Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_843 and HP15_844 are separated by 3 nucleotides HP15_844 and HP15_845 are separated by 226 nucleotides HP15_845 and HP15_846 overlap by 4 nucleotides HP15_846 and HP15_847 are separated by 40 nucleotides HP15_847 and HP15_848 are separated by 112 nucleotides
HP15_843: HP15_843 - PhnA protein, at 862,138 to 862,476
_843
HP15_844: HP15_844 - protein containing DUF533, at 862,480 to 863,199
_844
HP15_845: HP15_845 - sensory box/GGDEF family protein, at 863,426 to 865,702
_845
HP15_846: HP15_846 - DR1885-like metal-binding protein, at 865,699 to 866,307
_846
HP15_847: HP15_847 - hypothetical protein, at 866,348 to 866,476
_847
HP15_848: HP15_848 - protein containing DUF980, at 866,589 to 867,419
_848
Position (kb) 863
864
865
866 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 862.522 kb on + strand at 862.573 kb on - strand, within HP15_844 at 862.633 kb on + strand, within HP15_844 at 862.644 kb on + strand, within HP15_844 at 862.665 kb on - strand, within HP15_844 at 862.670 kb on - strand, within HP15_844 at 862.670 kb on - strand, within HP15_844 at 862.682 kb on - strand, within HP15_844 at 862.796 kb on + strand, within HP15_844 at 862.841 kb on + strand, within HP15_844 at 862.841 kb on - strand, within HP15_844 at 863.000 kb on - strand, within HP15_844 at 863.092 kb on - strand, within HP15_844 at 863.106 kb on - strand, within HP15_844 at 863.132 kb on - strand at 863.146 kb on - strand at 863.153 kb on + strand at 863.161 kb on - strand at 863.184 kb on - strand at 863.372 kb on + strand at 863.380 kb on - strand at 863.463 kb on + strand at 863.499 kb on - strand at 863.504 kb on - strand at 863.564 kb on - strand at 863.578 kb on + strand at 863.627 kb on - strand at 863.695 kb on + strand, within HP15_845 at 863.794 kb on + strand, within HP15_845 at 863.896 kb on + strand, within HP15_845 at 863.903 kb on - strand, within HP15_845 at 863.904 kb on - strand, within HP15_845 at 864.000 kb on - strand, within HP15_845 at 864.002 kb on + strand, within HP15_845 at 864.024 kb on + strand, within HP15_845 at 864.092 kb on - strand, within HP15_845 at 864.138 kb on + strand, within HP15_845 at 864.168 kb on + strand, within HP15_845 at 864.168 kb on + strand, within HP15_845 at 864.245 kb on + strand, within HP15_845 at 864.256 kb on - strand, within HP15_845 at 864.324 kb on + strand, within HP15_845 at 864.345 kb on - strand, within HP15_845 at 864.503 kb on - strand, within HP15_845 at 864.636 kb on - strand, within HP15_845 at 864.637 kb on - strand, within HP15_845 at 864.750 kb on + strand, within HP15_845 at 864.769 kb on - strand, within HP15_845 at 864.852 kb on + strand, within HP15_845 at 864.860 kb on - strand, within HP15_845 at 864.990 kb on - strand, within HP15_845 at 865.332 kb on - strand, within HP15_845 at 865.419 kb on - strand, within HP15_845 at 865.524 kb on + strand at 865.586 kb on + strand at 865.591 kb on + strand at 865.630 kb on + strand at 865.655 kb on + strand at 865.655 kb on + strand at 865.655 kb on + strand at 865.704 kb on - strand at 865.833 kb on + strand, within HP15_846 at 866.072 kb on - strand, within HP15_846 at 866.164 kb on + strand, within HP15_846 at 866.189 kb on + strand, within HP15_846 at 866.372 kb on + strand, within HP15_847 at 866.380 kb on - strand, within HP15_847 at 866.445 kb on + strand, within HP15_847 at 866.558 kb on - strand at 866.633 kb on - strand at 866.641 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 862,522 + -0.8 862,573 - HP15_844 0.13 -0.2 862,633 + HP15_844 0.21 -0.7 862,644 + HP15_844 0.23 -0.7 862,665 - HP15_844 0.26 -0.0 862,670 - HP15_844 0.26 +0.1 862,670 - HP15_844 0.26 +1.7 862,682 - HP15_844 0.28 +0.6 862,796 + HP15_844 0.44 +0.6 862,841 + HP15_844 0.50 +1.6 862,841 - HP15_844 0.50 +0.3 863,000 - HP15_844 0.72 -0.9 863,092 - HP15_844 0.85 -1.4 863,106 - HP15_844 0.87 -1.0 863,132 - +0.2 863,146 - +1.9 863,153 + +0.0 863,161 - -1.1 863,184 - +2.6 863,372 + +1.8 863,380 - +0.7 863,463 + +1.9 863,499 - +1.9 863,504 - +2.0 863,564 - +1.0 863,578 + -0.3 863,627 - -0.2 863,695 + HP15_845 0.12 +1.2 863,794 + HP15_845 0.16 -0.6 863,896 + HP15_845 0.21 -1.8 863,903 - HP15_845 0.21 +1.5 863,904 - HP15_845 0.21 -0.1 864,000 - HP15_845 0.25 +0.3 864,002 + HP15_845 0.25 -1.4 864,024 + HP15_845 0.26 -0.1 864,092 - HP15_845 0.29 -0.4 864,138 + HP15_845 0.31 +0.4 864,168 + HP15_845 0.33 +1.9 864,168 + HP15_845 0.33 +1.5 864,245 + HP15_845 0.36 +1.6 864,256 - HP15_845 0.36 -0.0 864,324 + HP15_845 0.39 -0.7 864,345 - HP15_845 0.40 -1.3 864,503 - HP15_845 0.47 +3.1 864,636 - HP15_845 0.53 -0.5 864,637 - HP15_845 0.53 -0.0 864,750 + HP15_845 0.58 +1.5 864,769 - HP15_845 0.59 +1.8 864,852 + HP15_845 0.63 +1.2 864,860 - HP15_845 0.63 +2.1 864,990 - HP15_845 0.69 +1.7 865,332 - HP15_845 0.84 +0.1 865,419 - HP15_845 0.88 +0.2 865,524 + -1.0 865,586 + -0.9 865,591 + -1.3 865,630 + +1.3 865,655 + +0.5 865,655 + -0.2 865,655 + -0.0 865,704 - -1.1 865,833 + HP15_846 0.22 +0.4 866,072 - HP15_846 0.61 +1.5 866,164 + HP15_846 0.76 -0.6 866,189 + HP15_846 0.80 +4.8 866,372 + HP15_847 0.19 -0.4 866,380 - HP15_847 0.25 +2.6 866,445 + HP15_847 0.75 +1.8 866,558 - -0.9 866,633 - +1.1 866,641 + +1.1
Or see this region's nucleotide sequence