Experiment: casaminos as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_168 and HP15_169 are separated by 87 nucleotides HP15_169 and HP15_171 are separated by 230 nucleotides HP15_171 and HP15_170 are separated by 45 nucleotides HP15_170 and HP15_172 are separated by 41 nucleotides HP15_172 and HP15_173 overlap by 17 nucleotides
HP15_168: HP15_168 - conserved hypothetical protein, at 163,409 to 163,513
_168
HP15_169: HP15_169 - hypothetical protein, at 163,601 to 163,714
_169
HP15_171: HP15_171 - cytochrome c, class I-like protein, at 163,945 to 164,073
_171
HP15_170: HP15_170 - hypothetical protein, at 164,119 to 164,322
_170
HP15_172: HP15_172 - protein-S-isoprenylcysteine methyltransferase, at 164,364 to 165,032
_172
HP15_173: HP15_173 - secreted protein, at 165,016 to 165,189
_173
Position (kb)
163
164
165 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 162.967 kb on - strand at 162.987 kb on + strand at 163.301 kb on - strand at 163.327 kb on + strand at 163.327 kb on + strand at 163.327 kb on + strand at 163.334 kb on + strand at 163.334 kb on + strand at 163.335 kb on - strand at 163.335 kb on - strand at 163.335 kb on - strand at 163.342 kb on - strand at 163.352 kb on - strand at 163.459 kb on + strand, within HP15_168 at 163.460 kb on + strand, within HP15_168 at 163.471 kb on + strand, within HP15_168 at 163.471 kb on + strand, within HP15_168 at 163.471 kb on + strand, within HP15_168 at 163.478 kb on + strand, within HP15_168 at 163.478 kb on - strand, within HP15_168 at 163.508 kb on + strand at 163.516 kb on - strand at 163.562 kb on + strand at 163.562 kb on + strand at 163.562 kb on + strand at 163.570 kb on - strand at 163.570 kb on - strand at 163.606 kb on + strand at 163.829 kb on - strand at 163.897 kb on - strand at 163.967 kb on - strand, within HP15_171 at 164.008 kb on + strand, within HP15_171 at 164.030 kb on + strand, within HP15_171 at 164.038 kb on - strand, within HP15_171 at 164.038 kb on - strand, within HP15_171 at 164.038 kb on - strand, within HP15_171 at 164.128 kb on + strand at 164.141 kb on - strand, within HP15_170 at 164.242 kb on + strand, within HP15_170 at 164.255 kb on - strand, within HP15_170 at 164.258 kb on + strand, within HP15_170 at 164.264 kb on + strand, within HP15_170 at 164.264 kb on + strand, within HP15_170 at 164.266 kb on + strand, within HP15_170 at 164.269 kb on + strand, within HP15_170 at 164.272 kb on - strand, within HP15_170 at 164.272 kb on - strand, within HP15_170 at 164.274 kb on + strand, within HP15_170 at 164.276 kb on + strand, within HP15_170 at 164.282 kb on - strand, within HP15_170 at 164.291 kb on + strand, within HP15_170 at 164.295 kb on - strand, within HP15_170 at 164.432 kb on - strand, within HP15_172 at 164.514 kb on - strand, within HP15_172 at 164.543 kb on + strand, within HP15_172 at 164.543 kb on + strand, within HP15_172 at 164.543 kb on + strand, within HP15_172 at 164.543 kb on + strand, within HP15_172 at 164.543 kb on + strand, within HP15_172 at 164.551 kb on - strand, within HP15_172 at 164.551 kb on - strand, within HP15_172 at 164.561 kb on - strand, within HP15_172 at 164.656 kb on + strand, within HP15_172 at 164.664 kb on - strand, within HP15_172 at 164.675 kb on - strand, within HP15_172 at 164.703 kb on + strand, within HP15_172 at 164.759 kb on - strand, within HP15_172 at 164.787 kb on + strand, within HP15_172 at 164.787 kb on + strand, within HP15_172 at 164.820 kb on + strand, within HP15_172 at 164.939 kb on - strand, within HP15_172 at 165.036 kb on + strand, within HP15_173 at 165.044 kb on - strand, within HP15_173 at 165.044 kb on - strand, within HP15_173 at 165.057 kb on + strand, within HP15_173
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos as (N) remove 162,967 - -0.9 162,987 + -0.9 163,301 - +2.1 163,327 + -0.3 163,327 + -0.1 163,327 + +1.5 163,334 + +0.0 163,334 + -2.4 163,335 - -0.2 163,335 - -2.2 163,335 - +0.8 163,342 - +2.3 163,352 - -0.1 163,459 + HP15_168 0.48 +1.6 163,460 + HP15_168 0.49 -0.7 163,471 + HP15_168 0.59 +0.4 163,471 + HP15_168 0.59 -0.2 163,471 + HP15_168 0.59 -1.0 163,478 + HP15_168 0.66 -0.4 163,478 - HP15_168 0.66 -0.7 163,508 + +3.0 163,516 - +0.0 163,562 + -0.5 163,562 + +2.4 163,562 + +0.1 163,570 - +1.0 163,570 - -0.9 163,606 + +2.0 163,829 - +0.7 163,897 - -0.8 163,967 - HP15_171 0.17 -1.5 164,008 + HP15_171 0.49 -0.6 164,030 + HP15_171 0.66 +0.3 164,038 - HP15_171 0.72 +0.2 164,038 - HP15_171 0.72 +2.0 164,038 - HP15_171 0.72 -0.9 164,128 + +2.7 164,141 - HP15_170 0.11 -0.3 164,242 + HP15_170 0.60 -0.5 164,255 - HP15_170 0.67 -0.2 164,258 + HP15_170 0.68 -0.7 164,264 + HP15_170 0.71 -0.2 164,264 + HP15_170 0.71 -0.1 164,266 + HP15_170 0.72 +2.0 164,269 + HP15_170 0.74 +2.7 164,272 - HP15_170 0.75 +1.5 164,272 - HP15_170 0.75 +1.8 164,274 + HP15_170 0.76 +1.0 164,276 + HP15_170 0.77 -0.5 164,282 - HP15_170 0.80 -0.3 164,291 + HP15_170 0.84 -1.3 164,295 - HP15_170 0.86 -1.3 164,432 - HP15_172 0.10 +1.4 164,514 - HP15_172 0.22 +0.0 164,543 + HP15_172 0.27 -0.4 164,543 + HP15_172 0.27 -0.3 164,543 + HP15_172 0.27 -0.2 164,543 + HP15_172 0.27 -0.6 164,543 + HP15_172 0.27 -0.7 164,551 - HP15_172 0.28 -0.1 164,551 - HP15_172 0.28 -0.1 164,561 - HP15_172 0.29 -0.7 164,656 + HP15_172 0.44 +1.4 164,664 - HP15_172 0.45 +0.0 164,675 - HP15_172 0.46 +1.9 164,703 + HP15_172 0.51 +0.4 164,759 - HP15_172 0.59 +0.1 164,787 + HP15_172 0.63 -1.4 164,787 + HP15_172 0.63 +1.8 164,820 + HP15_172 0.68 -0.5 164,939 - HP15_172 0.86 -0.5 165,036 + HP15_173 0.11 +1.3 165,044 - HP15_173 0.16 -0.1 165,044 - HP15_173 0.16 +0.7 165,057 + HP15_173 0.24 +1.5
Or see this region's nucleotide sequence