Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_p187g83 and HP15_p187g84 are separated by 19 nucleotides HP15_p187g84 and HP15_p187g85 are separated by 302 nucleotides HP15_p187g85 and HP15_p187g86 are separated by 180 nucleotides HP15_p187g86 and HP15_p187g87 are separated by 6 nucleotides
HP15_p187g83: HP15_p187g83 - conserved hypothetical protein, membrane, at 80,577 to 81,798
_p187g83
HP15_p187g84: HP15_p187g84 - hypothetical protein, at 81,818 to 82,462
_p187g84
HP15_p187g85: HP15_p187g85 - conserved hypothetical protein, membrane, at 82,765 to 83,841
_p187g85
HP15_p187g86: HP15_p187g86 - hypothetical protein, at 84,022 to 84,147
_p187g86
HP15_p187g87: HP15_p187g87 - signal transduction histidine kinase, at 84,154 to 85,413
_p187g87
Position (kb) 82
83
84 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 81.767 kb on - strand at 81.779 kb on - strand at 81.793 kb on + strand at 81.801 kb on + strand at 81.801 kb on - strand at 81.801 kb on - strand at 81.801 kb on - strand at 81.801 kb on - strand at 81.841 kb on + strand at 81.849 kb on - strand at 81.859 kb on - strand at 81.882 kb on + strand at 81.892 kb on - strand, within HP15_p187g84 at 81.941 kb on - strand, within HP15_p187g84 at 81.951 kb on + strand, within HP15_p187g84 at 81.951 kb on - strand, within HP15_p187g84 at 81.953 kb on - strand, within HP15_p187g84 at 81.958 kb on + strand, within HP15_p187g84 at 81.958 kb on + strand, within HP15_p187g84 at 81.958 kb on + strand, within HP15_p187g84 at 81.966 kb on - strand, within HP15_p187g84 at 81.966 kb on - strand, within HP15_p187g84 at 81.966 kb on - strand, within HP15_p187g84 at 81.970 kb on + strand, within HP15_p187g84 at 81.970 kb on + strand, within HP15_p187g84 at 81.978 kb on - strand, within HP15_p187g84 at 81.991 kb on + strand, within HP15_p187g84 at 81.992 kb on + strand, within HP15_p187g84 at 82.013 kb on + strand, within HP15_p187g84 at 82.013 kb on + strand, within HP15_p187g84 at 82.024 kb on + strand, within HP15_p187g84 at 82.024 kb on + strand, within HP15_p187g84 at 82.024 kb on + strand, within HP15_p187g84 at 82.024 kb on + strand, within HP15_p187g84 at 82.024 kb on + strand, within HP15_p187g84 at 82.031 kb on - strand, within HP15_p187g84 at 82.032 kb on - strand, within HP15_p187g84 at 82.032 kb on - strand, within HP15_p187g84 at 82.064 kb on + strand, within HP15_p187g84 at 82.115 kb on + strand, within HP15_p187g84 at 82.115 kb on + strand, within HP15_p187g84 at 82.116 kb on - strand, within HP15_p187g84 at 82.120 kb on + strand, within HP15_p187g84 at 82.120 kb on + strand, within HP15_p187g84 at 82.123 kb on - strand, within HP15_p187g84 at 82.128 kb on - strand, within HP15_p187g84 at 82.142 kb on - strand, within HP15_p187g84 at 82.153 kb on + strand, within HP15_p187g84 at 82.201 kb on - strand, within HP15_p187g84 at 82.217 kb on - strand, within HP15_p187g84 at 82.262 kb on + strand, within HP15_p187g84 at 82.284 kb on - strand, within HP15_p187g84 at 82.302 kb on + strand, within HP15_p187g84 at 82.312 kb on - strand, within HP15_p187g84 at 82.312 kb on - strand, within HP15_p187g84 at 82.329 kb on + strand, within HP15_p187g84 at 82.366 kb on - strand, within HP15_p187g84 at 82.366 kb on - strand, within HP15_p187g84 at 82.374 kb on + strand, within HP15_p187g84 at 82.441 kb on + strand at 82.605 kb on - strand at 82.625 kb on - strand at 82.660 kb on + strand at 82.668 kb on - strand at 82.687 kb on - strand at 82.688 kb on - strand at 82.688 kb on - strand at 82.706 kb on + strand at 82.716 kb on + strand at 82.716 kb on + strand at 82.716 kb on + strand at 82.716 kb on + strand at 82.716 kb on + strand at 82.719 kb on - strand at 82.724 kb on - strand at 82.724 kb on - strand at 82.798 kb on - strand at 82.798 kb on - strand at 82.881 kb on - strand, within HP15_p187g85 at 82.881 kb on - strand, within HP15_p187g85 at 82.903 kb on - strand, within HP15_p187g85 at 82.997 kb on - strand, within HP15_p187g85 at 83.052 kb on - strand, within HP15_p187g85 at 83.067 kb on - strand, within HP15_p187g85 at 83.072 kb on - strand, within HP15_p187g85 at 83.111 kb on + strand, within HP15_p187g85 at 83.116 kb on + strand, within HP15_p187g85 at 83.117 kb on + strand, within HP15_p187g85 at 83.119 kb on - strand, within HP15_p187g85 at 83.124 kb on + strand, within HP15_p187g85 at 83.124 kb on + strand, within HP15_p187g85 at 83.138 kb on + strand, within HP15_p187g85 at 83.231 kb on + strand, within HP15_p187g85 at 83.241 kb on - strand, within HP15_p187g85 at 83.273 kb on - strand, within HP15_p187g85 at 83.276 kb on + strand, within HP15_p187g85 at 83.291 kb on + strand, within HP15_p187g85 at 83.307 kb on + strand, within HP15_p187g85 at 83.319 kb on + strand, within HP15_p187g85 at 83.325 kb on - strand, within HP15_p187g85 at 83.327 kb on - strand, within HP15_p187g85 at 83.327 kb on - strand, within HP15_p187g85 at 83.334 kb on + strand, within HP15_p187g85 at 83.334 kb on + strand, within HP15_p187g85 at 83.334 kb on + strand, within HP15_p187g85 at 83.334 kb on + strand, within HP15_p187g85 at 83.334 kb on + strand, within HP15_p187g85 at 83.337 kb on - strand, within HP15_p187g85 at 83.339 kb on + strand, within HP15_p187g85 at 83.342 kb on - strand, within HP15_p187g85 at 83.342 kb on - strand, within HP15_p187g85 at 83.342 kb on - strand, within HP15_p187g85 at 83.349 kb on + strand, within HP15_p187g85 at 83.355 kb on - strand, within HP15_p187g85 at 83.356 kb on - strand, within HP15_p187g85 at 83.373 kb on + strand, within HP15_p187g85 at 83.373 kb on + strand, within HP15_p187g85 at 83.373 kb on + strand, within HP15_p187g85 at 