Experiment: casaminos as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_811 and HP15_812 are separated by 44 nucleotides HP15_812 and HP15_813 are separated by 74 nucleotides HP15_813 and HP15_814 are separated by 29 nucleotides
HP15_811: HP15_811 - acetyl-CoA synthetase, at 829,378 to 831,327
_811
HP15_812: HP15_812 - two component transcriptional regulator, LuxR family, at 831,372 to 832,037
_812
HP15_813: HP15_813 - D-isomer specific 2-hydroxyacid dehydrogenase family protein, at 832,112 to 833,281
_813
HP15_814: HP15_814 - aspartate aminotransferase, at 833,311 to 834,561
_814
Position (kb)
832
833
834 Strain fitness (log2 ratio)
-1
0
1
2 at 832.138 kb on - strand at 832.450 kb on + strand, within HP15_813 at 832.457 kb on + strand, within HP15_813 at 832.555 kb on + strand, within HP15_813 at 832.829 kb on - strand, within HP15_813 at 832.829 kb on - strand, within HP15_813 at 832.841 kb on + strand, within HP15_813 at 832.849 kb on - strand, within HP15_813 at 832.953 kb on + strand, within HP15_813 at 832.975 kb on + strand, within HP15_813 at 832.975 kb on + strand, within HP15_813 at 833.084 kb on - strand, within HP15_813 at 833.150 kb on + strand, within HP15_813 at 833.150 kb on + strand, within HP15_813 at 833.164 kb on + strand, within HP15_813 at 833.267 kb on + strand at 833.283 kb on - strand at 833.293 kb on - strand at 833.316 kb on - strand at 833.339 kb on - strand at 833.391 kb on - strand at 833.645 kb on + strand, within HP15_814 at 833.669 kb on - strand, within HP15_814 at 833.769 kb on - strand, within HP15_814 at 833.784 kb on - strand, within HP15_814 at 833.820 kb on - strand, within HP15_814 at 833.882 kb on - strand, within HP15_814 at 833.934 kb on - strand, within HP15_814 at 833.993 kb on + strand, within HP15_814 at 834.009 kb on - strand, within HP15_814 at 834.043 kb on + strand, within HP15_814 at 834.229 kb on + strand, within HP15_814
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos as (N) remove 832,138 - +0.9 832,450 + HP15_813 0.29 -0.2 832,457 + HP15_813 0.29 +2.4 832,555 + HP15_813 0.38 -1.0 832,829 - HP15_813 0.61 +0.5 832,829 - HP15_813 0.61 +0.9 832,841 + HP15_813 0.62 +1.8 832,849 - HP15_813 0.63 -0.3 832,953 + HP15_813 0.72 +0.5 832,975 + HP15_813 0.74 +1.6 832,975 + HP15_813 0.74 +2.3 833,084 - HP15_813 0.83 +0.1 833,150 + HP15_813 0.89 -0.6 833,150 + HP15_813 0.89 +0.4 833,164 + HP15_813 0.90 +2.0 833,267 + +1.7 833,283 - -0.2 833,293 - -0.4 833,316 - +0.1 833,339 - -1.0 833,391 - -0.2 833,645 + HP15_814 0.27 +2.1 833,669 - HP15_814 0.29 -0.4 833,769 - HP15_814 0.37 -0.2 833,784 - HP15_814 0.38 -0.9 833,820 - HP15_814 0.41 -1.0 833,882 - HP15_814 0.46 -0.4 833,934 - HP15_814 0.50 -0.2 833,993 + HP15_814 0.55 +2.4 834,009 - HP15_814 0.56 -1.6 834,043 + HP15_814 0.59 +1.6 834,229 + HP15_814 0.73 -0.1
Or see this region's nucleotide sequence