Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2197 and Dshi_2198 are separated by 79 nucleotides Dshi_2198 and Dshi_2199 are separated by 218 nucleotides Dshi_2199 and Dshi_2200 are separated by 77 nucleotides Dshi_2200 and Dshi_2201 are separated by 0 nucleotides
Dshi_2197: Dshi_2197 - ABC transporter related (RefSeq), at 2,324,302 to 2,325,957
_2197
Dshi_2198: Dshi_2198 - MOSC domain containing protein (RefSeq), at 2,326,037 to 2,326,516
_2198
Dshi_2199: Dshi_2199 - aspartate racemase (RefSeq), at 2,326,735 to 2,327,424
_2199
Dshi_2200: Dshi_2200 - regulatory protein TetR (RefSeq), at 2,327,502 to 2,328,092
_2200
Dshi_2201: Dshi_2201 - Putative protein-S-isoprenylcysteine methyltransferase-like protein (RefSeq), at 2,328,093 to 2,328,536
_2201
Position (kb)
2326
2327
2328 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2325.766 kb on - strand, within Dshi_2197 at 2325.766 kb on - strand, within Dshi_2197 at 2325.804 kb on + strand at 2325.869 kb on - strand at 2325.954 kb on + strand at 2325.969 kb on + strand at 2325.995 kb on + strand at 2326.003 kb on - strand at 2326.003 kb on - strand at 2326.018 kb on + strand at 2326.084 kb on + strand at 2326.142 kb on + strand, within Dshi_2198 at 2326.258 kb on + strand, within Dshi_2198 at 2326.271 kb on + strand, within Dshi_2198 at 2326.372 kb on - strand, within Dshi_2198 at 2326.607 kb on - strand at 2326.712 kb on + strand at 2326.785 kb on + strand at 2326.790 kb on + strand at 2326.798 kb on - strand at 2326.798 kb on - strand at 2326.827 kb on - strand, within Dshi_2199 at 2326.908 kb on - strand, within Dshi_2199 at 2326.921 kb on + strand, within Dshi_2199 at 2326.931 kb on + strand, within Dshi_2199 at 2327.017 kb on - strand, within Dshi_2199 at 2327.059 kb on + strand, within Dshi_2199 at 2327.216 kb on + strand, within Dshi_2199 at 2327.226 kb on + strand, within Dshi_2199 at 2327.235 kb on + strand, within Dshi_2199 at 2327.344 kb on + strand, within Dshi_2199 at 2327.385 kb on + strand at 2327.440 kb on - strand at 2327.503 kb on - strand at 2327.503 kb on - strand at 2327.503 kb on - strand at 2327.509 kb on + strand at 2327.517 kb on - strand at 2327.532 kb on + strand at 2327.593 kb on + strand, within Dshi_2200 at 2327.595 kb on + strand, within Dshi_2200 at 2327.603 kb on - strand, within Dshi_2200 at 2327.603 kb on - strand, within Dshi_2200 at 2327.603 kb on - strand, within Dshi_2200 at 2327.603 kb on - strand, within Dshi_2200 at 2327.713 kb on + strand, within Dshi_2200 at 2327.730 kb on + strand, within Dshi_2200 at 2327.769 kb on - strand, within Dshi_2200 at 2327.797 kb on + strand, within Dshi_2200 at 2327.797 kb on + strand, within Dshi_2200 at 2327.888 kb on - strand, within Dshi_2200 at 2327.906 kb