Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2532 and Dshi_2533 are separated by 337 nucleotides Dshi_2533 and Dshi_2534 are separated by 14 nucleotides Dshi_2534 and Dshi_2535 are separated by 188 nucleotides Dshi_2535 and Dshi_2536 are separated by 75 nucleotides Dshi_2536 and Dshi_2537 are separated by 2 nucleotides
Dshi_2532: Dshi_2532 - twin-arginine translocation pathway signal (RefSeq), at 2,692,195 to 2,692,497
_2532
Dshi_2533: Dshi_2533 - hypothetical protein (RefSeq), at 2,692,835 to 2,693,023
_2533
Dshi_2534: Dshi_2534 - integral membrane protein (RefSeq), at 2,693,038 to 2,693,469
_2534
Dshi_2535: Dshi_2535 - permease (RefSeq), at 2,693,658 to 2,693,837
_2535
Dshi_2536: Dshi_2536 - protein of unknown function DUF81 (RefSeq), at 2,693,913 to 2,694,260
_2536
Dshi_2537: Dshi_2537 - 4,5-dihydroxyphthalate decarboxylase (RefSeq), at 2,694,263 to 2,695,291
_2537
Position (kb)
2693
2694 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2692.071 kb on + strand at 2692.071 kb on + strand at 2692.102 kb on + strand at 2692.121 kb on + strand at 2692.127 kb on + strand at 2692.156 kb on + strand at 2692.188 kb on + strand at 2692.219 kb on - strand at 2692.249 kb on + strand, within Dshi_2532 at 2692.305 kb on - strand, within Dshi_2532 at 2692.325 kb on + strand, within Dshi_2532 at 2692.328 kb on - strand, within Dshi_2532 at 2692.366 kb on - strand, within Dshi_2532 at 2692.385 kb on - strand, within Dshi_2532 at 2692.408 kb on + strand, within Dshi_2532 at 2692.413 kb on + strand, within Dshi_2532 at 2692.441 kb on + strand, within Dshi_2532 at 2692.451 kb on + strand, within Dshi_2532 at 2692.453 kb on - strand, within Dshi_2532 at 2692.499 kb on + strand at 2692.507 kb on - strand at 2692.507 kb on - strand at 2692.519 kb on + strand at 2692.519 kb on + strand at 2692.529 kb on + strand at 2692.597 kb on - strand at 2692.687 kb on + strand at 2692.705 kb on - strand at 2692.720 kb on + strand at 2692.725 kb on + strand at 2692.740 kb on - strand at 2692.758 kb on - strand at 2692.791 kb on + strand at 2692.796 kb on + strand at 2692.796 kb on + strand at 2692.814 kb on - strand at 2692.842 kb on + strand at 2692.906 kb on - strand, within Dshi_2533 at 2692.986 kb on - strand, within Dshi_2533 at 2693.013 kb on - strand at 2693.066 kb on + strand at 2693.071 kb on + strand at 2693.071 kb on + strand at 2693.071 kb on + strand at 2693.076 kb on + strand at 2693.079 kb on - strand at 2693.084 kb on - strand, within Dshi_2534 at 2693.101 kb on - strand, within Dshi_2534 at 2693.126 kb on + strand, within Dshi_2534 at 2693.137 kb on + strand, within Dshi_2534 at 2693.145 kb on - strand, within Dshi_2534 at 2693.145 kb on - strand, within Dshi_2534 at 2693.220 kb on - strand, within Dshi_2534 at 2693.239 kb on + strand, within Dshi_2534 at 2693.241 kb on - strand, within Dshi_2534 at 2693.277 kb on + strand, within Dshi_2534 at 2693.324 kb on + strand, within Dshi_2534 at 2693.413 kb on + strand, within Dshi_2534 at 2693.423 kb on + strand, within Dshi_2534 at 2693.431 kb on + strand at 2693.431 kb on - strand at 2693.454 kb on - strand at 2693.456 kb on + strand at 2693.470 kb on + strand at 2693.470 kb on + strand at 2693.473 kb on - strand at 2693.529 kb on + strand at 2693.529 kb on + strand at 2693.537 kb on - strand at 2693.537 kb on - strand at 2693.537 kb on - strand at 2693.650 kb on - strand at 2693.672 kb on + strand at 2693.695 kb on + strand, within Dshi_2535 at 2693.730 kb on + strand, within Dshi_2535 at 