Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0589 and Dshi_0590 are separated by 33 nucleotides Dshi_0590 and Dshi_0591 are separated by 9 nucleotides Dshi_0591 and Dshi_0592 overlap by 4 nucleotides
Dshi_0589: Dshi_0589 - hypothetical protein (RefSeq), at 579,497 to 580,282
_0589
Dshi_0590: Dshi_0590 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 580,316 to 581,251
_0590
Dshi_0591: Dshi_0591 - rod shape-determining protein RodA (RefSeq), at 581,261 to 582,400
_0591
Dshi_0592: Dshi_0592 - Peptidoglycan glycosyltransferase (RefSeq), at 582,397 to 584,340
_0592
Position (kb) 581
582
583 Strain fitness (log2 ratio) -1
0
1
2 at 580.440 kb on + strand, within Dshi_0590 at 580.443 kb on - strand, within Dshi_0590 at 580.523 kb on - strand, within Dshi_0590 at 580.815 kb on + strand, within Dshi_0590 at 580.899 kb on + strand, within Dshi_0590 at 580.928 kb on + strand, within Dshi_0590 at 580.933 kb on + strand, within Dshi_0590 at 580.970 kb on - strand, within Dshi_0590 at 580.991 kb on + strand, within Dshi_0590 at 580.991 kb on + strand, within Dshi_0590 at 580.999 kb on - strand, within Dshi_0590 at 581.148 kb on + strand, within Dshi_0590 at 581.181 kb on - strand at 582.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 580,440 + Dshi_0590 0.13 +0.5 580,443 - Dshi_0590 0.14 +0.6 580,523 - Dshi_0590 0.22 +0.0 580,815 + Dshi_0590 0.53 -0.7 580,899 + Dshi_0590 0.62 +0.0 580,928 + Dshi_0590 0.65 -1.0 580,933 + Dshi_0590 0.66 -1.1 580,970 - Dshi_0590 0.70 -0.1 580,991 + Dshi_0590 0.72 +1.8 580,991 + Dshi_0590 0.72 -1.1 580,999 - Dshi_0590 0.73 +0.4 581,148 + Dshi_0590 0.89 +1.0 581,181 - -0.9 582,363 + -1.3
Or see this region's nucleotide sequence