on - strand, within Dshi_2200 at 2327.912 kb on + strand, within Dshi_2200 at 2327.929 kb on + strand, within Dshi_2200 at 2327.929 kb on + strand, within Dshi_2200 at 2327.932 kb on - strand, within Dshi_2200 at 2327.939 kb on + strand, within Dshi_2200 at 2328.055 kb on + strand at 2328.068 kb on - strand at 2328.076 kb on + strand at 2328.084 kb on - strand at 2328.085 kb on - strand at 2328.145 kb on - strand, within Dshi_2201 at 2328.221 kb on + strand, within Dshi_2201 at 2328.232 kb on + strand, within Dshi_2201 at 2328.316 kb on - strand, within Dshi_2201 at 2328.343 kb on - strand, within Dshi_2201 at 2328.387 kb on + strand, within Dshi_2201 at 2328.395 kb on + strand, within Dshi_2201 at 2328.395 kb on - strand, within Dshi_2201 at 2328.406 kb on + strand, within Dshi_2201
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 2,325,766 - Dshi_2197 0.88 -0.8 2,325,766 - Dshi_2197 0.88 -2.0 2,325,804 + -3.4 2,325,869 - -0.3 2,325,954 + -0.3 2,325,969 + +0.3 2,325,995 + +0.3 2,326,003 - +1.9 2,326,003 - -0.0 2,326,018 + +0.3 2,326,084 + -2.9 2,326,142 + Dshi_2198 0.22 -2.7 2,326,258 + Dshi_2198 0.46 +0.3 2,326,271 + Dshi_2198 0.49 +0.3 2,326,372 - Dshi_2198 0.70 -0.7 2,326,607 - +0.3 2,326,712 + -2.9 2,326,785 + -2.2 2,326,790 + -2.6 2,326,798 - +0.3 2,326,798 - -2.1 2,326,827 - Dshi_2199 0.13 -0.7 2,326,908 - Dshi_2199 0.25 -0.7 2,326,921 + Dshi_2199 0.27 +0.3 2,326,931 + Dshi_2199 0.28 +0.6 2,327,017 - Dshi_2199 0.41 -0.7 2,327,059 + Dshi_2199 0.47 -1.7 2,327,216 + Dshi_2199 0.70 -0.3 2,327,226 + Dshi_2199 0.71 -2.5 2,327,235 + Dshi_2199 0.72 +0.3 2,327,344 + Dshi_2199 0.88 -2.5 2,327,385 + -0.8 2,327,440 - +0.3 2,327,503 - -0.0 2,327,503 - -2.3 2,327,503 - -2.7 2,327,509 + -1.0 2,327,517 - +0.3 2,327,532 + -3.1 2,327,593 + Dshi_2200 0.15 -1.6 2,327,595 + Dshi_2200 0.16 +2.3 2,327,603 - Dshi_2200 0.17 -3.7 2,327,603 - Dshi_2200 0.17 +1.3 2,327,603 - Dshi_2200 0.17 -2.5 2,327,603 - Dshi_2200 0.17 -1.7 2,327,713 + Dshi_2200 0.36 -0.1 2,327,730 + Dshi_2200 0.39 -3.8 2,327,769 - Dshi_2200 0.45 +0.3 2,327,797 + Dshi_2200 0.50 +2.9 2,327,797 + Dshi_2200 0.50 -1.1 2,327,888 - Dshi_2200 0.65 -0.7 2,327,906 - Dshi_2200 0.68 -3.3 2,327,912 + Dshi_2200 0.69 -2.5 2,327,929 + Dshi_2200 0.72 -1.7 2,327,929 + Dshi_2200 0.72 +0.3 2,327,932 - Dshi_2200 0.73 -1.3 2,327,939 + Dshi_2200 0.74 -0.2 2,328,055 + -0.3 2,328,068 - -1.1 2,328,076 + +0.9 2,328,084 - -1.8 2,328,085 - +0.3 2,328,145 - Dshi_2201 0.12 -1.4 2,328,221 + Dshi_2201 0.29 -2.0 2,328,232 + Dshi_2201 0.31 -2.1 2,328,316 - Dshi_2201 0.50 +0.1 2,328,343 - Dshi_2201 0.56 +2.3 2,328,387 + Dshi_2201 0.66 -3.4 2,328,395 + Dshi_2201 0.68 -3.1 2,328,395 - Dshi_2201 0.68 -1.5 2,328,406 + Dshi_2201 0.70 -1.3
Or see this region's nucleotide sequence