Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1217 and Dshi_1218 are separated by 96 nucleotides Dshi_1218 and Dshi_1219 are separated by 12 nucleotides Dshi_1219 and Dshi_1220 are separated by 83 nucleotides
Dshi_1217: Dshi_1217 - AMP-dependent synthetase and ligase (RefSeq), at 1,256,903 to 1,258,444
_1217
Dshi_1218: Dshi_1218 - protein of unknown function DUF112 transmembrane (RefSeq), at 1,258,541 to 1,260,055
_1218
Dshi_1219: Dshi_1219 - hypothetical protein (RefSeq), at 1,260,068 to 1,260,553
_1219
Dshi_1220: Dshi_1220 - hypothetical protein (RefSeq), at 1,260,637 to 1,261,623
_1220
Position (kb)
1258
1259
1260
1261 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1257.665 kb on + strand, within Dshi_1217 at 1257.665 kb on + strand, within Dshi_1217 at 1257.854 kb on + strand, within Dshi_1217 at 1257.938 kb on - strand, within Dshi_1217 at 1258.033 kb on + strand, within Dshi_1217 at 1258.094 kb on + strand, within Dshi_1217 at 1258.423 kb on + strand at 1258.456 kb on - strand at 1258.507 kb on - strand at 1258.621 kb on + strand at 1258.631 kb on + strand at 1258.667 kb on + strand at 1258.680 kb on + strand at 1258.784 kb on + strand, within Dshi_1218 at 1258.866 kb on + strand, within Dshi_1218 at 1258.884 kb on - strand, within Dshi_1218 at 1258.943 kb on + strand, within Dshi_1218 at 1258.958 kb on - strand, within Dshi_1218 at 1258.958 kb on - strand, within Dshi_1218 at 1259.211 kb on + strand, within Dshi_1218 at 1259.229 kb on - strand, within Dshi_1218 at 1259.275 kb on + strand, within Dshi_1218 at 1259.418 kb on - strand, within Dshi_1218 at 1259.490 kb on + strand, within Dshi_1218 at 1259.498 kb on - strand, within Dshi_1218 at 1259.625 kb on + strand, within Dshi_1218 at 1259.834 kb on + strand, within Dshi_1218 at 1259.900 kb on + strand, within Dshi_1218 at 1259.925 kb on + strand at 1259.981 kb on + strand at 1260.010 kb on + strand at 1260.026 kb on + strand at 1260.050 kb on + strand at 1260.122 kb on - strand, within Dshi_1219 at 1260.196 kb on + strand, within Dshi_1219 at 1260.264 kb on - strand, within Dshi_1219 at 1260.298 kb on + strand, within Dshi_1219 at 1260.371 kb on - strand, within Dshi_1219 at 1260.482 kb on - strand, within Dshi_1219 at 1260.528 kb on - strand at 1260.554 kb on + strand at 1260.690 kb on + strand at 1260.730 kb on - strand at 1260.747 kb on + strand, within Dshi_1220
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 1,257,665 + Dshi_1217 0.49 -0.4 1,257,665 + Dshi_1217 0.49 -1.1 1,257,854 + Dshi_1217 0.62 +0.6 1,257,938 - Dshi_1217 0.67 -2.1 1,258,033 + Dshi_1217 0.73 -0.2 1,258,094 + Dshi_1217 0.77 -0.7 1,258,423 + -0.9 1,258,456 - -0.8 1,258,507 - -1.9 1,258,621 + +1.6 1,258,631 + -0.4 1,258,667 + +1.2 1,258,680 + +1.6 1,258,784 + Dshi_1218 0.16 -0.9 1,258,866 + Dshi_1218 0.21 +3.2 1,258,884 - Dshi_1218 0.23 +1.6 1,258,943 + Dshi_1218 0.27 -0.9 1,258,958 - Dshi_1218 0.28 -2.2 1,258,958 - Dshi_1218 0.28 +0.8 1,259,211 + Dshi_1218 0.44 -0.2 1,259,229 - Dshi_1218 0.45 -0.4 1,259,275 + Dshi_1218 0.48 +0.6 1,259,418 - Dshi_1218 0.58 +0.4 1,259,490 + Dshi_1218 0.63 -0.7 1,259,498 - Dshi_1218 0.63 -0.4 1,259,625 + Dshi_1218 0.72 -1.2 1,259,834 + Dshi_1218 0.85 -1.4 1,259,900 + Dshi_1218 0.90 -1.4 1,259,925 + -0.6 1,259,981 + +0.6 1,260,010 + -1.4 1,260,026 + +0.9 1,260,050 + +0.6 1,260,122 - Dshi_1219 0.11 -0.4 1,260,196 + Dshi_1219 0.26 -2.8 1,260,264 - Dshi_1219 0.40 +1.6 1,260,298 + Dshi_1219 0.47 -2.2 1,260,371 - Dshi_1219 0.62 -1.0 1,260,482 - Dshi_1219 0.85 +2.3 1,260,528 - -1.9 1,260,554 + -1.9 1,260,690 + -2.0 1,260,730 - +0.3 1,260,747 + Dshi_1220 0.11 -0.9
Or see this region's nucleotide sequence