Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0817 and Dshi_0818 overlap by 4 nucleotides Dshi_0818 and Dshi_0819 are separated by 24 nucleotides Dshi_0819 and Dshi_0820 are separated by 220 nucleotides Dshi_0820 and Dshi_5001 are separated by 81 nucleotides Dshi_5001 and Dshi_0821 are separated by 13 nucleotides
Dshi_0817: Dshi_0817 - hypothetical protein (RefSeq), at 823,262 to 823,522
_0817
Dshi_0818: Dshi_0818 - hypothetical protein (RefSeq), at 823,519 to 823,725
_0818
Dshi_0819: Dshi_0819 - hypothetical protein (RefSeq), at 823,750 to 824,046
_0819
Dshi_0820: Dshi_0820 - alpha/beta hydrolase fold (RefSeq), at 824,267 to 825,034
_0820
Dshi_5001: Dshi_5001 - hypothtical protein (RefSeq), at 825,116 to 825,505
_5001
Dshi_0821: Dshi_0821 - Glycine hydroxymethyltransferase (RefSeq), at 825,519 to 826,814
_0821
Position (kb)
824
825
826 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 823.267 kb on - strand at 823.288 kb on + strand, within Dshi_0817 at 823.306 kb on + strand, within Dshi_0817 at 823.321 kb on + strand, within Dshi_0817 at 823.384 kb on - strand, within Dshi_0817 at 823.573 kb on - strand, within Dshi_0818 at 823.708 kb on + strand at 823.793 kb on - strand, within Dshi_0819 at 823.830 kb on - strand, within Dshi_0819 at 823.857 kb on - strand, within Dshi_0819 at 823.908 kb on + strand, within Dshi_0819 at 823.942 kb on + strand, within Dshi_0819 at 823.957 kb on + strand, within Dshi_0819 at 823.974 kb on - strand, within Dshi_0819 at 824.006 kb on + strand, within Dshi_0819 at 824.077 kb on + strand at 824.085 kb on - strand at 824.101 kb on + strand at 824.101 kb on + strand at 824.109 kb on - strand at 824.167 kb on + strand at 824.319 kb on + strand at 824.451 kb on - strand, within Dshi_0820 at 824.451 kb on - strand, within Dshi_0820 at 824.686 kb on + strand, within Dshi_0820 at 824.794 kb on - strand, within Dshi_0820 at 824.876 kb on - strand, within Dshi_0820 at 824.917 kb on + strand, within Dshi_0820 at 824.969 kb on - strand at 825.020 kb on - strand at 825.064 kb on - strand at 825.121 kb on - strand at 825.125 kb on - strand at 825.298 kb on - strand, within Dshi_5001 at 825.309 kb on + strand, within Dshi_5001 at 825.379 kb on + strand, within Dshi_5001 at 825.494 kb on + strand at 825.525 kb on - strand at 825.528 kb on + strand at 825.692 kb on + strand, within Dshi_0821 at 825.713 kb on + strand, within Dshi_0821 at 825.799 kb on - strand, within Dshi_0821 at 825.842 kb on + strand, within Dshi_0821 at 825.850 kb on - strand, within Dshi_0821
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 823,267 - -1.2 823,288 + Dshi_0817 0.10 +0.8 823,306 + Dshi_0817 0.17 -0.0 823,321 + Dshi_0817 0.23 +3.1 823,384 - Dshi_0817 0.47 +0.8 823,573 - Dshi_0818 0.26 +1.0 823,708 + -2.9 823,793 - Dshi_0819 0.14 -0.5 823,830 - Dshi_0819 0.27 +0.6 823,857 - Dshi_0819 0.36 -4.1 823,908 + Dshi_0819 0.53 +0.8 823,942 + Dshi_0819 0.65 -2.0 823,957 + Dshi_0819 0.70 -2.7 823,974 - Dshi_0819 0.75 +0.1 824,006 + Dshi_0819 0.86 +2.4 824,077 + +0.8 824,085 - +1.2 824,101 + -2.2 824,101 + -0.2 824,109 - +0.2 824,167 + -2.2 824,319 + -1.0 824,451 - Dshi_0820 0.24 +1.2 824,451 - Dshi_0820 0.24 +1.5 824,686 + Dshi_0820 0.55 -0.2 824,794 - Dshi_0820 0.69 -3.7 824,876 - Dshi_0820 0.79 -0.8 824,917 + Dshi_0820 0.85 +1.8 824,969 - -1.2 825,020 - -1.8 825,064 - -0.2 825,121 - +0.5 825,125 - +0.7 825,298 - Dshi_5001 0.47 +0.8 825,309 + Dshi_5001 0.49 -0.8 825,379 + Dshi_5001 0.67 -0.5 825,494 + -1.3 825,525 - -2.4 825,528 + +0.8 825,692 + Dshi_0821 0.13 -0.1 825,713 + Dshi_0821 0.15 -3.3 825,799 - Dshi_0821 0.22 +2.8 825,842 + Dshi_0821 0.25 +0.6 825,850 - Dshi_0821 0.26 -0.8
Or see this region's nucleotide sequence