and Dshi_1754 are separated by 90 nucleotides Dshi_1754 and Dshi_1755 overlap by 8 nucleotides Dshi_1755 and Dshi_1756 overlap by 11 nucleotides Dshi_1756 and Dshi_1757 overlap by 10 nucleotides Dshi_1757 and Dshi_1758 are separated by 109 nucleotides
Dshi_1753: Dshi_1753 - Enoyl-CoA hydratase/isomerase (RefSeq), at 1,819,173 to 1,819,949
_1753
Dshi_1754: Dshi_1754 - hypothetical protein (RefSeq), at 1,820,040 to 1,820,456
_1754
Dshi_1755: Dshi_1755 - hypothetical protein (RefSeq), at 1,820,449 to 1,820,655
_1755
Dshi_1756: Dshi_1756 - hypothetical protein (RefSeq), at 1,820,645 to 1,821,166
_1756
Dshi_1757: Dshi_1757 - protein of unknown function DUF847 (RefSeq), at 1,821,157 to 1,821,768
_1757
Dshi_1758: Dshi_1758 - CcmE/CycJ protein (RefSeq), at 1,821,878 to 1,822,360
_1758
Position (kb)
1820
1821
1822 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1819.656 kb on + strand, within Dshi_1753 at 1819.766 kb on - strand, within Dshi_1753 at 1819.811 kb on - strand, within Dshi_1753 at 1819.936 kb on - strand at 1819.945 kb on + strand at 1819.953 kb on - strand at 1820.011 kb on - strand at 1820.188 kb on + strand, within Dshi_1754 at 1820.196 kb on - strand, within Dshi_1754 at 1820.202 kb on - strand, within Dshi_1754 at 1820.238 kb on - strand, within Dshi_1754 at 1820.366 kb on - strand, within Dshi_1754 at 1820.412 kb on + strand, within Dshi_1754 at 1820.420 kb on - strand at 1820.482 kb on + strand, within Dshi_1755 at 1820.515 kb on + strand, within Dshi_1755 at 1820.549 kb on - strand, within Dshi_1755 at 1820.892 kb on - strand, within Dshi_1756 at 1820.903 kb on + strand, within Dshi_1756 at 1820.970 kb on - strand, within Dshi_1756 at 1821.036 kb on - strand, within Dshi_1756 at 1821.102 kb on - strand, within Dshi_1756 at 1821.105 kb on + strand, within Dshi_1756 at 1821.215 kb on + strand at 1821.352 kb on - strand, within Dshi_1757 at 1821.417 kb on + strand, within Dshi_1757 at 1821.512 kb on - strand, within Dshi_1757 at 1821.519 kb on + strand, within Dshi_1757 at 1821.539 kb on - strand, within Dshi_1757 at 1821.759 kb on - strand at 1821.785 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 1,819,656 + Dshi_1753 0.62 -1.6 1,819,766 - Dshi_1753 0.76 -1.3 1,819,811 - Dshi_1753 0.82 +0.0 1,819,936 - +1.4 1,819,945 + -2.3 1,819,953 - -0.6 1,820,011 - -2.1 1,820,188 + Dshi_1754 0.35 +0.2 1,820,196 - Dshi_1754 0.37 +1.0 1,820,202 - Dshi_1754 0.39 -1.8 1,820,238 - Dshi_1754 0.47 -0.6 1,820,366 - Dshi_1754 0.78 -3.8 1,820,412 + Dshi_1754 0.89 -0.7 1,820,420 - +2.0 1,820,482 + Dshi_1755 0.16 -1.0 1,820,515 + Dshi_1755 0.32 +0.0 1,820,549 - Dshi_1755 0.48 -1.6 1,820,892 - Dshi_1756 0.47 +0.5 1,820,903 + Dshi_1756 0.49 -0.8 1,820,970 - Dshi_1756 0.62 -1.4 1,821,036 - Dshi_1756 0.75 +3.5 1,821,102 - Dshi_1756 0.88 -1.5 1,821,105 + Dshi_1756 0.88 -2.2 1,821,215 + -0.2 1,821,352 - Dshi_1757 0.32 +1.0 1,821,417 + Dshi_1757 0.42 -0.7 1,821,512 - Dshi_1757 0.58 +0.7 1,821,519 + Dshi_1757 0.59 -1.6 1,821,539 - Dshi_1757 0.62 +0.0 1,821,759 - -2.1 1,821,785 - +1.0
Or see this region's nucleotide sequence