Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0329 and Dshi_0330 are separated by 47 nucleotides Dshi_0330 and Dshi_R0003 are separated by 171 nucleotides Dshi_R0003 and Dshi_0331 are separated by 6 nucleotides Dshi_0331 and Dshi_0332 overlap by 4 nucleotides Dshi_0332 and Dshi_0333 are separated by 62 nucleotides
Dshi_0329: Dshi_0329 - diguanylate cyclase/phosphodiesterase (RefSeq), at 317,056 to 318,645
_0329
Dshi_0330: Dshi_0330 - PP-loop domain protein (RefSeq), at 318,693 to 319,556
_0330
Dshi_R0003: Dshi_R0003 - tRNA-Thr (RefSeq), at 319,728 to 319,803
_R0003
Dshi_0331: Dshi_0331 - protein of unknown function DUF37 (RefSeq), at 319,810 to 320,031
_0331
Dshi_0332: Dshi_0332 - ribonuclease P protein component (RefSeq), at 320,028 to 320,417
_0332
Dshi_0333: Dshi_0333 - ribosomal protein L34 (RefSeq), at 320,480 to 320,614
_0333
Position (kb) 318
319
320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 317.745 kb on - strand, within Dshi_0329 at 317.746 kb on + strand, within Dshi_0329 at 317.749 kb on - strand, within Dshi_0329 at 317.771 kb on + strand, within Dshi_0329 at 317.779 kb on - strand, within Dshi_0329 at 317.841 kb on - strand, within Dshi_0329 at 317.848 kb on - strand, within Dshi_0329 at 317.853 kb on - strand, within Dshi_0329 at 317.870 kb on - strand, within Dshi_0329 at 317.985 kb on + strand, within Dshi_0329 at 318.006 kb on + strand, within Dshi_0329 at 318.141 kb on + strand, within Dshi_0329 at 318.263 kb on + strand, within Dshi_0329 at 318.314 kb on + strand, within Dshi_0329 at 318.323 kb on - strand, within Dshi_0329 at 318.352 kb on - strand, within Dshi_0329 at 318.491 kb on + strand at 318.524 kb on - strand at 318.607 kb on + strand at 318.615 kb on - strand at 318.617 kb on - strand at 318.648 kb on + strand at 318.672 kb on + strand at 318.724 kb on + strand at 318.734 kb on + strand at 318.812 kb on + strand, within Dshi_0330 at 318.812 kb on + strand, within Dshi_0330 at 318.943 kb on + strand, within Dshi_0330 at 319.101 kb on - strand, within Dshi_0330 at 319.204 kb on - strand, within Dshi_0330 at 319.234 kb on + strand, within Dshi_0330 at 319.330 kb on + strand, within Dshi_0330 at 319.330 kb on + strand, within Dshi_0330 at 319.361 kb on + strand at 319.562 kb on - strand at 319.661 kb on + strand at 319.674 kb on - strand at 319.780 kb on - strand, within Dshi_R0003 at 319.872 kb on + strand, within Dshi_0331 at 319.953 kb on + strand, within Dshi_0331 at 319.983 kb on + strand, within Dshi_0331
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 317,745 - Dshi_0329 0.43 -0.9 317,746 + Dshi_0329 0.43 -2.0 317,749 - Dshi_0329 0.44 +3.1 317,771 + Dshi_0329 0.45 +0.1 317,779 - Dshi_0329 0.45 +2.8 317,841 - Dshi_0329 0.49 -3.5 317,848 - Dshi_0329 0.50 +0.8 317,853 - Dshi_0329 0.50 -0.8 317,870 - Dshi_0329 0.51 -0.2 317,985 + Dshi_0329 0.58 -1.1 318,006 + Dshi_0329 0.60 +0.8 318,141 + Dshi_0329 0.68 -1.7 318,263 + Dshi_0329 0.76 -1.8 318,314 + Dshi_0329 0.79 +4.1 318,323 - Dshi_0329 0.80 -3.1 318,352 - Dshi_0329 0.82 +0.8 318,491 + -1.2 318,524 - -0.9 318,607 + +0.8 318,615 - -0.8 318,617 - +2.2 318,648 + -2.5 318,672 + -3.4 318,724 + +0.4 318,734 + -2.2 318,812 + Dshi_0330 0.14 +1.2 318,812 + Dshi_0330 0.14 -0.8 318,943 + Dshi_0330 0.29 -1.9 319,101 - Dshi_0330 0.47 -3.3 319,204 - Dshi_0330 0.59 -0.4 319,234 + Dshi_0330 0.63 -3.7 319,330 + Dshi_0330 0.74 +2.1 319,330 + Dshi_0330 0.74 -0.6 319,361 + -1.2 319,562 - -1.8 319,661 + -1.6 319,674 - -2.5 319,780 - Dshi_R0003 0.68 -1.2 319,872 + Dshi_0331 0.28 -1.0 319,953 + Dshi_0331 0.64 -2.0 319,983 + Dshi_0331 0.78 -0.2
Or see this region's nucleotide sequence