Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3086 and Dshi_3087 are separated by 90 nucleotides Dshi_3087 and Dshi_3088 are separated by 12 nucleotides Dshi_3088 and Dshi_3089 are separated by 24 nucleotides Dshi_3089 and Dshi_3090 overlap by 4 nucleotides
Dshi_3086: Dshi_3086 - beta-lactamase domain protein (RefSeq), at 3,256,765 to 3,257,814
_3086
Dshi_3087: Dshi_3087 - aa3 type cytochrome c oxidase subunit IV (RefSeq), at 3,257,905 to 3,258,042
_3087
Dshi_3088: Dshi_3088 - hypothetical protein (RefSeq), at 3,258,055 to 3,258,663
_3088
Dshi_3089: Dshi_3089 - molybdenum cofactor biosynthesis protein B (RefSeq), at 3,258,688 to 3,259,230
_3089
Dshi_3090: Dshi_3090 - phage SPO1 DNA polymerase-related protein (RefSeq), at 3,259,227 to 3,260,024
_3090
Position (kb)
3258
3259 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3257.064 kb on + strand, within Dshi_3086 at 3257.089 kb on - strand, within Dshi_3086 at 3257.459 kb on - strand, within Dshi_3086 at 3257.535 kb on - strand, within Dshi_3086 at 3257.591 kb on + strand, within Dshi_3086 at 3257.712 kb on + strand at 3257.830 kb on + strand at 3257.906 kb on + strand at 3257.957 kb on - strand, within Dshi_3087 at 3258.085 kb on - strand at 3258.197 kb on - strand, within Dshi_3088 at 3258.209 kb on + strand, within Dshi_3088 at 3258.221 kb on - strand, within Dshi_3088 at 3258.339 kb on + strand, within Dshi_3088 at 3258.340 kb on - strand, within Dshi_3088 at 3258.406 kb on - strand, within Dshi_3088 at 3258.574 kb on - strand, within Dshi_3088 at 3258.618 kb on + strand at 3258.643 kb on + strand at 3258.657 kb on + strand at 3258.857 kb on + strand, within Dshi_3089 at 3258.969 kb on + strand, within Dshi_3089 at 3259.045 kb on - strand, within Dshi_3089 at 3259.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 3,257,064 + Dshi_3086 0.28 -0.3 3,257,089 - Dshi_3086 0.31 +0.3 3,257,459 - Dshi_3086 0.66 -0.7 3,257,535 - Dshi_3086 0.73 -2.4 3,257,591 + Dshi_3086 0.79 -2.1 3,257,712 + -2.8 3,257,830 + -3.8 3,257,906 + -1.4 3,257,957 - Dshi_3087 0.38 -0.7 3,258,085 - -0.3 3,258,197 - Dshi_3088 0.23 +0.2 3,258,209 + Dshi_3088 0.25 +0.5 3,258,221 - Dshi_3088 0.27 -0.1 3,258,339 + Dshi_3088 0.47 +0.6 3,258,340 - Dshi_3088 0.47 +0.9 3,258,406 - Dshi_3088 0.58 +0.4 3,258,574 - Dshi_3088 0.85 -1.6 3,258,618 + +0.2 3,258,643 + -1.1 3,258,657 + -0.5 3,258,857 + Dshi_3089 0.31 -0.8 3,258,969 + Dshi_3089 0.52 +1.9 3,259,045 - Dshi_3089 0.66 +1.2 3,259,212 - -0.1
Or see this region's nucleotide sequence