Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4133 and Dshi_4134 overlap by 4 nucleotides Dshi_4134 and Dshi_4135 overlap by 11 nucleotides Dshi_4135 and Dshi_4136 overlap by 8 nucleotides
Dshi_4133: Dshi_4133 - Oligosaccharide biosynthesis protein Alg14-like protein (RefSeq), at 65,809 to 66,255
_4133
Dshi_4134: Dshi_4134 - Glycosyltransferase 28 domain (RefSeq), at 66,252 to 66,728
_4134
Dshi_4135: Dshi_4135 - hypothetical protein (RefSeq), at 66,718 to 68,040
_4135
Dshi_4136: Dshi_4136 - polysaccharide pyruvyl transferase (RefSeq), at 68,033 to 69,217
_4136
Position (kb) 66
67
68
69 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 65.724 kb on + strand at 65.799 kb on + strand at 65.847 kb on - strand at 65.849 kb on + strand at 65.908 kb on + strand, within Dshi_4133 at 65.931 kb on + strand, within Dshi_4133 at 65.931 kb on + strand, within Dshi_4133 at 65.939 kb on - strand, within Dshi_4133 at 65.989 kb on + strand, within Dshi_4133 at 65.989 kb on + strand, within Dshi_4133 at 65.989 kb on + strand, within Dshi_4133 at 65.997 kb on - strand, within Dshi_4133 at 66.062 kb on - strand, within Dshi_4133 at 66.096 kb on + strand, within Dshi_4133 at 66.368 kb on + strand, within Dshi_4134 at 66.440 kb on + strand, within Dshi_4134 at 66.493 kb on - strand, within Dshi_4134 at 66.914 kb on + strand, within Dshi_4135 at 66.958 kb on + strand, within Dshi_4135 at 67.134 kb on + strand, within Dshi_4135 at 67.188 kb on - strand, within Dshi_4135 at 67.507 kb on + strand, within Dshi_4135 at 67.575 kb on - strand, within Dshi_4135 at 67.630 kb on + strand, within Dshi_4135 at 68.060 kb on + strand at 68.204 kb on + strand, within Dshi_4136 at 68.225 kb on - strand, within Dshi_4136 at 68.587 kb on + strand, within Dshi_4136 at 68.595 kb on - strand, within Dshi_4136 at 68.637 kb on + strand, within Dshi_4136 at 68.787 kb on + strand, within Dshi_4136 at 68.795 kb on - strand, within Dshi_4136 at 68.795 kb on - strand, within Dshi_4136 at 68.853 kb on + strand, within Dshi_4136 at 68.951 kb on - strand, within Dshi_4136 at 68.981 kb on + strand, within Dshi_4136 at 69.034 kb on - strand, within Dshi_4136
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 65,724 + +1.6 65,799 + +1.0 65,847 - -2.5 65,849 + -0.3 65,908 + Dshi_4133 0.22 +0.6 65,931 + Dshi_4133 0.27 -2.6 65,931 + Dshi_4133 0.27 +2.5 65,939 - Dshi_4133 0.29 -1.0 65,989 + Dshi_4133 0.40 -0.4 65,989 + Dshi_4133 0.40 -1.7 65,989 + Dshi_4133 0.40 -3.9 65,997 - Dshi_4133 0.42 +4.7 66,062 - Dshi_4133 0.57 -1.2 66,096 + Dshi_4133 0.64 -1.0 66,368 + Dshi_4134 0.24 -2.9 66,440 + Dshi_4134 0.39 -1.4 66,493 - Dshi_4134 0.51 -0.7 66,914 + Dshi_4135 0.15 -0.0 66,958 + Dshi_4135 0.18 -0.4 67,134 + Dshi_4135 0.31 -0.3 67,188 - Dshi_4135 0.36 -1.4 67,507 + Dshi_4135 0.60 -2.2 67,575 - Dshi_4135 0.65 -3.7 67,630 + Dshi_4135 0.69 +1.0 68,060 + +0.2 68,204 + Dshi_4136 0.14 +0.3 68,225 - Dshi_4136 0.16 -1.4 68,587 + Dshi_4136 0.47 +2.2 68,595 - Dshi_4136 0.47 +0.6 68,637 + Dshi_4136 0.51 -0.6 68,787 + Dshi_4136 0.64 -0.1 68,795 - Dshi_4136 0.64 -3.3 68,795 - Dshi_4136 0.64 -0.6 68,853 + Dshi_4136 0.69 -2.0 68,951 - Dshi_4136 0.77 +3.3 68,981 + Dshi_4136 0.80 -0.7 69,034 - Dshi_4136 0.84 -1.8
Or see this region's nucleotide sequence