Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_962 and HP15_963 are separated by 208 nucleotides HP15_963 and HP15_964 are separated by 83 nucleotides HP15_964 and HP15_965 are separated by 102 nucleotides HP15_965 and HP15_966 are separated by 66 nucleotides
HP15_962: HP15_962 - orf153EGC121-like protein, at 977,827 to 978,132
_962
HP15_963: HP15_963 - conserved hypothetical protein, at 978,341 to 978,550
_963
HP15_964: HP15_964 - conserved hypothetical protein, at 978,634 to 979,386
_964
HP15_965: HP15_965 - hypothetical protein, at 979,489 to 979,923
_965
HP15_966: HP15_966 - ABC transporter related protein, at 979,990 to 980,289
_966
Position (kb) 978
979
980 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 977.715 kb on + strand at 977.723 kb on - strand at 977.777 kb on + strand at 977.777 kb on + strand at 977.785 kb on - strand at 977.792 kb on - strand at 977.840 kb on - strand at 977.900 kb on - strand, within HP15_962 at 977.905 kb on - strand, within HP15_962 at 977.915 kb on - strand, within HP15_962 at 977.964 kb on + strand, within HP15_962 at 978.028 kb on + strand, within HP15_962 at 978.028 kb on + strand, within HP15_962 at 978.029 kb on - strand, within HP15_962 at 978.029 kb on - strand, within HP15_962 at 978.036 kb on - strand, within HP15_962 at 978.074 kb on + strand, within HP15_962 at 978.215 kb on + strand at 978.358 kb on + strand at 978.400 kb on + strand, within HP15_963 at 978.467 kb on + strand, within HP15_963 at 978.467 kb on + strand, within HP15_963 at 978.467 kb on + strand, within HP15_963 at 978.475 kb on - strand, within HP15_963 at 978.475 kb on - strand, within HP15_963 at 978.475 kb on - strand, within HP15_963 at 978.475 kb on - strand, within HP15_963 at 978.475 kb on - strand, within HP15_963 at 978.475 kb on - strand, within HP15_963 at 978.485 kb on - strand, within HP15_963 at 978.509 kb on - strand, within HP15_963 at 978.613 kb on + strand at 978.863 kb on + strand, within HP15_964 at 978.890 kb on + strand, within HP15_964 at 978.955 kb on + strand, within HP15_964 at 979.120 kb on + strand, within HP15_964 at 979.151 kb on - strand, within HP15_964 at 979.182 kb on + strand, within HP15_964 at 979.230 kb on - strand, within HP15_964 at 979.438 kb on - strand at 979.517 kb on - strand at 979.525 kb on + strand at 979.537 kb on + strand, within HP15_965 at 979.557 kb on + strand, within HP15_965 at 979.597 kb on + strand, within HP15_965 at 979.605 kb on - strand, within HP15_965 at 979.658 kb on - strand, within HP15_965 at 979.725 kb on - strand, within HP15_965 at 979.808 kb on + strand, within HP15_965 at 979.821 kb on - strand, within HP15_965 at 979.935 kb on - strand at 980.176 kb on - strand, within HP15_966 at 980.177 kb on + strand, within HP15_966 at 980.185 kb on - strand, within HP15_966 at 980.371 kb on + strand at 980.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 977,715 + -1.0 977,723 - -0.1 977,777 + -1.0 977,777 + -2.6 977,785 - -0.0 977,792 - -0.6 977,840 - +0.8 977,900 - HP15_962 0.24 +0.2 977,905 - HP15_962 0.25 -1.1 977,915 - HP15_962 0.29 +2.5 977,964 + HP15_962 0.45 +0.8 978,028 + HP15_962 0.66 -0.5 978,028 + HP15_962 0.66 +3.6 978,029 - HP15_962 0.66 -0.2 978,029 - HP15_962 0.66 +1.0 978,036 - HP15_962 0.68 -0.4 978,074 + HP15_962 0.81 -0.6 978,215 + -0.2 978,358 + -3.7 978,400 + HP15_963 0.28 +0.5 978,467 + HP15_963 0.60 +0.9 978,467 + HP15_963 0.60 -0.3 978,467 + HP15_963 0.60 -0.1 978,475 - HP15_963 0.64 -1.4 978,475 - HP15_963 0.64 -0.2 978,475 - HP15_963 0.64 +0.1 978,475 - HP15_963 0.64 +1.5 978,475 - HP15_963 0.64 -0.9 978,475 - HP15_963 0.64 +0.3 978,485 - HP15_963 0.69 -0.7 978,509 - HP15_963 0.80 -0.9 978,613 + +0.0 978,863 + HP15_964 0.30 +2.1 978,890 + HP15_964 0.34 -0.3 978,955 + HP15_964 0.43 -0.1 979,120 + HP15_964 0.65 +0.0 979,151 - HP15_964 0.69 -0.4 979,182 + HP15_964 0.73 -1.2 979,230 - HP15_964 0.79 +0.2 979,438 - +2.4 979,517 - -1.4 979,525 + +0.0 979,537 + HP15_965 0.11 +2.3 979,557 + HP15_965 0.16 +0.1 979,597 + HP15_965 0.25 +1.0 979,605 - HP15_965 0.27 -0.7 979,658 - HP15_965 0.39 +0.5 979,725 - HP15_965 0.54 -2.7 979,808 + HP15_965 0.73 +1.9 979,821 - HP15_965 0.76 +1.0 979,935 - +2.0 980,176 - HP15_966 0.62 -0.4 980,177 + HP15_966 0.62 -0.4 980,185 - HP15_966 0.65 -0.9 980,371 + -0.4 980,371 + -0.4
Or see this region's nucleotide sequence