Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0719 and Dshi_0720 overlap by 8 nucleotides Dshi_0720 and Dshi_0721 overlap by 4 nucleotides Dshi_0721 and Dshi_0722 are separated by 14 nucleotides Dshi_0722 and Dshi_0723 are separated by 358 nucleotides
Dshi_0719: Dshi_0719 - hypothetical protein (RefSeq), at 727,888 to 728,286
_0719
Dshi_0720: Dshi_0720 - Endoribonuclease L-PSP (RefSeq), at 728,279 to 728,668
_0720
Dshi_0721: Dshi_0721 - hypothetical protein (RefSeq), at 728,665 to 729,036
_0721
Dshi_0722: Dshi_0722 - hypothetical protein (RefSeq), at 729,051 to 729,410
_0722
Dshi_0723: Dshi_0723 - Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) <small>(from data)</small>, at 729,769 to 731,814
_0723
Position (kb) 729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 728.062 kb on + strand, within Dshi_0719 at 728.226 kb on + strand, within Dshi_0719 at 728.236 kb on + strand, within Dshi_0719 at 728.409 kb on + strand, within Dshi_0720 at 728.440 kb on + strand, within Dshi_0720 at 728.562 kb on - strand, within Dshi_0720 at 728.562 kb on - strand, within Dshi_0720 at 728.580 kb on - strand, within Dshi_0720 at 728.643 kb on - strand at 728.890 kb on + strand, within Dshi_0721 at 729.192 kb on + strand, within Dshi_0722 at 729.192 kb on + strand, within Dshi_0722 at 729.304 kb on + strand, within Dshi_0722 at 729.347 kb on + strand, within Dshi_0722 at 729.376 kb on + strand at 729.416 kb on + strand at 729.544 kb on + strand at 729.562 kb on - strand at 729.684 kb on + strand at 729.694 kb on - strand at 729.817 kb on + strand at 729.863 kb on + strand at 729.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 728,062 + Dshi_0719 0.44 +0.3 728,226 + Dshi_0719 0.85 -1.1 728,236 + Dshi_0719 0.87 -0.2 728,409 + Dshi_0720 0.33 -0.7 728,440 + Dshi_0720 0.41 +0.3 728,562 - Dshi_0720 0.73 -2.8 728,562 - Dshi_0720 0.73 -1.4 728,580 - Dshi_0720 0.77 +1.0 728,643 - -1.2 728,890 + Dshi_0721 0.60 -0.7 729,192 + Dshi_0722 0.39 -2.5 729,192 + Dshi_0722 0.39 -2.2 729,304 + Dshi_0722 0.70 +0.3 729,347 + Dshi_0722 0.82 -0.7 729,376 + -3.1 729,416 + -1.7 729,544 + +1.3 729,562 - -0.4 729,684 + -2.0 729,694 - -0.4 729,817 + +1.3 729,863 + +0.0 729,871 - +0.3
Or see this region's nucleotide sequence