Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_p42g19 and HP15_p42g20 overlap by 1 nucleotides HP15_p42g20 and HP15_p42g21 are separated by 348 nucleotides HP15_p42g21 and HP15_p42g22 are separated by 52 nucleotides
HP15_p42g19: HP15_p42g19 - transfer origin protein, TraL, at 12,990 to 13,715
_p42g19
HP15_p42g20: HP15_p42g20 - TraK-like protein of DNA transfer, at 13,715 to 14,146
_p42g20
HP15_p42g21: HP15_p42g21 - oriT binding protein TraJ, at 14,495 to 14,866
_p42g21
HP15_p42g22: HP15_p42g22 - TraI DNA relaxase, at 14,919 to 17,186
_p42g22
Position (kb)
14
15 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 13.540 kb on + strand, within HP15_p42g19 at 13.565 kb on + strand, within HP15_p42g19 at 13.574 kb on + strand, within HP15_p42g19 at 13.623 kb on - strand, within HP15_p42g19 at 13.638 kb on + strand, within HP15_p42g19 at 13.764 kb on + strand, within HP15_p42g20 at 14.067 kb on + strand, within HP15_p42g20 at 14.483 kb on - strand at 14.514 kb on - strand at 14.638 kb on - strand, within HP15_p42g21 at 14.669 kb on - strand, within HP15_p42g21 at 14.671 kb on + strand, within HP15_p42g21 at 14.678 kb on + strand, within HP15_p42g21 at 14.764 kb on - strand, within HP15_p42g21 at 14.963 kb on - strand at 14.986 kb on - strand at 14.997 kb on + strand at 14.997 kb on + strand at 15.000 kb on - strand at 15.074 kb on - strand at 15.091 kb on - strand at 15.168 kb on - strand, within HP15_p42g22 at 15.191 kb on - strand, within HP15_p42g22 at 15.209 kb on + strand, within HP15_p42g22 at 15.217 kb on - strand, within HP15_p42g22 at 15.217 kb on - strand, within HP15_p42g22 at 15.222 kb on - strand, within HP15_p42g22 at 15.224 kb on - strand, within HP15_p42g22 at 15.375 kb on - strand, within HP15_p42g22 at 15.486 kb on + strand, within HP15_p42g22 at 15.491 kb on + strand, within HP15_p42g22 at 15.493 kb on - strand, within HP15_p42g22 at 15.596 kb on - strand, within HP15_p42g22 at 15.601 kb on - strand, within HP15_p42g22 at 15.601 kb on - strand, within HP15_p42g22 at 15.612 kb on + strand, within HP15_p42g22 at 15.615 kb on - strand, within HP15_p42g22 at 15.615 kb on - strand, within HP15_p42g22 at 15.692 kb on - strand, within HP15_p42g22 at 15.724 kb on - strand, within HP15_p42g22 at 15.800 kb on - strand, within HP15_p42g22 at 15.805 kb on - strand, within HP15_p42g22 at 15.851 kb on - strand, within HP15_p42g22
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 13,540 + HP15_p42g19 0.76 -0.3 13,565 + HP15_p42g19 0.79 +1.6 13,574 + HP15_p42g19 0.80 -1.3 13,623 - HP15_p42g19 0.87 -1.0 13,638 + HP15_p42g19 0.89 +0.1 13,764 + HP15_p42g20 0.11 +1.3 14,067 + HP15_p42g20 0.81 -0.7 14,483 - -1.3 14,514 - +1.2 14,638 - HP15_p42g21 0.38 +1.3 14,669 - HP15_p42g21 0.47 -0.8 14,671 + HP15_p42g21 0.47 -0.3 14,678 + HP15_p42g21 0.49 +0.7 14,764 - HP15_p42g21 0.72 +0.0 14,963 - -0.7 14,986 - -0.0 14,997 + -0.2 14,997 + -2.6 15,000 - -1.0 15,074 - -1.8 15,091 - +0.0 15,168 - HP15_p42g22 0.11 +0.1 15,191 - HP15_p42g22 0.12 -0.6 15,209 + HP15_p42g22 0.13 -0.6 15,217 - HP15_p42g22 0.13 +3.1 15,217 - HP15_p42g22 0.13 +1.5 15,222 - HP15_p42g22 0.13 +1.2 15,224 - HP15_p42g22 0.13 +1.1 15,375 - HP15_p42g22 0.20 -0.9 15,486 + HP15_p42g22 0.25 -0.1 15,491 + HP15_p42g22 0.25 +0.1 15,493 - HP15_p42g22 0.25 -1.5 15,596 - HP15_p42g22 0.30 -0.9 15,601 - HP15_p42g22 0.30 +1.2 15,601 - HP15_p42g22 0.30 -0.6 15,612 + HP15_p42g22 0.31 -1.5 15,615 - HP15_p42g22 0.31 -4.3 15,615 - HP15_p42g22 0.31 -0.3 15,692 - HP15_p42g22 0.34 +1.3 15,724 - HP15_p42g22 0.35 +0.3 15,800 - HP15_p42g22 0.39 +0.2 15,805 - HP15_p42g22 0.39 -2.3 15,851 - HP15_p42g22 0.41 -2.2
Or see this region's nucleotide sequence