Experiment: sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0747 and Shew_0748 are separated by 121 nucleotides Shew_0748 and Shew_0749 are separated by 71 nucleotides Shew_0749 and Shew_0750 are separated by 62 nucleotides Shew_0750 and Shew_0751 are separated by 69 nucleotides
Shew_0747: Shew_0747 - GGDEF domain-containing protein (RefSeq), at 847,984 to 848,940
_0747
Shew_0748: Shew_0748 - acetyltransferase (RefSeq), at 849,062 to 849,622
_0748
Shew_0749: Shew_0749 - hypothetical protein (RefSeq), at 849,694 to 850,038
_0749
Shew_0750: Shew_0750 - hypothetical protein (RefSeq), at 850,101 to 850,538
_0750
Shew_0751: Shew_0751 - S-adenosylmethionine synthetase (RefSeq), at 850,608 to 851,759
_0751
Position (kb) 849
850
851 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 848.731 kb on + strand, within Shew_0747 at 848.946 kb on + strand at 849.039 kb on + strand at 849.141 kb on + strand, within Shew_0748 at 849.330 kb on - strand, within Shew_0748 at 849.404 kb on - strand, within Shew_0748 at 849.417 kb on + strand, within Shew_0748 at 849.417 kb on + strand, within Shew_0748 at 849.543 kb on - strand, within Shew_0748 at 849.617 kb on + strand at 849.775 kb on - strand, within Shew_0749 at 849.838 kb on - strand, within Shew_0749 at 850.103 kb on + strand at 850.111 kb on + strand at 850.119 kb on - strand at 850.189 kb on - strand, within Shew_0750 at 850.431 kb on + strand, within Shew_0750
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 100 mM remove 848,731 + Shew_0747 0.78 -0.3 848,946 + +0.1 849,039 + +0.1 849,141 + Shew_0748 0.14 -0.0 849,330 - Shew_0748 0.48 -1.1 849,404 - Shew_0748 0.61 -0.2 849,417 + Shew_0748 0.63 -0.0 849,417 + Shew_0748 0.63 -2.8 849,543 - Shew_0748 0.86 -1.2 849,617 + +0.5 849,775 - Shew_0749 0.23 -0.3 849,838 - Shew_0749 0.42 -1.1 850,103 + +0.6 850,111 + -0.2 850,119 - +0.7 850,189 - Shew_0750 0.20 -0.2 850,431 + Shew_0750 0.75 -0.1
Or see this region's nucleotide sequence