Experiment: m.b. A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0253 and Dshi_0254 are separated by 30 nucleotides Dshi_0254 and Dshi_0255 are separated by 156 nucleotides Dshi_0255 and Dshi_0256 are separated by 78 nucleotides Dshi_0256 and Dshi_0257 are separated by 59 nucleotides
Dshi_0253: Dshi_0253 - AMP-dependent synthetase and ligase (RefSeq), at 251,200 to 252,759
_0253
Dshi_0254: Dshi_0254 - ribonuclease T2 (RefSeq), at 252,790 to 253,431
_0254
Dshi_0255: Dshi_0255 - protein of unknown function DUF1013 (RefSeq), at 253,588 to 254,340
_0255
Dshi_0256: Dshi_0256 - hypothetical protein (RefSeq), at 254,419 to 254,667
_0256
Dshi_0257: Dshi_0257 - peptidase S8 and S53 subtilisin kexin sedolisin (RefSeq), at 254,727 to 256,076
_0257
Position (kb) 253
254
255 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 252.617 kb on + strand at 252.668 kb on + strand at 252.737 kb on - strand at 252.748 kb on + strand at 252.793 kb on + strand at 252.796 kb on - strand at 252.869 kb on - strand, within Dshi_0254 at 252.878 kb on + strand, within Dshi_0254 at 252.892 kb on + strand, within Dshi_0254 at 252.939 kb on - strand, within Dshi_0254 at 253.305 kb on + strand, within Dshi_0254 at 253.370 kb on - strand at 253.388 kb on - strand at 253.416 kb on - strand at 253.419 kb on - strand at 254.171 kb on + strand, within Dshi_0255 at 254.320 kb on - strand at 254.413 kb on - strand at 254.687 kb on - strand at 254.799 kb on - strand at 254.873 kb on - strand, within Dshi_0257 at 254.955 kb on + strand, within Dshi_0257 at 255.010 kb on - strand, within Dshi_0257 at 255.017 kb on - strand, within Dshi_0257 at 255.088 kb on + strand, within Dshi_0257 at 255.231 kb on - strand, within Dshi_0257 at 255.244 kb on + strand, within Dshi_0257 at 255.306 kb on + strand, within Dshi_0257
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.01 mg/ml remove 252,617 + -0.2 252,668 + +2.4 252,737 - -2.2 252,748 + -0.3 252,793 + -0.8 252,796 - +1.0 252,869 - Dshi_0254 0.12 +1.4 252,878 + Dshi_0254 0.14 -0.3 252,892 + Dshi_0254 0.16 -0.5 252,939 - Dshi_0254 0.23 +0.4 253,305 + Dshi_0254 0.80 -1.7 253,370 - -1.1 253,388 - +1.4 253,416 - +0.1 253,419 - +2.7 254,171 + Dshi_0255 0.77 -0.3 254,320 - +0.2 254,413 - -1.4 254,687 - +2.0 254,799 - -0.3 254,873 - Dshi_0257 0.11 +2.7 254,955 + Dshi_0257 0.17 -0.2 255,010 - Dshi_0257 0.21 +0.9 255,017 - Dshi_0257 0.21 -0.6 255,088 + Dshi_0257 0.27 +0.7 255,231 - Dshi_0257 0.37 -0.8 255,244 + Dshi_0257 0.38 +3.4 255,306 + Dshi_0257 0.43 +1.4
Or see this region's nucleotide sequence