Experiment: copper (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2050 and Shew_2051 are separated by 140 nucleotides Shew_2051 and Shew_2052 are separated by 8 nucleotides Shew_2052 and Shew_2053 are separated by 219 nucleotides
Shew_2050: Shew_2050 - pyruvate kinase (RefSeq), at 2,366,431 to 2,367,870
_2050
Shew_2051: Shew_2051 - acyl-CoA dehydrogenase (RefSeq), at 2,368,011 to 2,370,287
_2051
Shew_2052: Shew_2052 - TetR family transcriptional regulator (RefSeq), at 2,370,296 to 2,370,934
_2052
Shew_2053: Shew_2053 - peptidyl-dipeptidase A (RefSeq), at 2,371,154 to 2,373,058
_2053
Position (kb)
2368
2369
2370
2371 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2367.558 kb on + strand, within Shew_2050 at 2368.106 kb on + strand at 2368.201 kb on + strand at 2368.209 kb on - strand at 2368.209 kb on - strand at 2368.487 kb on - strand, within Shew_2051 at 2369.616 kb on - strand, within Shew_2051 at 2370.059 kb on + strand, within Shew_2051 at 2370.067 kb on - strand at 2370.273 kb on + strand at 2370.418 kb on - strand, within Shew_2052 at 2370.593 kb on + strand, within Shew_2052
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 0.5 mM remove 2,367,558 + Shew_2050 0.78 +0.8 2,368,106 + -0.4 2,368,201 + +0.1 2,368,209 - -0.2 2,368,209 - +0.1 2,368,487 - Shew_2051 0.21 -0.4 2,369,616 - Shew_2051 0.70 -0.4 2,370,059 + Shew_2051 0.90 -1.2 2,370,067 - +0.2 2,370,273 + -1.8 2,370,418 - Shew_2052 0.19 +0.4 2,370,593 + Shew_2052 0.46 +4.2
Or see this region's nucleotide sequence