Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2627 and Dshi_2628 overlap by 4 nucleotides Dshi_2628 and Dshi_2629 are separated by 27 nucleotides Dshi_2629 and Dshi_2630 are separated by 3 nucleotides Dshi_2630 and Dshi_2631 are separated by 131 nucleotides
Dshi_2627: Dshi_2627 - nitroreductase (RefSeq), at 2,785,328 to 2,785,978
_2627
Dshi_2628: Dshi_2628 - hypothetical protein (RefSeq), at 2,785,975 to 2,786,685
_2628
Dshi_2629: Dshi_2629 - protein of unknown function DUF1467 (RefSeq), at 2,786,713 to 2,787,000
_2629
Dshi_2630: Dshi_2630 - Methylmalonyl-CoA epimerase (RefSeq), at 2,787,004 to 2,787,408
_2630
Dshi_2631: Dshi_2631 - response regulator receiver protein (RefSeq), at 2,787,540 to 2,788,244
_2631
Position (kb)
2786
2787
2788 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2785.821 kb on + strand, within Dshi_2627 at 2785.931 kb on - strand at 2786.043 kb on + strand at 2786.375 kb on - strand, within Dshi_2628 at 2786.455 kb on + strand, within Dshi_2628 at 2786.665 kb on - strand at 2786.743 kb on - strand, within Dshi_2629 at 2786.984 kb on - strand at 2787.002 kb on + strand at 2787.002 kb on + strand at 2787.010 kb on - strand at 2787.425 kb on - strand at 2787.451 kb on + strand at 2787.544 kb on - strand at 2787.765 kb on - strand, within Dshi_2631 at 2787.863 kb on + strand, within Dshi_2631 at 2787.920 kb on + strand, within Dshi_2631
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 2,785,821 + Dshi_2627 0.76 +0.6 2,785,931 - -2.4 2,786,043 + -0.4 2,786,375 - Dshi_2628 0.56 -0.4 2,786,455 + Dshi_2628 0.68 +0.6 2,786,665 - -3.5 2,786,743 - Dshi_2629 0.10 +2.2 2,786,984 - -2.1 2,787,002 + -1.0 2,787,002 + -2.6 2,787,010 - -1.4 2,787,425 - +0.6 2,787,451 + -0.2 2,787,544 - -3.8 2,787,765 - Dshi_2631 0.32 -1.0 2,787,863 + Dshi_2631 0.46 -1.6 2,787,920 + Dshi_2631 0.54 -1.0
Or see this region's nucleotide sequence