Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1207 and Dshi_1208 are separated by 181 nucleotides Dshi_1208 and Dshi_1209 are separated by 91 nucleotides Dshi_1209 and Dshi_1210 are separated by 208 nucleotides
Dshi_1207: Dshi_1207 - glutamyl-tRNA(Gln) amidotransferase, B subunit (RefSeq), at 1,245,310 to 1,246,827
_1207
Dshi_1208: Dshi_1208 - hypothetical protein (RefSeq), at 1,247,009 to 1,247,605
_1208
Dshi_1209: Dshi_1209 - putative integral membrane sensor protein (RefSeq), at 1,247,697 to 1,248,803
_1209
Dshi_1210: Dshi_1210 - molybdopterin dehydrogenase FAD-binding (RefSeq), at 1,249,012 to 1,249,866
_1210
Position (kb)
1247
1248
1249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1246.911 kb on + strand at 1246.930 kb on - strand at 1247.037 kb on + strand at 1247.142 kb on + strand, within Dshi_1208 at 1247.229 kb on + strand, within Dshi_1208 at 1247.230 kb on + strand, within Dshi_1208 at 1247.240 kb on + strand, within Dshi_1208 at 1247.355 kb on + strand, within Dshi_1208 at 1247.360 kb on + strand, within Dshi_1208 at 1247.363 kb on - strand, within Dshi_1208 at 1247.426 kb on + strand, within Dshi_1208 at 1247.657 kb on - strand at 1247.783 kb on - strand at 1247.981 kb on - strand, within Dshi_1209 at 1248.042 kb on - strand, within Dshi_1209 at 1248.147 kb on - strand, within Dshi_1209 at 1248.276 kb on + strand, within Dshi_1209 at 1248.382 kb on + strand, within Dshi_1209 at 1248.495 kb on - strand, within Dshi_1209 at 1248.541 kb on - strand, within Dshi_1209 at 1248.716 kb on + strand at 1248.788 kb on + strand at 1249.019 kb on - strand at 1249.433 kb on - strand, within Dshi_1210 at 1249.543 kb on + strand, within Dshi_1210 at 1249.660 kb on - strand, within Dshi_1210 at 1249.660 kb on - strand, within Dshi_1210 at 1249.707 kb on - strand, within Dshi_1210
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 1,246,911 + +0.6 1,246,930 - +0.3 1,247,037 + -1.3 1,247,142 + Dshi_1208 0.22 +0.6 1,247,229 + Dshi_1208 0.37 -0.1 1,247,230 + Dshi_1208 0.37 +1.0 1,247,240 + Dshi_1208 0.39 -0.6 1,247,355 + Dshi_1208 0.58 +0.6 1,247,360 + Dshi_1208 0.59 -1.8 1,247,363 - Dshi_1208 0.59 -1.6 1,247,426 + Dshi_1208 0.70 -3.5 1,247,657 - -0.6 1,247,783 - +0.6 1,247,981 - Dshi_1209 0.26 -0.4 1,248,042 - Dshi_1209 0.31 -0.9 1,248,147 - Dshi_1209 0.41 +0.6 1,248,276 + Dshi_1209 0.52 +2.4 1,248,382 + Dshi_1209 0.62 +0.2 1,248,495 - Dshi_1209 0.72 +0.1 1,248,541 - Dshi_1209 0.76 +3.1 1,248,716 + -0.9 1,248,788 + +2.1 1,249,019 - -0.0 1,249,433 - Dshi_1210 0.49 +0.2 1,249,543 + Dshi_1210 0.62 -3.5 1,249,660 - Dshi_1210 0.76 -0.7 1,249,660 - Dshi_1210 0.76 -0.2 1,249,707 - Dshi_1210 0.81 -1.4
Or see this region's nucleotide sequence