Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0320 and Dshi_0321 are separated by 15 nucleotides Dshi_0321 and Dshi_0322 are separated by 43 nucleotides Dshi_0322 and Dshi_0323 overlap by 4 nucleotides Dshi_0323 and Dshi_0324 are separated by 52 nucleotides
Dshi_0320: Dshi_0320 - polar amino acid ABC transporter, inner membrane subunit (RefSeq), at 308,666 to 309,964
_0320
Dshi_0321: Dshi_0321 - ABC transporter related (RefSeq), at 309,980 to 310,756
_0321
Dshi_0322: Dshi_0322 - putative phosphohistidine phosphatase, SixA (RefSeq), at 310,800 to 311,300
_0322
Dshi_0323: Dshi_0323 - hypothetical protein (RefSeq), at 311,297 to 311,920
_0323
Dshi_0324: Dshi_0324 - acetylglutamate kinase (RefSeq), at 311,973 to 312,836
_0324
Position (kb) 310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 309.858 kb on + strand at 309.930 kb on + strand at 309.938 kb on - strand at 310.000 kb on - strand at 310.014 kb on + strand at 310.101 kb on + strand, within Dshi_0321 at 310.156 kb on + strand, within Dshi_0321 at 310.254 kb on + strand, within Dshi_0321 at 310.254 kb on + strand, within Dshi_0321 at 310.257 kb on - strand, within Dshi_0321 at 310.548 kb on - strand, within Dshi_0321 at 311.516 kb on + strand, within Dshi_0323 at 311.523 kb on + strand, within Dshi_0323 at 311.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 309,858 + +0.1 309,930 + -0.9 309,938 - -2.3 310,000 - -1.6 310,014 + -1.3 310,101 + Dshi_0321 0.16 -1.7 310,156 + Dshi_0321 0.23 -0.1 310,254 + Dshi_0321 0.35 -1.1 310,254 + Dshi_0321 0.35 -2.3 310,257 - Dshi_0321 0.36 -2.9 310,548 - Dshi_0321 0.73 -1.0 311,516 + Dshi_0323 0.35 +0.3 311,523 + Dshi_0323 0.36 -0.4 311,977 - -0.3
Or see this region's nucleotide sequence