2693.740 kb on - strand, within Dshi_2535 at 2693.770 kb on - strand, within Dshi_2535 at 2693.783 kb on + strand, within Dshi_2535 at 2693.793 kb on + strand, within Dshi_2535 at 2693.905 kb on - strand at 2693.918 kb on + strand at 2693.918 kb on + strand at 2693.918 kb on + strand at 2693.918 kb on + strand at 2693.936 kb on - strand at 2694.090 kb on + strand, within Dshi_2536 at 2694.096 kb on - strand, within Dshi_2536 at 2694.212 kb on + strand, within Dshi_2536 at 2694.212 kb on + strand, within Dshi_2536 at 2694.212 kb on + strand, within Dshi_2536 at 2694.220 kb on - strand, within Dshi_2536 at 2694.231 kb on + strand at 2694.231 kb on + strand at 2694.231 kb on + strand at 2694.231 kb on + strand at 2694.231 kb on + strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.239 kb on - strand at 2694.254 kb on - strand at 2694.274 kb on + strand at 2694.432 kb on + strand, within Dshi_2537 at 2694.438 kb on + strand, within Dshi_2537
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 2,692,071 + -0.1 2,692,071 + +0.3 2,692,102 + -0.8 2,692,121 + +0.2 2,692,127 + -2.8 2,692,156 + -0.9 2,692,188 + -1.4 2,692,219 - +0.2 2,692,249 + Dshi_2532 0.18 -2.4 2,692,305 - Dshi_2532 0.36 -0.7 2,692,325 + Dshi_2532 0.43 -1.0 2,692,328 - Dshi_2532 0.44 +2.4 2,692,366 - Dshi_2532 0.56 -0.6 2,692,385 - Dshi_2532 0.63 +0.8 2,692,408 + Dshi_2532 0.70 +0.4 2,692,413 + Dshi_2532 0.72 +0.7 2,692,441 + Dshi_2532 0.81 +0.4 2,692,451 + Dshi_2532 0.84 -1.8 2,692,453 - Dshi_2532 0.85 +0.3 2,692,499 + -0.3 2,692,507 - +0.4 2,692,507 - -2.0 2,692,519 + +0.2 2,692,519 + -0.3 2,692,529 + -0.6 2,692,597 - -1.8 2,692,687 + -1.2 2,692,705 - +0.3 2,692,720 + +2.4 2,692,725 + +0.6 2,692,740 - -0.1 2,692,758 - -0.8 2,692,791 + +0.2 2,692,796 + +0.4 2,692,796 + +1.1 2,692,814 - +0.9 2,692,842 + -0.0 2,692,906 - Dshi_2533 0.38 +0.6 2,692,986 - Dshi_2533 0.80 +0.1 2,693,013 - +0.3 2,693,066 + +0.2 2,693,071 + +0.8 2,693,071 + -0.4 2,693,071 + -0.3 2,693,076 + -0.0 2,693,079 - -0.6 2,693,084 - Dshi_2534 0.11 +0.1 2,693,101 - Dshi_2534 0.15 -0.4 2,693,126 + Dshi_2534 0.20 -0.3 2,693,137 + Dshi_2534 0.23 +1.0 2,693,145 - Dshi_2534 0.25 +0.8 2,693,145 - Dshi_2534 0.25 +3.1 2,693,220 - Dshi_2534 0.42 +1.4 2,693,239 + Dshi_2534 0.47 -0.3 2,693,241 - Dshi_2534 0.47 -3.0 2,693,277 + Dshi_2534 0.55 +0.6 2,693,324 + Dshi_2534 0.66 +1.2 2,693,413 + Dshi_2534 0.87 -0.6 2,693,423 + Dshi_2534 0.89 -4.0 2,693,431 + +0.2 2,693,431 - -0.1 2,693,454 - -0.3 2,693,456 + +0.3 2,693,470 + +0.5 2,693,470 + -0.8 2,693,473 - -0.7 2,693,529 + +1.4 2,693,529 + +0.4 2,693,537 - -0.8 2,693,537 - +1.4 2,693,537 - +0.2 2,693,650 - +3.1 2,693,672 + +1.1 2,693,695 + Dshi_2535 0.21 -1.1 2,693,730 + Dshi_2535 0.40 +0.8 2,693,740 - Dshi_2535 0.46 +2.9 2,693,770 - Dshi_2535 0.62 +0.9 2,693,783 + Dshi_2535 0.69 -0.5 2,693,793 + Dshi_2535 0.75 -1.0 2,693,905 - +0.0 2,693,918 + -0.8 2,693,918 + -1.0 2,693,918 + -1.4 2,693,918 + -1.7 2,693,936 - +0.3 2,694,090 + Dshi_2536 0.51 -1.2 2,694,096 - Dshi_2536 0.53 +0.2 2,694,212 + Dshi_2536 0.86 -0.6 2,694,212 + Dshi_2536 0.86 +2.6 2,694,212 + Dshi_2536 0.86 +0.8 2,694,220 - Dshi_2536 0.88 +0.6 2,694,231 + -0.8 2,694,231 + +0.1 2,694,231 + +0.6 2,694,231 + -0.3 2,694,231 + -0.9 2,694,239 - +0.1 2,694,239 - -0.4 2,694,239 - -0.1 2,694,239 - +0.4 2,694,239 - -0.5 2,694,239 - +0.7 2,694,239 - -1.2 2,694,239 - +0.6 2,694,239 - +0.8 2,694,254 - +0.0 2,694,274 + +1.5 2,694,432 + Dshi_2537 0.16 -0.4 2,694,438 + Dshi_2537 0.17 +0.5
Or see this region's nucleotide sequence