Experiment: MoYLS4 with Chloramphenicol 0.003125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3160 and DvMF_3161 are separated by 4 nucleotides DvMF_3161 and DvMF_3162 are separated by 138 nucleotides DvMF_3162 and DvMF_3163 are separated by 350 nucleotides
DvMF_3160: DvMF_3160 - Nucleoside-diphosphate kinase (RefSeq), at 3,986,241 to 3,986,660
_3160
DvMF_3161: DvMF_3161 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 3,986,665 to 3,987,474
_3161
DvMF_3162: DvMF_3162 - Smr protein/MutS2 (RefSeq), at 3,987,613 to 3,988,965
_3162
DvMF_3163: DvMF_3163 - signal peptide peptidase SppA, 36K type (RefSeq), at 3,989,316 to 3,990,323
_3163
Position (kb)
3987
3988
3989 Strain fitness (log2 ratio)
-1
0
1 at 3986.934 kb on + strand, within DvMF_3161 at 3987.042 kb on + strand, within DvMF_3161 at 3987.050 kb on - strand, within DvMF_3161 at 3987.191 kb on + strand, within DvMF_3161 at 3987.276 kb on + strand, within DvMF_3161 at 3987.739 kb on - strand at 3987.786 kb on - strand, within DvMF_3162 at 3987.868 kb on - strand, within DvMF_3162 at 3987.873 kb on - strand, within DvMF_3162 at 3987.930 kb on + strand, within DvMF_3162 at 3987.952 kb on + strand, within DvMF_3162 at 3988.155 kb on + strand, within DvMF_3162 at 3988.485 kb on + strand, within DvMF_3162 at 3988.954 kb on + strand at 3989.159 kb on - strand at 3989.183 kb on - strand at 3989.482 kb on - strand, within DvMF_3163 at 3989.745 kb on + strand, within DvMF_3163 at 3989.796 kb on - strand, within DvMF_3163 at 3989.854 kb on + strand, within DvMF_3163
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Chloramphenicol 0.003125 mg/ml remove 3,986,934 + DvMF_3161 0.33 -0.6 3,987,042 + DvMF_3161 0.47 -0.3 3,987,050 - DvMF_3161 0.48 -0.1 3,987,191 + DvMF_3161 0.65 -0.9 3,987,276 + DvMF_3161 0.75 -0.2 3,987,739 - -0.9 3,987,786 - DvMF_3162 0.13 -1.1 3,987,868 - DvMF_3162 0.19 -0.8 3,987,873 - DvMF_3162 0.19 -0.7 3,987,930 + DvMF_3162 0.23 -0.7 3,987,952 + DvMF_3162 0.25 -0.6 3,988,155 + DvMF_3162 0.40 -0.9 3,988,485 + DvMF_3162 0.64 -0.5 3,988,954 + -0.6 3,989,159 - -0.2 3,989,183 - +0.7 3,989,482 - DvMF_3163 0.16 -0.1 3,989,745 + DvMF_3163 0.43 -0.4 3,989,796 - DvMF_3163 0.48 -0.5 3,989,854 + DvMF_3163 0.53 -1.1
Or see this region's nucleotide sequence