Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0688 and Dshi_0689 overlap by 4 nucleotides Dshi_0689 and Dshi_0690 overlap by 4 nucleotides Dshi_0690 and Dshi_0691 overlap by 4 nucleotides
Dshi_0688: Dshi_0688 - fumarate reductase/succinate dehydrogenase flavoprotein domain protein (RefSeq), at 693,474 to 694,838
_0688
Dshi_0689: Dshi_0689 - isocitrate lyase family protein (RefSeq), at 694,835 to 695,713
_0689
Dshi_0690: Dshi_0690 - isochorismatase hydrolase (RefSeq), at 695,710 to 696,312
_0690
Dshi_0691: Dshi_0691 - 5-oxoprolinase (ATP-hydrolyzing) (RefSeq), at 696,309 to 698,378
_0691
Position (kb) 694
695
696 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 693.860 kb on - strand, within Dshi_0688 at 693.969 kb on + strand, within Dshi_0688 at 694.060 kb on - strand, within Dshi_0688 at 694.090 kb on - strand, within Dshi_0688 at 694.112 kb on + strand, within Dshi_0688 at 694.121 kb on + strand, within Dshi_0688 at 694.190 kb on - strand, within Dshi_0688 at 694.241 kb on + strand, within Dshi_0688 at 694.284 kb on - strand, within Dshi_0688 at 694.293 kb on + strand, within Dshi_0688 at 694.388 kb on - strand, within Dshi_0688 at 694.536 kb on + strand, within Dshi_0688 at 694.576 kb on + strand, within Dshi_0688 at 694.650 kb on - strand, within Dshi_0688 at 694.697 kb on - strand, within Dshi_0688 at 694.715 kb on - strand at 694.947 kb on - strand at 694.958 kb on - strand, within Dshi_0689 at 694.983 kb on + strand, within Dshi_0689 at 694.991 kb on - strand, within Dshi_0689 at 694.991 kb on - strand, within Dshi_0689 at 695.026 kb on - strand, within Dshi_0689 at 695.034 kb on - strand, within Dshi_0689 at 695.120 kb on - strand, within Dshi_0689 at 695.158 kb on - strand, within Dshi_0689 at 695.168 kb on - strand, within Dshi_0689 at 695.220 kb on + strand, within Dshi_0689 at 695.220 kb on + strand, within Dshi_0689 at 695.220 kb on + strand, within Dshi_0689 at 695.228 kb on - strand at 695.232 kb on + strand, within Dshi_0689 at 695.261 kb on + strand, within Dshi_0689 at 695.323 kb on + strand, within Dshi_0689 at 695.367 kb on + strand, within Dshi_0689 at 695.375 kb on - strand, within Dshi_0689 at 695.375 kb on - strand, within Dshi_0689 at 695.377 kb on + strand, within Dshi_0689 at 695.435 kb on - strand, within Dshi_0689 at 695.458 kb on - strand, within Dshi_0689 at 695.492 kb on - strand, within Dshi_0689 at 695.545 kb on - strand, within Dshi_0689 at 695.566 kb on + strand, within Dshi_0689 at 695.568 kb on + strand, within Dshi_0689 at 695.593 kb on + strand, within Dshi_0689 at 695.767 kb on + strand at 695.775 kb on - strand, within Dshi_0690 at 695.775 kb on - strand, within Dshi_0690 at 695.793 kb on + strand, within Dshi_0690 at 695.852 kb on - strand, within Dshi_0690 at 