Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0948 and Dshi_0949 are separated by 51 nucleotides Dshi_0949 and Dshi_0950 are separated by 109 nucleotides Dshi_0950 and Dshi_0951 are separated by 209 nucleotides
Dshi_0948: Dshi_0948 - L-lactate dehydrogenase (cytochrome) (RefSeq), at 972,616 to 973,788
_0948
Dshi_0949: Dshi_0949 - hypothetical protein (RefSeq), at 973,840 to 974,457
_0949
Dshi_0950: Dshi_0950 - ribosomal 5S rRNA E-loop binding protein Ctc/L25/TL5 (RefSeq), at 974,567 to 975,202
_0950
Dshi_0951: Dshi_0951 - hypothetical protein (RefSeq), at 975,412 to 975,882
_0951
Position (kb) 974
975
976 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 973.608 kb on + strand, within Dshi_0948 at 973.616 kb on - strand, within Dshi_0948 at 973.616 kb on - strand, within Dshi_0948 at 973.741 kb on - strand at 973.889 kb on - strand at 973.889 kb on - strand at 973.903 kb on - strand, within Dshi_0949 at 974.001 kb on - strand, within Dshi_0949 at 974.013 kb on + strand, within Dshi_0949 at 974.070 kb on + strand, within Dshi_0949 at 974.168 kb on + strand, within Dshi_0949 at 974.235 kb on + strand, within Dshi_0949 at 974.243 kb on - strand, within Dshi_0949 at 974.413 kb on + strand at 974.439 kb on - strand at 975.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 973,608 + Dshi_0948 0.85 +3.0 973,616 - Dshi_0948 0.85 +0.2 973,616 - Dshi_0948 0.85 -2.3 973,741 - -0.0 973,889 - +1.2 973,889 - -1.6 973,903 - Dshi_0949 0.10 +1.2 974,001 - Dshi_0949 0.26 -0.2 974,013 + Dshi_0949 0.28 +1.2 974,070 + Dshi_0949 0.37 -1.8 974,168 + Dshi_0949 0.53 +2.6 974,235 + Dshi_0949 0.64 +2.0 974,243 - Dshi_0949 0.65 -0.4 974,413 + +2.0 974,439 - -0.6 975,219 - -1.5
Or see this region's nucleotide sequence