Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2546 and Dshi_2547 are separated by 91 nucleotides Dshi_2547 and Dshi_2548 are separated by 16 nucleotides Dshi_2548 and Dshi_2549 are separated by 3 nucleotides
Dshi_2546: Dshi_2546 - Malate/L-lactate dehydrogenase (RefSeq), at 2,702,195 to 2,703,250
_2546
Dshi_2547: Dshi_2547 - hypothetical protein (RefSeq), at 2,703,342 to 2,703,722
_2547
Dshi_2548: Dshi_2548 - hypothetical protein (RefSeq), at 2,703,739 to 2,707,950
_2548
Dshi_2549: Dshi_2549 - cell surface receptor IPT/TIG domain protein (RefSeq), at 2,707,954 to 2,709,441
_2549
Position (kb)
2703
2704
2705
2706
2707
2708 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2702.758 kb on + strand, within Dshi_2546 at 2702.766 kb on - strand, within Dshi_2546 at 2702.864 kb on - strand, within Dshi_2546 at 2702.874 kb on - strand, within Dshi_2546 at 2702.886 kb on - strand, within Dshi_2546 at 2703.041 kb on - strand, within Dshi_2546 at 2703.046 kb on - strand, within Dshi_2546 at 2703.090 kb on + strand, within Dshi_2546 at 2703.106 kb on + strand, within Dshi_2546 at 2703.106 kb on + strand, within Dshi_2546 at 2703.147 kb on - strand at 2703.177 kb on + strand at 2703.249 kb on + strand at 2703.332 kb on - strand at 2703.350 kb on - strand at 2703.373 kb on + strand at 2703.377 kb on + strand at 2703.385 kb on - strand, within Dshi_2547 at 2703.415 kb on - strand, within Dshi_2547 at 2703.429 kb on - strand, within Dshi_2547 at 2703.434 kb on - strand, within Dshi_2547 at 2703.436 kb on + strand, within Dshi_2547 at 2703.436 kb on + strand, within Dshi_2547 at 2703.451 kb on + strand, within Dshi_2547 at 2703.451 kb on + strand, within Dshi_2547 at 2703.454 kb on - strand, within Dshi_2547 at 2703.459 kb on - strand, within Dshi_2547 at 2703.479 kb on - strand, within Dshi_2547 at 2703.578 kb on + strand, within Dshi_2547 at 2703.586 kb on - strand, within Dshi_2547 at 2703.586 kb on - strand, within Dshi_2547 at 2703.601 kb on - strand, within Dshi_2547 at 2703.663 kb on + strand, within Dshi_2547 at 2703.671 kb on - strand, within Dshi_2547 at 2703.731 kb on - strand at 2703.742 kb on + strand at 2703.742 kb on + strand at 2703.750 kb on - strand at 2703.804 kb on + strand at 2703.817 kb on - strand at 2703.844 kb on + strand at 2703.912 kb on - strand at 2703.926 kb on + strand at 2703.926 kb on + strand at 2703.926 kb on + strand at 2703.934 kb on - strand at 2703.934 kb on - strand at 2703.934 kb on - strand at 2703.950 kb on + strand at 2703.992 kb on + strand at 2704.122 kb on - strand at 2704.139 kb on - strand at 2704.147 kb on - strand at 2704.147 kb on - strand at 2704.163 kb on + strand, within Dshi_2548 at 2704.215 kb on - strand, within Dshi_2548 at 2704.295 kb on + strand, within