695.861 kb on + strand, within Dshi_0690 at 695.944 kb on + strand, within Dshi_0690 at 695.944 kb on + strand, within Dshi_0690 at 695.952 kb on - strand, within Dshi_0690 at 695.957 kb on - strand, within Dshi_0690 at 695.994 kb on + strand, within Dshi_0690 at 696.001 kb on - strand, within Dshi_0690 at 696.042 kb on - strand, within Dshi_0690 at 696.232 kb on + strand, within Dshi_0690 at 696.236 kb on + strand, within Dshi_0690 at 696.340 kb on + strand at 696.369 kb on - strand at 696.421 kb on - strand at 696.447 kb on - strand at 696.447 kb on - strand at 696.544 kb on + strand, within Dshi_0691 at 696.622 kb on + strand, within Dshi_0691 at 696.628 kb on + strand, within Dshi_0691 at 696.628 kb on + strand, within Dshi_0691 at 696.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 693,860 - Dshi_0688 0.28 +1.3 693,969 + Dshi_0688 0.36 -1.7 694,060 - Dshi_0688 0.43 -2.3 694,090 - Dshi_0688 0.45 +2.0 694,112 + Dshi_0688 0.47 +1.3 694,121 + Dshi_0688 0.47 -0.7 694,190 - Dshi_0688 0.52 +2.3 694,241 + Dshi_0688 0.56 -1.9 694,284 - Dshi_0688 0.59 +0.3 694,293 + Dshi_0688 0.60 -1.7 694,388 - Dshi_0688 0.67 +1.9 694,536 + Dshi_0688 0.78 +0.3 694,576 + Dshi_0688 0.81 -2.0 694,650 - Dshi_0688 0.86 +0.3 694,697 - Dshi_0688 0.90 +0.3 694,715 - -0.2 694,947 - -0.7 694,958 - Dshi_0689 0.14 +0.3 694,983 + Dshi_0689 0.17 -0.3 694,991 - Dshi_0689 0.18 +1.9 694,991 - Dshi_0689 0.18 +0.9 695,026 - Dshi_0689 0.22 +1.3 695,034 - Dshi_0689 0.23 +1.9 695,120 - Dshi_0689 0.32 +0.3 695,158 - Dshi_0689 0.37 +1.3 695,168 - Dshi_0689 0.38 +0.3 695,220 + Dshi_0689 0.44 -0.7 695,220 + Dshi_0689 0.44 -2.0 695,220 + Dshi_0689 0.44 -1.2 695,228 - +0.3 695,232 + Dshi_0689 0.45 -2.3 695,261 + Dshi_0689 0.48 -0.1 695,323 + Dshi_0689 0.56 +0.3 695,367 + Dshi_0689 0.61 -2.1 695,375 - Dshi_0689 0.61 -1.6 695,375 - Dshi_0689 0.61 -2.2 695,377 + Dshi_0689 0.62 -1.9 695,435 - Dshi_0689 0.68 +1.6 695,458 - Dshi_0689 0.71 +1.9 695,492 - Dshi_0689 0.75 -2.0 695,545 - Dshi_0689 0.81 -0.9 695,566 + Dshi_0689 0.83 +2.3 695,568 + Dshi_0689 0.83 -1.3 695,593 + Dshi_0689 0.86 -1.3 695,767 + +0.0 695,775 - Dshi_0690 0.11 +1.3 695,775 - Dshi_0690 0.11 -3.9 695,793 + Dshi_0690 0.14 +0.2 695,852 - Dshi_0690 0.24 +2.6 695,861 + Dshi_0690 0.25 +1.3 695,944 + Dshi_0690 0.39 -2.7 695,944 + Dshi_0690 0.39 +1.9 695,952 - Dshi_0690 0.40 +1.1 695,957 - Dshi_0690 0.41 -0.8 695,994 + Dshi_0690 0.47 -0.1 696,001 - Dshi_0690 0.48 -1.4 696,042 - Dshi_0690 0.55 -0.1 696,232 + Dshi_0690 0.87 -1.0 696,236 + Dshi_0690 0.87 -1.5 696,340 + +2.6 696,369 - -1.5 696,421 - +1.3 696,447 - +2.6 696,447 - -1.9 696,544 + Dshi_0691 0.11 -1.3 696,622 + Dshi_0691 0.15 -0.3 696,628 + Dshi_0691 0.15 -2.5 696,628 + Dshi_0691 0.15 -2.3 696,708 + +1.3
Or see this region's nucleotide sequence