83.373 kb on - strand, within HP15_p187g85 at 83.381 kb on - strand, within HP15_p187g85 at 83.384 kb on + strand, within HP15_p187g85 at 83.392 kb on - strand, within HP15_p187g85 at 83.392 kb on - strand, within HP15_p187g85 at 83.404 kb on - strand, within HP15_p187g85 at 83.434 kb on + strand, within HP15_p187g85 at 83.446 kb on + strand, within HP15_p187g85 at 83.474 kb on - strand, within HP15_p187g85 at 83.513 kb on + strand, within HP15_p187g85 at 83.513 kb on + strand, within HP15_p187g85 at 83.545 kb on + strand, within HP15_p187g85 at 83.674 kb on + strand, within HP15_p187g85 at 83.682 kb on - strand, within HP15_p187g85 at 83.682 kb on - strand, within HP15_p187g85 at 83.747 kb on + strand at 83.750 kb on - strand at 83.755 kb on - strand at 83.755 kb on - strand at 83.822 kb on + strand at 83.828 kb on + strand at 83.870 kb on - strand at 83.894 kb on + strand at 83.901 kb on + strand at 83.901 kb on + strand at 83.906 kb on + strand at 83.909 kb on - strand at 83.909 kb on - strand at 83.942 kb on + strand at 83.980 kb on + strand at 83.988 kb on - strand at 84.064 kb on + strand, within HP15_p187g86 at 84.100 kb on - strand, within HP15_p187g86 at 84.119 kb on + strand, within HP15_p187g86 at 84.119 kb on + strand, within HP15_p187g86 at 84.119 kb on + strand, within HP15_p187g86 at 84.119 kb on + strand, within HP15_p187g86 at 84.121 kb on + strand, within HP15_p187g86 at 84.124 kb on + strand, within HP15_p187g86 at 84.126 kb on + strand, within HP15_p187g86 at 84.126 kb on + strand, within HP15_p187g86 at 84.126 kb on + strand, within HP15_p187g86 at 84.127 kb on - strand, within HP15_p187g86 at 84.127 kb on - strand, within HP15_p187g86 at 84.127 kb on - strand, within HP15_p187g86 at 84.127 kb on - strand, within HP15_p187g86 at 84.127 kb on - strand, within HP15_p187g86 at 84.134 kb on - strand, within HP15_p187g86 at 84.145 kb on + strand at 84.185 kb on - strand at 84.221 kb on + strand at 84.221 kb on + strand at 84.227 kb on + strand at 84.235 kb on - strand at 84.235 kb on - strand at 84.237 kb on + strand at 84.246 kb on + strand at 84.315 kb on + strand, within HP15_p187g87 at 84.321 kb on + strand, within HP15_p187g87 at 84.321 kb on + strand, within HP15_p187g87 at 84.321 kb on + strand, within HP15_p187g87 at 84.321 kb on + strand, within HP15_p187g87 at 84.329 kb on - strand, within HP15_p187g87 at 84.395 kb on + strand, within HP15_p187g87 at 84.405 kb on + strand, within HP15_p187g87 at 84.408 kb on - strand, within HP15_p187g87 at 84.408 kb on - strand, within HP15_p187g87 at 84.439 kb on - strand, within HP15_p187g87 at 84.496 kb on + strand, within HP15_p187g87 at 84.521 kb on - strand, within HP15_p187g87 at 84.587 kb on + strand, within HP15_p187g87 at 84.640 kb on + strand, within HP15_p187g87 at 84.697 kb on + strand, within HP15_p187g87 at 84.718 kb on - strand, within HP15_p187g87 at 84.781 kb on - strand, within HP15_p187g87 at 84.804 kb on - strand, within HP15_p187g87 at 84.817 kb on - strand, within HP15_p187g87 at 84.829 kb on + strand, within HP15_p187g87
