Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0342 and Dshi_0343 are separated by 90 nucleotides Dshi_0343 and Dshi_0344 are separated by 19 nucleotides Dshi_0344 and Dshi_0345 overlap by 4 nucleotides Dshi_0345 and Dshi_0346 are separated by 6 nucleotides
Dshi_0342: Dshi_0342 - tRNA (guanine-N1)-methyltransferase (RefSeq), at 329,524 to 330,321
_0342
Dshi_0343: Dshi_0343 - aminoglycoside phosphotransferase (RefSeq), at 330,412 to 331,401
_0343
Dshi_0344: Dshi_0344 - 16S rRNA processing protein RimM (RefSeq), at 331,421 to 331,921
_0344
Dshi_0345: Dshi_0345 - hypothetical protein (RefSeq), at 331,918 to 332,064
_0345
Dshi_0346: Dshi_0346 - protein of unknown function DUF55 (RefSeq), at 332,071 to 332,535
_0346
Position (kb) 330
331
332 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 329.530 kb on - strand at 330.227 kb on + strand, within Dshi_0342 at 330.437 kb on + strand at 330.526 kb on - strand, within Dshi_0343 at 330.537 kb on + strand, within Dshi_0343 at 330.577 kb on + strand, within Dshi_0343 at 330.609 kb on + strand, within Dshi_0343 at 330.728 kb on - strand, within Dshi_0343 at 330.743 kb on - strand, within Dshi_0343 at 330.764 kb on + strand, within Dshi_0343 at 330.808 kb on + strand, within Dshi_0343 at 330.871 kb on + strand, within Dshi_0343 at 331.104 kb on + strand, within Dshi_0343 at 331.104 kb on + strand, within Dshi_0343 at 331.104 kb on + strand, within Dshi_0343 at 331.112 kb on - strand, within Dshi_0343 at 331.211 kb on - strand, within Dshi_0343 at 331.294 kb on + strand, within Dshi_0343 at 331.294 kb on + strand, within Dshi_0343 at 331.302 kb on - strand, within Dshi_0343 at 331.394 kb on - strand at 331.427 kb on - strand at 331.635 kb on - strand, within Dshi_0344 at 331.857 kb on + strand, within Dshi_0344 at 331.910 kb on - strand at 332.033 kb on - strand, within Dshi_0345 at 332.099 kb on - strand at 332.314 kb on + strand, within Dshi_0346
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 329,530 - +0.2 330,227 + Dshi_0342 0.88 +2.8 330,437 + -1.6 330,526 - Dshi_0343 0.12 -1.2 330,537 + Dshi_0343 0.13 +2.8 330,577 + Dshi_0343 0.17 +0.8 330,609 + Dshi_0343 0.20 +0.8 330,728 - Dshi_0343 0.32 -0.2 330,743 - Dshi_0343 0.33 -1.5 330,764 + Dshi_0343 0.36 -3.2 330,808 + Dshi_0343 0.40 +0.8 330,871 + Dshi_0343 0.46 -1.3 331,104 + Dshi_0343 0.70 -1.4 331,104 + Dshi_0343 0.70 -0.2 331,104 + Dshi_0343 0.70 -2.9 331,112 - Dshi_0343 0.71 -0.2 331,211 - Dshi_0343 0.81 -2.6 331,294 + Dshi_0343 0.89 +0.8 331,294 + Dshi_0343 0.89 +0.3 331,302 - Dshi_0343 0.90 -0.2 331,394 - -0.2 331,427 - +0.0 331,635 - Dshi_0344 0.43 +0.8 331,857 + Dshi_0344 0.87 +0.8 331,910 - -1.8 332,033 - Dshi_0345 0.78 +0.8 332,099 - +0.8 332,314 + Dshi_0346 0.52 +0.8
Or see this region's nucleotide sequence