Dshi_2548 at 2704.301 kb on + strand, within Dshi_2548 at 2704.302 kb on + strand, within Dshi_2548 at 2704.302 kb on + strand, within Dshi_2548 at 2704.303 kb on - strand, within Dshi_2548 at 2704.395 kb on - strand, within Dshi_2548 at 2704.442 kb on - strand, within Dshi_2548 at 2704.452 kb on + strand, within Dshi_2548 at 2704.452 kb on + strand, within Dshi_2548 at 2704.452 kb on + strand, within Dshi_2548 at 2704.452 kb on + strand, within Dshi_2548 at 2704.460 kb on - strand, within Dshi_2548 at 2704.460 kb on - strand, within Dshi_2548 at 2704.460 kb on - strand, within Dshi_2548 at 2704.465 kb on + strand, within Dshi_2548 at 2704.500 kb on - strand, within Dshi_2548 at 2704.511 kb on + strand, within Dshi_2548 at 2704.572 kb on + strand, within Dshi_2548 at 2704.720 kb on - strand, within Dshi_2548 at 2704.727 kb on - strand, within Dshi_2548 at 2704.727 kb on - strand, within Dshi_2548 at 2704.797 kb on + strand, within Dshi_2548 at 2704.815 kb on + strand, within Dshi_2548 at 2704.820 kb on + strand, within Dshi_2548 at 2704.824 kb on + strand, within Dshi_2548 at 2704.869 kb on + strand, within Dshi_2548 at 2704.906 kb on + strand, within Dshi_2548 at 2705.018 kb on - strand, within Dshi_2548 at 2705.073 kb on - strand, within Dshi_2548 at 2705.116 kb on - strand, within Dshi_2548 at 2705.121 kb on + strand, within Dshi_2548 at 2705.128 kb on - strand, within Dshi_2548 at 2705.128 kb on - strand, within Dshi_2548 at 2705.151 kb on - strand, within Dshi_2548 at 2705.160 kb on - strand, within Dshi_2548 at 2705.301 kb on + strand, within Dshi_2548 at 2705.304 kb on + strand, within Dshi_2548 at 2705.309 kb on - strand, within Dshi_2548 at 2705.312 kb on - strand, within Dshi_2548 at 2705.413 kb on - strand, within Dshi_2548 at 2705.421 kb on - strand, within Dshi_2548 at 2705.448 kb on - strand, within Dshi_2548 at 2705.448 kb on - strand, within Dshi_2548 at 2705.518 kb on - strand, within Dshi_2548 at 2705.524 kb on + strand, within Dshi_2548 at 2705.584 kb on - strand, within Dshi_2548 at 2705.750 kb on + strand, within Dshi_2548 at 2705.750 kb on + strand, within Dshi_2548 at 2705.750 kb on + strand, within Dshi_2548 at 2705.758 kb on - strand, within Dshi_2548 at 2705.758 kb on - strand, within Dshi_2548 at 2705.758 kb on - strand, within Dshi_2548 at 2705.762 kb on - strand, within Dshi_2548 at 2705.772 kb on + strand, within Dshi_2548 at 2705.814 kb on - strand, within Dshi_2548 at 2705.827 kb on + strand, within Dshi_2548 at 2705.874 kb on - strand, within Dshi_2548 at 2705.933 kb on + strand, within Dshi_2548 at 2705.941 kb on - strand, within Dshi_2548 at 2705.941 kb on - strand, within Dshi_2548 at 2706.268 kb on + strand, within Dshi_2548 at 2706.268 kb on + strand, within Dshi_2548 at 