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 81,767 - +0.3 81,779 - -1.8 81,793 + -1.5 81,801 + +2.0 81,801 - -0.1 81,801 - -0.5 81,801 - -4.0 81,801 - +1.8 81,841 + +1.9 81,849 - -1.4 81,859 - -0.1 81,882 + -0.1 81,892 - HP15_p187g84 0.11 -0.1 81,941 - HP15_p187g84 0.19 -1.0 81,951 + HP15_p187g84 0.21 -0.8 81,951 - HP15_p187g84 0.21 -1.3 81,953 - HP15_p187g84 0.21 -2.4 81,958 + HP15_p187g84 0.22 +0.1 81,958 + HP15_p187g84 0.22 +0.2 81,958 + HP15_p187g84 0.22 +0.6 81,966 - HP15_p187g84 0.23 +0.5 81,966 - HP15_p187g84 0.23 +2.0 81,966 - HP15_p187g84 0.23 +1.9 81,970 + HP15_p187g84 0.24 +0.9 81,970 + HP15_p187g84 0.24 -1.3 81,978 - HP15_p187g84 0.25 +0.3 81,991 + HP15_p187g84 0.27 -0.8 81,992 + HP15_p187g84 0.27 -0.2 82,013 + HP15_p187g84 0.30 -1.0 82,013 + HP15_p187g84 0.30 +0.5 82,024 + HP15_p187g84 0.32 +2.5 82,024 + HP15_p187g84 0.32 +1.2 82,024 + HP15_p187g84 0.32 -0.8 82,024 + HP15_p187g84 0.32 -1.5 82,024 + HP15_p187g84 0.32 +1.7 82,031 - HP15_p187g84 0.33 +0.1 82,032 - HP15_p187g84 0.33 +0.3 82,032 - HP15_p187g84 0.33 -0.5 82,064 + HP15_p187g84 0.38 +1.8 82,115 + HP15_p187g84 0.46 +1.9 82,115 + HP15_p187g84 0.46 -1.1 82,116 - HP15_p187g84 0.46 -0.6 82,120 + HP15_p187g84 0.47 -0.4 82,120 + HP15_p187g84 0.47 +1.8 82,123 - HP15_p187g84 0.47 +0.3 82,128 - HP15_p187g84 0.48 -1.0 82,142 - HP15_p187g84 0.50 +0.3 82,153 + HP15_p187g84 0.52 +0.6 82,201 - HP15_p187g84 0.59 +0.4 82,217 - HP15_p187g84 0.62 -1.5 82,262 + HP15_p187g84 0.69 +0.2 82,284 - HP15_p187g84 0.72 +1.6 82,302 + HP15_p187g84 0.75 -0.3 82,312 - HP15_p187g84 0.77 -2.5 82,312 - HP15_p187g84 0.77 -0.1 82,329 + HP15_p187g84 0.79 -0.2 82,366 - HP15_p187g84 0.85 +1.7 82,366 - HP15_p187g84 0.85 -0.4 82,374 + HP15_p187g84 0.86 -0.1 82,441 + -0.3 82,605 - +0.0 82,625 - -0.7 82,660 + -1.4 82,668 - -0.5 82,687 - +1.1 82,688 - -1.1 82,688 - -0.1 82,706 + +0.5 82,716 + -1.1 82,716 + +0.6 82,716 + +2.8 82,716 + -0.9 82,716 + -0.5 82,719 - +1.3 82,724 - +0.3 82,724 - +1.9 82,798 - -1.5 82,798 - +1.4 82,881 - HP15_p187g85 0.11 -0.1 82,881 - HP15_p187g85 0.11 +0.1 82,903 - HP15_p187g85 0.13 -0.7 82,997 - HP15_p187g85 0.22 +0.5 83,052 - HP15_p187g85 0.27 +1.3 83,067 - HP15_p187g85 0.28 +1.0 83,072 - HP15_p187g85 0.29 -0.8 83,111 + HP15_p187g85 0.32 -0.5 83,116 + HP15_p187g85 0.33 +0.1 83,117 + HP15_p187g85 0.33 +0.5 83,119 - HP15_p187g85 0.33 +0.2 83,124 + HP15_p187g85 0.33 -0.2 83,124 + HP15_p187g85 0.33 +0.5 83,138 + HP15_p187g85 0.35 +0.4 83,231 + HP15_p187g85 0.43 -0.7 83,241 - HP15_p187g85 0.44 +1.7 83,273 - HP15_p187g85 0.47 +0.6 83,276 + HP15_p187g85 