Experiment: D-Glucosamine Hydrochloride as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1245 and HP15_1246 are separated by 165 nucleotides HP15_1246 and HP15_rRNA7 are separated by 208 nucleotides HP15_rRNA7 and HP15_tRNA20 are separated by 92 nucleotides HP15_tRNA20 and HP15_tRNA21 are separated by 122 nucleotides HP15_tRNA21 and HP15_rRNA8 are separated by 340 nucleotides
HP15_1245: HP15_1245 - phosphoribosylglycinamide formyltransferase 2, at 1,297,110 to 1,298,300
_1245
HP15_1246: HP15_1246 - hypothetical protein, at 1,298,466 to 1,298,612
_1246
HP15_rRNA7: HP15_rRNA7 - 16S ribosomal RNA, at 1,298,821 to 1,300,352
_rRNA7
HP15_tRNA20: HP15_tRNA20 - tRNA-Ile, at 1,300,445 to 1,300,521
_tRNA20
HP15_tRNA21: HP15_tRNA21 - tRNA-Ala, at 1,300,644 to 1,300,719
_tRNA21
HP15_rRNA8: HP15_rRNA8 - 23S ribosomal RNA, at 1,301,060 to 1,303,951
_rRNA8
Position (kb) 1298
1299
1300
1301 Strain fitness (log2 ratio) -1
0
1
2 at 1297.920 kb on - strand, within HP15_1245 at 1297.975 kb on + strand, within HP15_1245 at 1297.983 kb on - strand, within HP15_1245 at 1297.983 kb on - strand, within HP15_1245 at 1297.993 kb on - strand, within HP15_1245 at 1298.007 kb on - strand, within HP15_1245 at 1298.051 kb on - strand, within HP15_1245 at 1298.148 kb on - strand, within HP15_1245 at 1298.287 kb on + strand at 1298.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride as (N) remove 1,297,920 - HP15_1245 0.68 +0.0 1,297,975 + HP15_1245 0.73 +2.0 1,297,983 - HP15_1245 0.73 +1.9 1,297,983 - HP15_1245 0.73 +0.1 1,297,993 - HP15_1245 0.74 -0.3 1,298,007 - HP15_1245 0.75 -0.2 1,298,051 - HP15_1245 0.79 +2.0 1,298,148 - HP15_1245 0.87 -0.1 1,298,287 + -0.1 1,298,305 + -0.3
Or see this region's nucleotide sequence