Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0081 and Dshi_0082 are separated by 75 nucleotides Dshi_0082 and Dshi_0083 are separated by 105 nucleotides Dshi_0083 and Dshi_0084 are separated by 37 nucleotides
Dshi_0081: Dshi_0081 - 3-isopropylmalate dehydrogenase (RefSeq), at 80,080 to 81,186
_0081
Dshi_0082: Dshi_0082 - hypothetical protein (RefSeq), at 81,262 to 82,386
_0082
Dshi_0083: Dshi_0083 - 3-isopropylmalate dehydratase, small subunit (RefSeq), at 82,492 to 83,097
_0083
Dshi_0084: Dshi_0084 - hypothetical protein (RefSeq), at 83,135 to 83,662
_0084
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 80.596 kb on + strand, within Dshi_0081 at 81.250 kb on - strand at 81.344 kb on + strand at 81.352 kb on - strand at 81.357 kb on - strand at 81.375 kb on + strand, within Dshi_0082 at 81.379 kb on - strand, within Dshi_0082 at 81.388 kb on - strand, within Dshi_0082 at 81.497 kb on + strand, within Dshi_0082 at 81.603 kb on + strand, within Dshi_0082 at 81.909 kb on - strand, within Dshi_0082 at 81.960 kb on - strand, within Dshi_0082 at 82.000 kb on + strand, within Dshi_0082 at 82.000 kb on - strand, within Dshi_0082 at 82.143 kb on - strand, within Dshi_0082 at 82.170 kb on - strand, within Dshi_0082 at 82.260 kb on + strand, within Dshi_0082 at 82.260 kb on + strand, within Dshi_0082 at 82.260 kb on + strand, within Dshi_0082 at 82.262 kb on - strand, within Dshi_0082 at 82.345 kb on + strand at 82.350 kb on + strand at 82.353 kb on - strand at 82.455 kb on + strand at 82.516 kb on + strand at 82.516 kb on + strand at 82.524 kb on - strand at 82.551 kb on + strand at 82.559 kb on - strand, within Dshi_0083 at 82.559 kb on - strand, within Dshi_0083 at 83.016 kb on + strand, within Dshi_0083 at 83.167 kb on - strand at 83.191 kb on - strand, within Dshi_0084 at 83.213 kb on + strand, within Dshi_0084 at 83.213 kb on - strand, within Dshi_0084 at 83.213 kb on - strand, within Dshi_0084 at 83.330 kb on - strand, within Dshi_0084 at 83.341 kb on + strand, within Dshi_0084
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 80,596 + Dshi_0081 0.47 +1.9 81,250 - -0.6 81,344 + +1.9 81,352 - -0.4 81,357 - -1.3 81,375 + Dshi_0082 0.10 +0.9 81,379 - Dshi_0082 0.10 -3.6 81,388 - Dshi_0082 0.11 -1.7 81,497 + Dshi_0082 0.21 -2.1 81,603 + Dshi_0082 0.30 -1.1 81,909 - Dshi_0082 0.58 -2.4 81,960 - Dshi_0082 0.62 -2.5 82,000 + Dshi_0082 0.66 +1.2 82,000 - Dshi_0082 0.66 -2.2 82,143 - Dshi_0082 0.78 -1.6 82,170 - Dshi_0082 0.81 +0.9 82,260 + Dshi_0082 0.89 -0.2 82,260 + Dshi_0082 0.89 -2.5 82,260 + Dshi_0082 0.89 +0.1 82,262 - Dshi_0082 0.89 +0.4 82,345 + +0.2 82,350 + +1.9 82,353 - -0.9 82,455 + -1.9 82,516 + -0.5 82,516 + -0.4 82,524 - -0.1 82,551 + +0.9 82,559 - Dshi_0083 0.11 +0.9 82,559 - Dshi_0083 0.11 +0.9 83,016 + Dshi_0083 0.86 -1.4 83,167 - -0.1 83,191 - Dshi_0084 0.11 +0.9 83,213 + Dshi_0084 0.15 +3.3 83,213 - Dshi_0084 0.15 -1.9 83,213 - Dshi_0084 0.15 -1.1 83,330 - Dshi_0084 0.37 +3.3 83,341 + Dshi_0084 0.39 -1.5
Or see this region's nucleotide sequence