0.47 -0.6 83,291 + HP15_p187g85 0.49 -1.4 83,307 + HP15_p187g85 0.50 +1.0 83,319 + HP15_p187g85 0.51 -0.4 83,325 - HP15_p187g85 0.52 +0.6 83,327 - HP15_p187g85 0.52 -0.8 83,327 - HP15_p187g85 0.52 -0.5 83,334 + HP15_p187g85 0.53 +2.3 83,334 + HP15_p187g85 0.53 -2.3 83,334 + HP15_p187g85 0.53 +0.2 83,334 + HP15_p187g85 0.53 +1.7 83,334 + HP15_p187g85 0.53 -2.5 83,337 - HP15_p187g85 0.53 -0.4 83,339 + HP15_p187g85 0.53 -0.6 83,342 - HP15_p187g85 0.54 -0.7 83,342 - HP15_p187g85 0.54 +0.0 83,342 - HP15_p187g85 0.54 -1.9 83,349 + HP15_p187g85 0.54 +0.4 83,355 - HP15_p187g85 0.55 +1.9 83,356 - HP15_p187g85 0.55 +2.0 83,373 + HP15_p187g85 0.56 +0.1 83,373 + HP15_p187g85 0.56 -0.4 83,373 + HP15_p187g85 0.56 +0.5 83,373 - HP15_p187g85 0.56 +1.2 83,381 - HP15_p187g85 0.57 -0.9 83,384 + HP15_p187g85 0.57 -2.0 83,392 - HP15_p187g85 0.58 -0.5 83,392 - HP15_p187g85 0.58 -0.7 83,404 - HP15_p187g85 0.59 +0.0 83,434 + HP15_p187g85 0.62 -0.6 83,446 + HP15_p187g85 0.63 +0.1 83,474 - HP15_p187g85 0.66 -1.6 83,513 + HP15_p187g85 0.69 +0.7 83,513 + HP15_p187g85 0.69 -2.1 83,545 + HP15_p187g85 0.72 -2.4 83,674 + HP15_p187g85 0.84 -1.0 83,682 - HP15_p187g85 0.85 +1.8 83,682 - HP15_p187g85 0.85 -0.4 83,747 + -1.5 83,750 - -0.9 83,755 - +1.9 83,755 - +0.2 83,822 + -1.0 83,828 + -2.0 83,870 - -1.2 83,894 + -1.7 83,901 + +0.0 83,901 + -0.9 83,906 + -0.6 83,909 - -0.3 83,909 - -0.7 83,942 + +0.1 83,980 + -0.1 83,988 - -0.0 84,064 + HP15_p187g86 0.33 +0.2 84,100 - HP15_p187g86 0.62 +1.9 84,119 + HP15_p187g86 0.77 -1.0 84,119 + HP15_p187g86 0.77 -2.7 84,119 + HP15_p187g86 0.77 +0.0 84,119 + HP15_p187g86 0.77 +0.6 84,121 + HP15_p187g86 0.79 -3.0 84,124 + HP15_p187g86 0.81 +1.6 84,126 + HP15_p187g86 0.83 -0.4 84,126 + HP15_p187g86 0.83 +0.3 84,126 + HP15_p187g86 0.83 +1.6 84,127 - HP15_p187g86 0.83 -0.5 84,127 - HP15_p187g86 0.83 +0.1 84,127 - HP15_p187g86 0.83 -0.4 84,127 - HP15_p187g86 0.83 +0.4 84,127 - HP15_p187g86 0.83 +1.9 84,134 - HP15_p187g86 0.89 +2.0 84,145 + +0.2 84,185 - +2.0 84,221 + -0.1 84,221 + +1.6 84,227 + +0.3 84,235 - +0.0 84,235 - +0.5 84,237 + +1.2 84,246 + -1.1 84,315 + HP15_p187g87 0.13 -0.3 84,321 + HP15_p187g87 0.13 +0.1 84,321 + HP15_p187g87 0.13 -0.2 84,321 + HP15_p187g87 0.13 +0.3 84,321 + HP15_p187g87 0.13 -1.1 84,329 - HP15_p187g87 0.14 -0.0 84,395 + HP15_p187g87 0.19 -0.1 84,405 + HP15_p187g87 0.20 -0.2 84,408 - HP15_p187g87 0.20 +1.7 84,408 - HP15_p187g87 0.20 +2.3 84,439 - HP15_p187g87 0.23 -1.3 84,496 + HP15_p187g87 0.27 +1.2 84,521 - HP15_p187g87 0.29 -0.5 84,587 + HP15_p187g87 0.34 -2.8 84,640 + HP15_p187g87 0.39 +1.8 84,697 + HP15_p187g87 0.43 -2.6 84,718 - HP15_p187g87 0.45 -0.4 84,781 - HP15_p187g87 0.50 +1.7 84,804 - HP15_p187g87 0.52 -0.5 84,817 - HP15_p187g87 0.53 +0.0 84,829 + HP15_p187g87 0.54 -3.2
Or see this region's nucleotide sequence