Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_615 and HP15_616 are separated by 155 nucleotides HP15_616 and HP15_617 are separated by 46 nucleotides HP15_617 and HP15_618 are separated by 80 nucleotides
HP15_615: HP15_615 - 6-phosphofructokinase, at 636,969 to 638,231
_615
HP15_616: HP15_616 - UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase, at 638,387 to 639,781
_616
HP15_617: HP15_617 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 639,828 to 640,475
_617
HP15_618: HP15_618 - acyl-CoA dehydrogenase domain protein, at 640,556 to 641,737
_618
Position (kb) 638
639
640 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 638.305 kb on + strand at 638.313 kb on - strand at 638.465 kb on - strand at 638.472 kb on + strand at 638.517 kb on - strand at 638.521 kb on + strand at 638.602 kb on - strand, within HP15_616 at 638.692 kb on + strand, within HP15_616 at 638.700 kb on - strand, within HP15_616 at 638.728 kb on + strand, within HP15_616 at 638.772 kb on + strand, within HP15_616 at 638.919 kb on + strand, within HP15_616 at 638.982 kb on - strand, within HP15_616 at 639.098 kb on + strand, within HP15_616 at 639.098 kb on + strand, within HP15_616 at 639.224 kb on + strand, within HP15_616 at 639.232 kb on - strand, within HP15_616 at 639.278 kb on + strand, within HP15_616 at 639.284 kb on - strand, within HP15_616 at 639.302 kb on - strand, within HP15_616 at 639.425 kb on + strand, within HP15_616 at 639.450 kb on + strand, within HP15_616 at 639.463 kb on - strand, within HP15_616 at 639.500 kb on - strand, within HP15_616 at 639.500 kb on - strand, within HP15_616 at 639.500 kb on - strand, within HP15_616 at 639.504 kb on + strand, within HP15_616 at 639.693 kb on + strand at 639.756 kb on - strand at 640.469 kb on + strand at 640.470 kb on + strand at 640.488 kb on - strand at 640.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 638,305 + -0.3 638,313 - +1.7 638,465 - -1.1 638,472 + +1.3 638,517 - -0.1 638,521 + -1.1 638,602 - HP15_616 0.15 +1.1 638,692 + HP15_616 0.22 -2.9 638,700 - HP15_616 0.22 +2.1 638,728 + HP15_616 0.24 +1.2 638,772 + HP15_616 0.28 +1.2 638,919 + HP15_616 0.38 -0.2 638,982 - HP15_616 0.43 +1.3 639,098 + HP15_616 0.51 +1.7 639,098 + HP15_616 0.51 -0.2 639,224 + HP15_616 0.60 -2.5 639,232 - HP15_616 0.61 +2.4 639,278 + HP15_616 0.64 +0.1 639,284 - HP15_616 0.64 -1.0 639,302 - HP15_616 0.66 +0.6 639,425 + HP15_616 0.74 +1.5 639,450 + HP15_616 0.76 +0.3 639,463 - HP15_616 0.77 -0.1 639,500 - HP15_616 0.80 -1.7 639,500 - HP15_616 0.80 -1.5 639,500 - HP15_616 0.80 -0.3 639,504 + HP15_616 0.80 -0.5 639,693 + -0.3 639,756 - +1.0 640,469 + -0.6 640,470 + -1.2 640,488 - +1.6 640,493 + +0.8
Or see this region's nucleotide sequence