Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4082 and Dshi_4083 overlap by 4 nucleotides Dshi_4083 and Dshi_4084 are separated by 122 nucleotides Dshi_4084 and Dshi_4085 overlap by 14 nucleotides
Dshi_4082: Dshi_4082 - Integrase catalytic region (RefSeq), at 16,978 to 18,471
_4082
Dshi_4083: Dshi_4083 - ATP-binding protein (RefSeq), at 18,468 to 19,292
_4083
Dshi_4084: Dshi_4084 - Resolvase domain (RefSeq), at 19,415 to 19,981
_4084
Dshi_4085: Dshi_4085 - WGR domain protein (RefSeq), at 19,968 to 20,222
_4085
Position (kb)
18
19
20 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 19.324 kb on + strand at 19.324 kb on + strand at 19.334 kb on + strand at 19.337 kb on + strand at 19.339 kb on + strand at 19.339 kb on + strand at 19.342 kb on - strand at 19.342 kb on - strand at 19.347 kb on - strand at 19.419 kb on + strand at 19.420 kb on + strand at 19.420 kb on + strand at 19.426 kb on - strand at 19.427 kb on - strand at 19.428 kb on - strand at 19.453 kb on + strand at 19.453 kb on + strand at 19.461 kb on - strand at 19.461 kb on - strand at 19.461 kb on - strand at 19.466 kb on - strand at 19.476 kb on - strand, within Dshi_4084 at 19.476 kb on - strand, within Dshi_4084 at 19.491 kb on + strand, within Dshi_4084 at 19.593 kb on + strand, within Dshi_4084 at 19.620 kb on - strand, within Dshi_4084 at 19.728 kb on + strand, within Dshi_4084 at 19.751 kb on - strand, within Dshi_4084 at 19.751 kb on - strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.796 kb on - strand, within Dshi_4084 at 19.796 kb on - strand, within Dshi_4084 at 19.798 kb on - strand, within Dshi_4084 at 19.798 kb on - strand, within Dshi_4084 at 19.833 kb on + strand, within Dshi_4084 at 19.841 kb on - strand, within Dshi_4084 at 19.875 kb on + strand, within Dshi_4084 at 19.885 kb on + strand, within Dshi_4084 at 19.894 kb on - strand, within Dshi_4084 at 19.900 kb on + strand, within Dshi_4084 at 19.900 kb on + strand, within Dshi_4084 at 19.908 kb on - strand, within Dshi_4084 at 19.908 kb on - strand, within Dshi_4084 at 19.961 kb on - strand at 20.012 kb on + strand, within Dshi_4085 at 20.012 kb on + strand, within Dshi_4085 at 20.069 kb on + strand, within Dshi_4085 at 20.069 kb on + strand, within Dshi_4085 at 20.077 kb on - strand, within Dshi_4085 at 20.080 kb on + strand, within Dshi_4085 at 20.121 kb on - strand, within Dshi_4085 at 20.121 kb on - strand, within Dshi_4085 at 20.136 kb on - strand, within Dshi_4085 at 20.145 kb on + strand, within Dshi_4085 at 20.153 kb on - strand, within Dshi_4085 at 20.160 kb on + strand, within Dshi_4085 at 20.180 kb on + strand, within Dshi_4085 at 20.193 kb on - strand, within Dshi_4085 at 20.203 kb on - strand at 20.213 kb on + strand at 20.218 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.228 kb on + strand at 20.228 kb on + strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.233 kb on + strand at 20.236 kb on - strand at 20.236 kb on - strand at 20.236 kb on - strand at 20.241 kb on - strand at 20.253 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 19,324 + +0.2 19,324 + +4.4 19,334 + +0.2 19,337 + +0.5 19,339 + -0.1 19,339 + +0.9 19,342 - -0.9 19,342 - +1.8 19,347 - +1.0 19,419 + -2.0 19,420 + +0.1 19,420 + +1.3 19,426 - +1.0 19,427 - +1.6 19,428 - -1.0 19,453 + +0.2 19,453 + +0.1 19,461 - -0.6 19,461 - +0.3 19,461 - -0.2 19,466 - +0.3 19,476 - Dshi_4084 0.11 -1.1 19,476 - Dshi_4084 0.11 -1.0 19,491 + Dshi_4084 0.13 -0.2 19,593 + Dshi_4084 0.31 -0.0 19,620 - Dshi_4084 0.36 -2.2 19,728 + Dshi_4084 0.55 +0.9 19,751 - Dshi_4084 0.59 -1.2 19,751 - Dshi_4084 0.59 -1.3 19,790 + Dshi_4084 0.66 +0.4 19,790 + Dshi_4084 0.66 -0.2 19,790 + Dshi_4084 0.66 +0.2 19,796 - Dshi_4084 0.67 +0.1 19,796 - Dshi_4084 0.67 -1.3 19,798 - Dshi_4084 0.68 -0.8 19,798 - Dshi_4084 0.68 +1.6 19,833 + Dshi_4084 0.74 -0.0 19,841 - Dshi_4084 0.75 +1.6 19,875 + Dshi_4084 0.81 +0.5 19,885 + Dshi_4084 0.83 +0.5 19,894 - Dshi_4084 0.84 -1.2 19,900 + Dshi_4084 0.86 -1.0 19,900 + Dshi_4084 0.86 +1.0 19,908 - Dshi_4084 0.87 -1.6 19,908 - Dshi_4084 0.87 +5.3 19,961 - -0.7 20,012 + Dshi_4085 0.17 -2.3 20,012 + Dshi_4085 0.17 +3.2 20,069 + Dshi_4085 0.40 -2.5 20,069 + Dshi_4085 0.40 -0.5 20,077 - Dshi_4085 0.43 +1.3 20,080 + Dshi_4085 0.44 +1.8 20,121 - Dshi_4085 0.60 +1.0 20,121 - Dshi_4085 0.60 +1.0 20,136 - Dshi_4085 0.66 +0.1 20,145 + Dshi_4085 0.69 +0.7 20,153 - Dshi_4085 0.73 +0.2 20,160 + Dshi_4085 0.75 +1.0 20,180 + Dshi_4085 0.83 +4.5 20,193 - Dshi_4085 0.88 +0.2 20,203 - +0.2 20,213 + -0.5 20,218 + -0.6 20,223 + -0.6 20,223 + -0.4 20,223 + -1.0 20,223 + +1.7 20,223 + +0.3 20,223 + -0.4 20,223 + -0.9 20,223 + +0.6 20,223 + +0.3 20,223 + +2.6 20,228 + +0.6 20,228 + -0.1 20,231 - +3.1 20,231 - +0.5 20,231 - -1.0 20,231 - +4.6 20,231 - -4.6 20,231 - -0.4 20,231 - -0.1 20,231 - +0.4 20,231 - +1.9 20,231 - -0.4 20,231 - -0.0 20,231 - +0.8 20,231 - +1.0 20,233 + +1.0 20,236 - +0.4 20,236 - -0.7 20,236 - -0.3 20,241 - +1.2 20,253 - +2.2
Or see this region's nucleotide sequence