2706.276 kb on - strand, within Dshi_2548 at 2706.301 kb on + strand, within Dshi_2548 at 2706.338 kb on - strand, within Dshi_2548 at 2706.359 kb on + strand, within Dshi_2548 at 2706.366 kb on - strand, within Dshi_2548 at 2706.381 kb on + strand, within Dshi_2548 at 2706.442 kb on - strand, within Dshi_2548 at 2706.510 kb on - strand, within Dshi_2548 at 2706.526 kb on + strand, within Dshi_2548 at 2706.526 kb on + strand, within Dshi_2548 at 2706.627 kb on + strand, within Dshi_2548 at 2706.635 kb on - strand, within Dshi_2548 at 2706.646 kb on - strand, within Dshi_2548 at 2706.646 kb on - strand, within Dshi_2548 at 2706.701 kb on - strand, within Dshi_2548 at 2706.732 kb on + strand, within Dshi_2548 at 2706.735 kb on - strand, within Dshi_2548 at 2706.771 kb on + strand, within Dshi_2548 at 2706.781 kb on - strand, within Dshi_2548 at 2706.785 kb on - strand, within Dshi_2548 at 2706.794 kb on + strand, within Dshi_2548 at 2706.802 kb on - strand, within Dshi_2548 at 2706.839 kb on + strand, within Dshi_2548 at 2706.872 kb on - strand, within Dshi_2548 at 2707.057 kb on - strand, within Dshi_2548 at 2707.071 kb on + strand, within Dshi_2548 at 2707.113 kb on + strand, within Dshi_2548 at 2707.217 kb on - strand, within Dshi_2548 at 2707.351 kb on + strand, within Dshi_2548 at 2707.352 kb on - strand, within Dshi_2548 at 2707.457 kb on - strand, within Dshi_2548 at 2707.462 kb on + strand, within Dshi_2548 at 2707.499 kb on - strand, within Dshi_2548 at 2707.523 kb on - strand, within Dshi_2548 at 2707.562 kb on + strand at 2707.570 kb on - strand at 2707.616 kb on + strand at 2707.616 kb on + strand at 2707.727 kb on + strand at 2707.824 kb on + strand at 2707.850 kb on + strand at 2707.857 kb on + strand at 2707.866 kb on - strand at 2707.866 kb on - strand at 2707.869 kb on + strand at 2707.885 kb on - strand at 2707.942 kb on + strand at 2708.140 kb on + strand, within Dshi_2549 at 2708.149 kb on + strand, within Dshi_2549 at 2708.150 kb on - strand, within Dshi_2549 at 2708.233 kb on + strand, within Dshi_2549 at 2708.319 kb on - strand, within Dshi_2549 at 2708.340 kb on - strand, within Dshi_2549 at 2708.516 kb on + strand, within Dshi_2549 at 2708.536 kb on + strand, within Dshi_2549 at 2708.550 kb on - strand, within Dshi_2549 at 2708.673 kb on - strand, within Dshi_2549 at 2708.712 kb on + strand, within Dshi_2549 at 2708.712 kb on + strand, within Dshi_2549 at 2708.719 kb on + strand, within Dshi_2549 at 2708.720 kb on - strand, within Dshi_2549 at 2708.768 kb on - strand, within Dshi_2549 at 2708.832 kb on + strand, within Dshi_2549 at 2708.832 kb on + strand, within Dshi_2549 at 2708.837 kb on + strand, within Dshi_2549 at 2708.868 kb on - strand, within Dshi_2549 at 2708.903 kb on + strand, within Dshi_2549 at 2708.944 kb on - strand, within Dshi_2549
