Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_739 and HP15_740 are separated by 114 nucleotides HP15_740 and HP15_741 are separated by 226 nucleotides HP15_741 and HP15_742 are separated by 127 nucleotides
HP15_739: HP15_739 - glycosyl transferase, group 1 family protein, at 746,970 to 748,190
_739
HP15_740: HP15_740 - oxidoreductase Tas, aldo/keto reductase family protein, at 748,305 to 749,342
_740
HP15_741: HP15_741 - DNA polymerase IV-like protein, at 749,569 to 749,892
_741
HP15_742: HP15_742 - acetyl-CoA hydrolase, at 750,020 to 752,230
_742
Position (kb) 748
749
750 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 747.558 kb on - strand, within HP15_739 at 747.595 kb on - strand, within HP15_739 at 747.611 kb on - strand, within HP15_739 at 747.913 kb on + strand, within HP15_739 at 747.931 kb on - strand, within HP15_739 at 748.009 kb on - strand, within HP15_739 at 748.029 kb on + strand, within HP15_739 at 748.051 kb on - strand, within HP15_739 at 748.051 kb on - strand, within HP15_739 at 748.099 kb on - strand at 748.201 kb on - strand at 748.214 kb on - strand at 748.339 kb on + strand at 748.385 kb on - strand at 748.385 kb on - strand at 748.399 kb on + strand at 748.420 kb on - strand, within HP15_740 at 748.439 kb on - strand, within HP15_740 at 748.465 kb on - strand, within HP15_740 at 748.465 kb on - strand, within HP15_740 at 748.465 kb on - strand, within HP15_740 at 748.486 kb on + strand, within HP15_740 at 748.497 kb on + strand, within HP15_740 at 748.661 kb on + strand, within HP15_740 at 748.666 kb on + strand, within HP15_740 at 748.699 kb on + strand, within HP15_740 at 748.980 kb on - strand, within HP15_740 at 749.073 kb on - strand, within HP15_740 at 749.121 kb on + strand, within HP15_740 at 749.201 kb on - strand, within HP15_740 at 749.219 kb on + strand, within HP15_740 at 749.281 kb on + strand at 749.289 kb on - strand at 749.373 kb on + strand at 749.405 kb on - strand at 749.480 kb on + strand at 749.590 kb on + strand at 749.714 kb on - strand, within HP15_741 at 749.740 kb on - strand, within HP15_741 at 749.771 kb on + strand, within HP15_741 at 749.779 kb on - strand, within HP15_741 at 749.779 kb on - strand, within HP15_741 at 749.853 kb on + strand, within HP15_741 at 749.886 kb on + strand at 749.894 kb on - strand at 749.894 kb on - strand at 750.293 kb on + strand, within HP15_742
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 747,558 - HP15_739 0.48 +0.5 747,595 - HP15_739 0.51 +0.6 747,611 - HP15_739 0.52 +1.2 747,913 + HP15_739 0.77 +0.9 747,931 - HP15_739 0.79 -0.6 748,009 - HP15_739 0.85 +0.5 748,029 + HP15_739 0.87 +2.1 748,051 - HP15_739 0.89 -0.4 748,051 - HP15_739 0.89 +0.1 748,099 - +1.1 748,201 - -0.5 748,214 - -0.8 748,339 + -0.6 748,385 - -0.0 748,385 - +1.4 748,399 + +1.5 748,420 - HP15_740 0.11 -1.0 748,439 - HP15_740 0.13 +1.9 748,465 - HP15_740 0.15 -1.4 748,465 - HP15_740 0.15 -0.8 748,465 - HP15_740 0.15 -0.1 748,486 + HP15_740 0.17 -1.1 748,497 + HP15_740 0.18 -0.3 748,661 + HP15_740 0.34 -0.2 748,666 + HP15_740 0.35 +1.7 748,699 + HP15_740 0.38 -0.5 748,980 - HP15_740 0.65 -2.6 749,073 - HP15_740 0.74 -0.5 749,121 + HP15_740 0.79 +1.5 749,201 - HP15_740 0.86 -0.1 749,219 + HP15_740 0.88 +1.5 749,281 + -3.2 749,289 - -0.1 749,373 + +1.4 749,405 - -1.0 749,480 + +0.3 749,590 + +2.2 749,714 - HP15_741 0.45 -0.8 749,740 - HP15_741 0.53 -0.5 749,771 + HP15_741 0.62 -0.5 749,779 - HP15_741 0.65 -0.2 749,779 - HP15_741 0.65 -0.5 749,853 + HP15_741 0.88 -0.9 749,886 + -0.2 749,894 - -0.1 749,894 - -1.0 750,293 + HP15_742 0.12 +1.8
Or see this region's nucleotide sequence