Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1961 and Shew_1962 are separated by 107 nucleotides Shew_1962 and Shew_1963 are separated by 34 nucleotides Shew_1963 and Shew_1964 are separated by 230 nucleotides Shew_1964 and pssA are separated by 11 nucleotides
Shew_1961: Shew_1961 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,265,581 to 2,267,332
_1961
Shew_1962: Shew_1962 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq), at 2,267,440 to 2,269,071
_1962
Shew_1963: Shew_1963 - hypothetical protein (RefSeq), at 2,269,106 to 2,269,282
_1963
Shew_1964: Shew_1964 - hypothetical protein (RefSeq), at 2,269,513 to 2,269,845
_1964
Shew_1965: pssA - phosphatidylserine synthase (RefSeq), at 2,269,857 to 2,271,170
pssA
Position (kb)
2267
2268
2269
2270 Strain fitness (log2 ratio)
-1
0
1
2 at 2266.496 kb on - strand, within Shew_1961 at 2267.254 kb on + strand at 2267.262 kb on - strand at 2267.897 kb on - strand, within Shew_1962 at 2267.978 kb on + strand, within Shew_1962 at 2268.746 kb on - strand, within Shew_1962 at 2269.283 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,266,496 - Shew_1961 0.52 -0.4 2,267,254 + -0.2 2,267,262 - +0.7 2,267,897 - Shew_1962 0.28 +1.8 2,267,978 + Shew_1962 0.33 +0.5 2,268,746 - Shew_1962 0.80 +0.0 2,269,283 + +0.6
Or see this region's nucleotide sequence