Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1512 and Dshi_1513 are separated by 52 nucleotides Dshi_1513 and Dshi_1514 are separated by 79 nucleotides Dshi_1514 and Dshi_1515 are separated by 3 nucleotides Dshi_1515 and Dshi_1516 are separated by 60 nucleotides Dshi_1516 and Dshi_1517 are separated by 109 nucleotides
Dshi_1512: Dshi_1512 - cytochrome oxidase assembly (RefSeq), at 1,574,911 to 1,576,062
_1512
Dshi_1513: Dshi_1513 - tRNA/rRNA methyltransferase (SpoU) (RefSeq), at 1,576,115 to 1,576,882
_1513
Dshi_1514: Dshi_1514 - thiamine monophosphate synthase (RefSeq), at 1,576,962 to 1,577,585
_1514
Dshi_1515: Dshi_1515 - Phosphoribosyl-AMP cyclohydrolase (RefSeq), at 1,577,589 to 1,577,948
_1515
Dshi_1516: Dshi_1516 - nitrogen-fixing NifU domain protein (RefSeq), at 1,578,009 to 1,578,458
_1516
Dshi_1517: Dshi_1517 - inner-membrane translocator (RefSeq), at 1,578,568 to 1,579,872
_1517
Position (kb)
1576
1577
1578 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1576.096 kb on - strand at 1576.316 kb on - strand, within Dshi_1513 at 1576.406 kb on - strand, within Dshi_1513 at 1576.885 kb on + strand at 1576.885 kb on + strand at 1576.885 kb on + strand at 1576.893 kb on - strand at 1577.130 kb on - strand, within Dshi_1514 at 1577.270 kb on + strand, within Dshi_1514 at 1577.328 kb on + strand, within Dshi_1514 at 1577.328 kb on + strand, within Dshi_1514 at 1577.485 kb on + strand, within Dshi_1514 at 1577.533 kb on - strand at 1577.587 kb on + strand at 1578.024 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 1,576,096 - -0.1 1,576,316 - Dshi_1513 0.26 -1.5 1,576,406 - Dshi_1513 0.38 -0.7 1,576,885 + +2.6 1,576,885 + -0.7 1,576,885 + +0.2 1,576,893 - -2.1 1,577,130 - Dshi_1514 0.27 -2.7 1,577,270 + Dshi_1514 0.49 -0.5 1,577,328 + Dshi_1514 0.59 +0.3 1,577,328 + Dshi_1514 0.59 -0.9 1,577,485 + Dshi_1514 0.84 -0.9 1,577,533 - -0.4 1,577,587 + -1.0 1,578,024 - +0.7
Or see this region's nucleotide sequence