Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_5006 and Dshi_2047 are separated by 0 nucleotides Dshi_2047 and Dshi_2048 are separated by 6 nucleotides Dshi_2048 and Dshi_2049 are separated by 21 nucleotides Dshi_2049 and Dshi_2050 overlap by 1 nucleotides
Dshi_5006: Dshi_5006 - hypothetical protein (NCBI), at 2,165,978 to 2,167,135
_5006
Dshi_2047: Dshi_2047 - hypothetical protein (RefSeq), at 2,167,136 to 2,167,762
_2047
Dshi_2048: Dshi_2048 - Mammalian cell entry related domain protein (RefSeq), at 2,167,769 to 2,169,508
_2048
Dshi_2049: Dshi_2049 - ABC transporter related (RefSeq), at 2,169,530 to 2,170,309
_2049
Dshi_2050: Dshi_2050 - protein of unknown function DUF140 (RefSeq), at 2,170,309 to 2,171,463
_2050
Position (kb)
2167
2168
2169
2170 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2166.794 kb on - strand, within Dshi_5006 at 2167.036 kb on - strand at 2167.037 kb on + strand at 2167.181 kb on - strand at 2167.355 kb on - strand, within Dshi_2047 at 2167.472 kb on + strand, within Dshi_2047 at 2167.552 kb on - strand, within Dshi_2047 at 2167.975 kb on - strand, within Dshi_2048 at 2168.095 kb on + strand, within Dshi_2048 at 2168.287 kb on + strand, within Dshi_2048 at 2168.495 kb on - strand, within Dshi_2048 at 2168.609 kb on - strand, within Dshi_2048 at 2168.640 kb on - strand, within Dshi_2048 at 2168.642 kb on - strand, within Dshi_2048 at 2168.690 kb on + strand, within Dshi_2048 at 2168.706 kb on + strand, within Dshi_2048 at 2168.755 kb on - strand, within Dshi_2048 at 2168.871 kb on + strand, within Dshi_2048 at 2168.879 kb on - strand, within Dshi_2048 at 2168.879 kb on - strand, within Dshi_2048 at 2168.970 kb on + strand, within Dshi_2048 at 2169.021 kb on + strand, within Dshi_2048 at 2169.080 kb on - strand, within Dshi_2048 at 2169.084 kb on + strand, within Dshi_2048 at 2169.086 kb on + strand, within Dshi_2048 at 2169.281 kb on + strand, within Dshi_2048 at 2169.286 kb on + strand, within Dshi_2048 at 2169.298 kb on + strand, within Dshi_2048 at 2169.306 kb on - strand, within Dshi_2048 at 2169.356 kb on + strand at 2169.370 kb on - strand at 2169.409 kb on - strand at 2169.411 kb on + strand at 2169.419 kb on - strand at 2169.419 kb on - strand at 2169.563 kb on - strand at 2169.596 kb on - strand at 2169.624 kb on - strand, within Dshi_2049 at 2169.687 kb on + strand, within Dshi_2049 at 2169.717 kb on - strand, within Dshi_2049 at 2169.749 kb on - strand, within Dshi_2049 at 2169.870 kb on - strand, within Dshi_2049 at 2169.958 kb on + strand, within Dshi_2049 at 2169.980 kb on + strand, within Dshi_2049 at 2169.980 kb on + strand, within Dshi_2049 at 2169.988 kb on - strand, within Dshi_2049 at 2170.014 kb on - strand, within Dshi_2049 at 2170.093 kb on + strand, within Dshi_2049 at 2170.204 kb on - strand, within Dshi_2049 at 2170.386 kb on - strand at 2170.433 kb on - strand, within Dshi_2050 at 2170.457 kb on + strand, within Dshi_2050 at 2170.490 kb on + strand, within Dshi_2050 at 2170.490 kb on + strand, within Dshi_2050
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 2,166,794 - Dshi_5006 0.70 -4.0 2,167,036 - -3.2 2,167,037 + -0.5 2,167,181 - -3.1 2,167,355 - Dshi_2047 0.35 -1.4 2,167,472 + Dshi_2047 0.54 -0.2 2,167,552 - Dshi_2047 0.66 +0.2 2,167,975 - Dshi_2048 0.12 -0.7 2,168,095 + Dshi_2048 0.19 -3.6 2,168,287 + Dshi_2048 0.30 +0.5 2,168,495 - Dshi_2048 0.42 -0.5 2,168,609 - Dshi_2048 0.48 +0.5 2,168,640 - Dshi_2048 0.50 +2.1 2,168,642 - Dshi_2048 0.50 +0.5 2,168,690 + Dshi_2048 0.53 -1.1 2,168,706 + Dshi_2048 0.54 -0.4 2,168,755 - Dshi_2048 0.57 +0.5 2,168,871 + Dshi_2048 0.63 -0.1 2,168,879 - Dshi_2048 0.64 +0.5 2,168,879 - Dshi_2048 0.64 -1.3 2,168,970 + Dshi_2048 0.69 -3.0 2,169,021 + Dshi_2048 0.72 +0.5 2,169,080 - Dshi_2048 0.75 -0.5 2,169,084 + Dshi_2048 0.76 -1.2 2,169,086 + Dshi_2048 0.76 -0.7 2,169,281 + Dshi_2048 0.87 -1.9 2,169,286 + Dshi_2048 0.87 +0.3 2,169,298 + Dshi_2048 0.88 -1.5 2,169,306 - Dshi_2048 0.88 -1.1 2,169,356 + -3.4 2,169,370 - -1.8 2,169,409 - -0.8 2,169,411 + +0.2 2,169,419 - -0.4 2,169,419 - +1.1 2,169,563 - +0.5 2,169,596 - +0.5 2,169,624 - Dshi_2049 0.12 -0.9 2,169,687 + Dshi_2049 0.20 -0.1 2,169,717 - Dshi_2049 0.24 +0.5 2,169,749 - Dshi_2049 0.28 -1.3 2,169,870 - Dshi_2049 0.44 +0.5 2,169,958 + Dshi_2049 0.55 +1.8 2,169,980 + Dshi_2049 0.58 -1.5 2,169,980 + Dshi_2049 0.58 +0.5 2,169,988 - Dshi_2049 0.59 -1.0 2,170,014 - Dshi_2049 0.62 -1.3 2,170,093 + Dshi_2049 0.72 -0.4 2,170,204 - Dshi_2049 0.86 +1.7 2,170,386 - -4.3 2,170,433 - Dshi_2050 0.11 +2.1 2,170,457 + Dshi_2050 0.13 +0.4 2,170,490 + Dshi_2050 0.16 -1.4 2,170,490 + Dshi_2050 0.16 +0.5
Or see this region's nucleotide sequence