Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_R0003 and Dshi_0331 are separated by 6 nucleotides Dshi_0331 and Dshi_0332 overlap by 4 nucleotides Dshi_0332 and Dshi_0333 are separated by 62 nucleotides Dshi_0333 and Dshi_R0004 are separated by 155 nucleotides Dshi_R0004 and Dshi_0334 are separated by 51 nucleotides
Dshi_R0003: Dshi_R0003 - tRNA-Thr (RefSeq), at 319,728 to 319,803
_R0003
Dshi_0331: Dshi_0331 - protein of unknown function DUF37 (RefSeq), at 319,810 to 320,031
_0331
Dshi_0332: Dshi_0332 - ribonuclease P protein component (RefSeq), at 320,028 to 320,417
_0332
Dshi_0333: Dshi_0333 - ribosomal protein L34 (RefSeq), at 320,480 to 320,614
_0333
Dshi_R0004: Dshi_R0004 - tRNA-Arg (RefSeq), at 320,770 to 320,846
_R0004
Dshi_0334: Dshi_0334 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 320,898 to 322,301
_0334
Position (kb) 320
321 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 319.780 kb on - strand, within Dshi_R0003 at 319.872 kb on + strand, within Dshi_0331 at 319.953 kb on + strand, within Dshi_0331 at 319.983 kb on + strand, within Dshi_0331 at 321.235 kb on + strand, within Dshi_0334 at 321.243 kb on - strand, within Dshi_0334 at 321.379 kb on - strand, within Dshi_0334 at 321.490 kb on - strand, within Dshi_0334 at 321.563 kb on + strand, within Dshi_0334 at 321.563 kb on + strand, within Dshi_0334 at 321.578 kb on + strand, within Dshi_0334 at 321.748 kb on + strand, within Dshi_0334
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 319,780 - Dshi_R0003 0.68 -1.2 319,872 + Dshi_0331 0.28 -1.0 319,953 + Dshi_0331 0.64 -2.0 319,983 + Dshi_0331 0.78 -0.2 321,235 + Dshi_0334 0.24 +1.5 321,243 - Dshi_0334 0.25 -0.8 321,379 - Dshi_0334 0.34 -1.7 321,490 - Dshi_0334 0.42 -0.6 321,563 + Dshi_0334 0.47 -4.4 321,563 + Dshi_0334 0.47 -0.4 321,578 + Dshi_0334 0.48 +0.8 321,748 + Dshi_0334 0.61 -0.8
Or see this region's nucleotide sequence