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 2,702,758 + Dshi_2546 0.53 -1.0 2,702,766 - Dshi_2546 0.54 +1.0 2,702,864 - Dshi_2546 0.63 +0.1 2,702,874 - Dshi_2546 0.64 +1.2 2,702,886 - Dshi_2546 0.65 -0.6 2,703,041 - Dshi_2546 0.80 -0.5 2,703,046 - Dshi_2546 0.81 -0.9 2,703,090 + Dshi_2546 0.85 -0.9 2,703,106 + Dshi_2546 0.86 +1.0 2,703,106 + Dshi_2546 0.86 -0.3 2,703,147 - -0.4 2,703,177 + -0.1 2,703,249 + +0.8 2,703,332 - +2.9 2,703,350 - -0.4 2,703,373 + +0.9 2,703,377 + -0.9 2,703,385 - Dshi_2547 0.11 -1.4 2,703,415 - Dshi_2547 0.19 +0.5 2,703,429 - Dshi_2547 0.23 +0.0 2,703,434 - Dshi_2547 0.24 +2.7 2,703,436 + Dshi_2547 0.25 +1.5 2,703,436 + Dshi_2547 0.25 -1.0 2,703,451 + Dshi_2547 0.29 +1.3 2,703,451 + Dshi_2547 0.29 +0.6 2,703,454 - Dshi_2547 0.29 -0.6 2,703,459 - Dshi_2547 0.31 +0.4 2,703,479 - Dshi_2547 0.36 -3.1 2,703,578 + Dshi_2547 0.62 +0.1 2,703,586 - Dshi_2547 0.64 -0.2 2,703,586 - Dshi_2547 0.64 -1.7 2,703,601 - Dshi_2547 0.68 +0.4 2,703,663 + Dshi_2547 0.84 +0.6 2,703,671 - Dshi_2547 0.86 +0.2 2,703,731 - -0.9 2,703,742 + -0.3 2,703,742 + +1.7 2,703,750 - +0.6 2,703,804 + +0.1 2,703,817 - +0.4 2,703,844 + -0.9 2,703,912 - -0.0 2,703,926 + +0.6 2,703,926 + +1.0 2,703,926 + +0.2 2,703,934 - -0.4 2,703,934 - +0.3 2,703,934 - +1.4 2,703,950 + -1.0 2,703,992 + +0.9 2,704,122 - -0.4 2,704,139 - -0.9 2,704,147 - +0.2 2,704,147 - +1.1 2,704,163 + Dshi_2548 0.10 +0.6 2,704,215 - Dshi_2548 0.11 +0.2 2,704,295 + Dshi_2548 0.13 +0.0 2,704,301 + Dshi_2548 0.13 +0.6 2,704,302 + Dshi_2548 0.13 -1.0 2,704,302 + Dshi_2548 0.13 -0.4 2,704,303 - Dshi_2548 0.13 +2.5 2,704,395 - Dshi_2548 0.16 +0.0 2,704,442 - Dshi_2548 0.17 -2.2 2,704,452 + Dshi_2548 0.17 +0.1 2,704,452 + Dshi_2548 0.17 -1.8 2,704,452 + Dshi_2548 0.17 -0.5 2,704,452 + Dshi_2548 0.17 +0.1 2,704,460 - Dshi_2548 0.17 +0.3 2,704,460 - Dshi_2548 0.17 +2.1 2,704,460 - Dshi_2548 0.17 -0.4 2,704,465 + Dshi_2548 0.17 +0.6 2,704,500 - Dshi_2548 0.18 +0.8 2,704,511 + Dshi_2548 0.18 +0.4 2,704,572 + Dshi_2548 0.20 -0.9 2,704,720 - Dshi_2548 0.23 -0.5 2,704,727 - Dshi_2548 0.23 +0.1 2,704,727 - Dshi_2548 0.23 +0.1 2,704,797 + Dshi_2548 0.25 +1.0 2,704,815 + Dshi_2548 0.26 +0.6 2,704,820 + Dshi_2548 0.26 -0.8 2,704,824 + Dshi_2548 0.26 -1.7 2,704,869 + Dshi_2548 0.27 +0.2 2,704,906 + Dshi_2548 0.28 +0.5 2,705,018 - Dshi_2548 0.30 +1.6 2,705,073 - Dshi_2548 0.32 -4.4 2,705,116 - Dshi_2548 0.33 +1.6 2,705,121 + Dshi_2548 0.33 +0.1 2,705,128 - Dshi_2548 0.33 +0.6 2,705,128 - Dshi_2548 0.33 -3.3 2,705,151 - Dshi_2548 0.34 -0.2 2,705,160 - Dshi_2548 0.34 -3.7 2,705,301 + Dshi_2548 0.37 -1.0 2,705,304 + Dshi_2548 0.37 -0.9 2,705,309 - Dshi_2548 0.37 -0.9 2,705,312 - Dshi_2548 0.37 +0.3 