Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_886 and HP15_887 are separated by 23 nucleotides HP15_887 and HP15_888 are separated by 6 nucleotides HP15_888 and HP15_889 are separated by 47 nucleotides HP15_889 and HP15_890 are separated by 19 nucleotides HP15_890 and HP15_891 overlap by 4 nucleotides
HP15_886: HP15_886 - pseudouridine synthase family 1 protein, at 901,827 to 902,765
_886
HP15_887: HP15_887 - hypothetical protein, at 902,789 to 902,893
_887
HP15_888: HP15_888 - conserved hypothetical protein, membrane, at 902,900 to 903,295
_888
HP15_889: HP15_889 - cobalamin synthesis protein/P47K family protein, at 903,343 to 904,329
_889
HP15_890: HP15_890 - methyltransferase-related protein, at 904,349 to 904,972
_890
HP15_891: HP15_891 - conserved hypothetical protein, at 904,969 to 905,970
_891
Position (kb) 903
904
905 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 902.360 kb on - strand, within HP15_886 at 902.490 kb on + strand, within HP15_886 at 902.542 kb on + strand, within HP15_886 at 902.555 kb on - strand, within HP15_886 at 902.606 kb on + strand, within HP15_886 at 902.942 kb on + strand, within HP15_888 at 903.265 kb on + strand at 903.451 kb on + strand, within HP15_889 at 903.488 kb on + strand, within HP15_889 at 903.669 kb on - strand, within HP15_889 at 903.841 kb on - strand, within HP15_889 at 903.967 kb on + strand, within HP15_889 at 904.064 kb on + strand, within HP15_889 at 904.146 kb on + strand, within HP15_889 at 904.154 kb on - strand, within HP15_889 at 904.204 kb on + strand, within HP15_889 at 904.207 kb on - strand, within HP15_889 at 904.242 kb on - strand at 904.468 kb on - strand, within HP15_890 at 904.610 kb on - strand, within HP15_890 at 904.610 kb on - strand, within HP15_890 at 904.828 kb on - strand, within HP15_890 at 904.856 kb on + strand, within HP15_890 at 904.856 kb on + strand, within HP15_890 at 904.864 kb on - strand, within HP15_890 at 904.942 kb on + strand at 904.998 kb on + strand at 905.018 kb on - strand at 905.234 kb on + strand, within HP15_891 at 905.288 kb on + strand, within HP15_891 at 905.290 kb on + strand, within HP15_891
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 902,360 - HP15_886 0.57 -3.1 902,490 + HP15_886 0.71 -0.5 902,542 + HP15_886 0.76 +0.4 902,555 - HP15_886 0.78 -0.3 902,606 + HP15_886 0.83 -0.2 902,942 + HP15_888 0.11 +1.9 903,265 + -0.5 903,451 + HP15_889 0.11 -1.1 903,488 + HP15_889 0.15 +1.6 903,669 - HP15_889 0.33 -1.8 903,841 - HP15_889 0.50 -0.1 903,967 + HP15_889 0.63 -1.0 904,064 + HP15_889 0.73 +0.7 904,146 + HP15_889 0.81 -0.9 904,154 - HP15_889 0.82 -0.4 904,204 + HP15_889 0.87 -1.2 904,207 - HP15_889 0.88 -1.0 904,242 - +0.3 904,468 - HP15_890 0.19 +0.3 904,610 - HP15_890 0.42 -0.8 904,610 - HP15_890 0.42 +1.4 904,828 - HP15_890 0.77 +1.5 904,856 + HP15_890 0.81 -0.4 904,856 + HP15_890 0.81 +0.7 904,864 - HP15_890 0.83 +0.9 904,942 + +2.2 904,998 + -0.7 905,018 - -1.7 905,234 + HP15_891 0.26 +1.9 905,288 + HP15_891 0.32 +0.4 905,290 + HP15_891 0.32 -1.5
Or see this region's nucleotide sequence