Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2258 and Dshi_2259 are separated by 49 nucleotides Dshi_2259 and Dshi_2260 are separated by 210 nucleotides Dshi_2260 and Dshi_2261 are separated by 51 nucleotides
Dshi_2258: Dshi_2258 - protein of unknown function UPF0047 (RefSeq), at 2,379,941 to 2,380,360
_2258
Dshi_2259: Dshi_2259 - hypothetical protein (RefSeq), at 2,380,410 to 2,380,916
_2259
Dshi_2260: Dshi_2260 - hypothetical protein (RefSeq), at 2,381,127 to 2,381,549
_2260
Dshi_2261: Dshi_2261 - RNA polymerase, sigma 70 subunit, RpoD family (RefSeq), at 2,381,601 to 2,383,595
_2261
Position (kb)
2381
2382 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2380.233 kb on - strand, within Dshi_2258 at 2380.235 kb on + strand, within Dshi_2258 at 2380.415 kb on - strand at 2380.493 kb on + strand, within Dshi_2259 at 2380.508 kb on - strand, within Dshi_2259 at 2380.546 kb on + strand, within Dshi_2259 at 2380.739 kb on - strand, within Dshi_2259 at 2380.742 kb on + strand, within Dshi_2259 at 2380.793 kb on - strand, within Dshi_2259 at 2380.798 kb on - strand, within Dshi_2259 at 2380.799 kb on + strand, within Dshi_2259 at 2380.964 kb on - strand at 2380.990 kb on + strand at 2380.998 kb on - strand at 2381.014 kb on + strand at 2381.017 kb on + strand at 2381.025 kb on - strand at 2381.025 kb on - strand at 2381.032 kb on - strand at 2381.033 kb on - strand at 2381.048 kb on + strand at 2381.093 kb on + strand at 2381.145 kb on + strand at 2381.317 kb on + strand, within Dshi_2260 at 2381.381 kb on + strand, within Dshi_2260 at 2381.389 kb on - strand at 2381.399 kb on - strand, within Dshi_2260 at 2381.399 kb on - strand, within Dshi_2260 at 2381.576 kb on + strand at 2381.596 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 2,380,233 - Dshi_2258 0.70 -0.1 2,380,235 + Dshi_2258 0.70 -1.3 2,380,415 - -0.2 2,380,493 + Dshi_2259 0.16 +0.1 2,380,508 - Dshi_2259 0.19 -1.2 2,380,546 + Dshi_2259 0.27 +0.2 2,380,739 - Dshi_2259 0.65 -3.1 2,380,742 + Dshi_2259 0.65 -4.2 2,380,793 - Dshi_2259 0.76 -2.0 2,380,798 - Dshi_2259 0.77 -2.1 2,380,799 + Dshi_2259 0.77 -4.2 2,380,964 - -1.4 2,380,990 + -0.4 2,380,998 - -0.5 2,381,014 + -1.5 2,381,017 + -2.0 2,381,025 - -2.3 2,381,025 - -3.1 2,381,032 - +0.4 2,381,033 - -0.3 2,381,048 + -1.4 2,381,093 + -2.3 2,381,145 + -0.1 2,381,317 + Dshi_2260 0.45 +0.0 2,381,381 + Dshi_2260 0.60 +0.3 2,381,389 - +1.3 2,381,399 - Dshi_2260 0.64 +1.9 2,381,399 - Dshi_2260 0.64 +1.0 2,381,576 + -0.3 2,381,596 + -0.5
Or see this region's nucleotide sequence