2,705,413 - Dshi_2548 0.40 -3.6 2,705,421 - Dshi_2548 0.40 -1.3 2,705,448 - Dshi_2548 0.41 +0.6 2,705,448 - Dshi_2548 0.41 +1.3 2,705,518 - Dshi_2548 0.42 -2.6 2,705,524 + Dshi_2548 0.42 -0.3 2,705,584 - Dshi_2548 0.44 -0.2 2,705,750 + Dshi_2548 0.48 -2.0 2,705,750 + Dshi_2548 0.48 -1.6 2,705,750 + Dshi_2548 0.48 -0.6 2,705,758 - Dshi_2548 0.48 +2.9 2,705,758 - Dshi_2548 0.48 -0.6 2,705,758 - Dshi_2548 0.48 +0.3 2,705,762 - Dshi_2548 0.48 -1.5 2,705,772 + Dshi_2548 0.48 +1.6 2,705,814 - Dshi_2548 0.49 +0.9 2,705,827 + Dshi_2548 0.50 +1.1 2,705,874 - Dshi_2548 0.51 +0.4 2,705,933 + Dshi_2548 0.52 -0.5 2,705,941 - Dshi_2548 0.52 -0.2 2,705,941 - Dshi_2548 0.52 +0.8 2,706,268 + Dshi_2548 0.60 +0.1 2,706,268 + Dshi_2548 0.60 +0.7 2,706,276 - Dshi_2548 0.60 -0.0 2,706,301 + Dshi_2548 0.61 -0.4 2,706,338 - Dshi_2548 0.62 -0.4 2,706,359 + Dshi_2548 0.62 -1.0 2,706,366 - Dshi_2548 0.62 -2.3 2,706,381 + Dshi_2548 0.63 +1.1 2,706,442 - Dshi_2548 0.64 -1.1 2,706,510 - Dshi_2548 0.66 +0.8 2,706,526 + Dshi_2548 0.66 +1.6 2,706,526 + Dshi_2548 0.66 -0.0 2,706,627 + Dshi_2548 0.69 -0.1 2,706,635 - Dshi_2548 0.69 +0.5 2,706,646 - Dshi_2548 0.69 +1.3 2,706,646 - Dshi_2548 0.69 +0.3 2,706,701 - Dshi_2548 0.70 +1.9 2,706,732 + Dshi_2548 0.71 +1.1 2,706,735 - Dshi_2548 0.71 +0.6 2,706,771 + Dshi_2548 0.72 -0.6 2,706,781 - Dshi_2548 0.72 -0.3 2,706,785 - Dshi_2548 0.72 +0.4 2,706,794 + Dshi_2548 0.73 -1.3 2,706,802 - Dshi_2548 0.73 -0.3 2,706,839 + Dshi_2548 0.74 -1.3 2,706,872 - Dshi_2548 0.74 -0.2 2,707,057 - Dshi_2548 0.79 -1.7 2,707,071 + Dshi_2548 0.79 -0.2 2,707,113 + Dshi_2548 0.80 -0.8 2,707,217 - Dshi_2548 0.83 -1.0 2,707,351 + Dshi_2548 0.86 +0.8 2,707,352 - Dshi_2548 0.86 -0.3 2,707,457 - Dshi_2548 0.88 +0.9 2,707,462 + Dshi_2548 0.88 -0.4 2,707,499 - Dshi_2548 0.89 +0.6 2,707,523 - Dshi_2548 0.90 +0.3 2,707,562 + -1.3 2,707,570 - +1.6 2,707,616 + -2.2 2,707,616 + -0.2 2,707,727 + +0.6 2,707,824 + -0.1 2,707,850 + +0.2 2,707,857 + -0.2 2,707,866 - -0.5 2,707,866 - +0.6 2,707,869 + -1.1 2,707,885 - +3.7 2,707,942 + -0.7 2,708,140 + Dshi_2549 0.12 -2.9 2,708,149 + Dshi_2549 0.13 -0.2 2,708,150 - Dshi_2549 0.13 +0.4 2,708,233 + Dshi_2549 0.19 -1.6 2,708,319 - Dshi_2549 0.25 -1.2 2,708,340 - Dshi_2549 0.26 -0.0 2,708,516 + Dshi_2549 0.38 -0.3 2,708,536 + Dshi_2549 0.39 +1.3 2,708,550 - Dshi_2549 0.40 +0.4 2,708,673 - Dshi_2549 0.48 +0.2 2,708,712 + Dshi_2549 0.51 -1.4 2,708,712 + Dshi_2549 0.51 +0.2 2,708,719 + Dshi_2549 0.51 +0.2 2,708,720 - Dshi_2549 0.51 -0.7 2,708,768 - Dshi_2549 0.55 +0.6 2,708,832 + Dshi_2549 0.59 +0.8 2,708,832 + Dshi_2549 0.59 -0.9 2,708,837 + Dshi_2549 0.59 +1.0 2,708,868 - Dshi_2549 0.61 -0.9 2,708,903 + Dshi_2549 0.64 -1.6 2,708,944 - Dshi_2549 0.67 -0.2
Or see this region's nucleotide sequence