Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3857 and HP15_3858 are separated by 33 nucleotides HP15_3858 and HP15_3859 overlap by 4 nucleotides HP15_3859 and HP15_3860 are separated by 57 nucleotides HP15_3860 and HP15_3861 are separated by 33 nucleotides
HP15_3857: HP15_3857 - RNA polymerase, sigma 70 subunit, RpoD family, at 4,083,909 to 4,084,907
_3857
HP15_3858: HP15_3858 - aspartate kinase, at 4,084,941 to 4,085,399
_3858
HP15_3859: HP15_3859 - conserved hypothetical protein, at 4,085,396 to 4,085,701
_3859
HP15_3860: HP15_3860 - DNA-3-methyladenine glycosylase I, at 4,085,759 to 4,086,340
_3860
HP15_3861: HP15_3861 - 4'-phosphopantetheinyl transferase, at 4,086,374 to 4,086,928
_3861
Position (kb) 4084
4085
4086 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4083.958 kb on + strand at 4083.985 kb on + strand at 4084.075 kb on + strand, within HP15_3857 at 4084.104 kb on + strand, within HP15_3857 at 4084.276 kb on - strand, within HP15_3857 at 4084.307 kb on + strand, within HP15_3857 at 4084.386 kb on + strand, within HP15_3857 at 4084.460 kb on + strand, within HP15_3857 at 4084.482 kb on + strand, within HP15_3857 at 4084.489 kb on + strand, within HP15_3857 at 4084.515 kb on + strand, within HP15_3857 at 4084.543 kb on + strand, within HP15_3857 at 4084.636 kb on + strand, within HP15_3857 at 4084.676 kb on + strand, within HP15_3857 at 4084.699 kb on + strand, within HP15_3857 at 4084.699 kb on + strand, within HP15_3857 at 4084.770 kb on - strand, within HP15_3857 at 4084.770 kb on - strand, within HP15_3857 at 4084.959 kb on - strand at 4084.959 kb on - strand at 4085.007 kb on + strand, within HP15_3858 at 4085.008 kb on - strand, within HP15_3858 at 4085.083 kb on - strand, within HP15_3858 at 4085.103 kb on - strand, within HP15_3858 at 4085.206 kb on + strand, within HP15_3858 at 4085.274 kb on - strand, within HP15_3858 at 4085.304 kb on + strand, within HP15_3858 at 4085.312 kb on - strand, within HP15_3858 at 4085.357 kb on - strand at 4085.438 kb on - strand, within HP15_3859 at 4085.439 kb on - strand, within HP15_3859 at 4085.472 kb on + strand, within HP15_3859 at 4085.520 kb on - strand, within HP15_3859 at 4085.621 kb on - strand, within HP15_3859 at 4085.674 kb on - strand at 4085.683 kb on + strand at 4085.701 kb on + strand at 4085.711 kb on - strand at 4085.734 kb on - strand at 4085.734 kb on - strand at 4085.794 kb on - strand at 4085.841 kb on - strand, within HP15_3860 at 4085.890 kb on - strand, within HP15_3860 at 4085.890 kb on - strand, within HP15_3860 at 4086.019 kb on + strand, within HP15_3860 at 4086.019 kb on + strand, within HP15_3860 at 4086.066 kb on - strand, within HP15_3860 at 4086.089 kb on + strand, within HP15_3860 at 4086.250 kb on - strand, within HP15_3860 at 4086.266 kb on + strand, within HP15_3860 at 4086.266 kb on + strand, within HP15_3860 at 4086.274 kb on - strand, within HP15_3860 at 4086.306 kb on + strand at 4086.332 kb on - strand at 4086.345 kb on - strand at 4086.356 kb on - strand at 4086.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 4,083,958 + +0.2 4,083,985 + -1.5 4,084,075 + HP15_3857 0.17 -0.2 4,084,104 + HP15_3857 0.20 +0.9 4,084,276 - HP15_3857 0.37 +2.0 4,084,307 + HP15_3857 0.40 +2.1 4,084,386 + HP15_3857 0.48 +2.2 4,084,460 + HP15_3857 0.55 +1.7 4,084,482 + HP15_3857 0.57 -0.3 4,084,489 + HP15_3857 0.58 -0.4 4,084,515 + HP15_3857 0.61 -1.4 4,084,543 + HP15_3857 0.63 +0.2 4,084,636 + HP15_3857 0.73 -0.6 4,084,676 + HP15_3857 0.77 +0.1 4,084,699 + HP15_3857 0.79 -0.3 4,084,699 + HP15_3857 0.79 +2.4 4,084,770 - HP15_3857 0.86 +2.2 4,084,770 - HP15_3857 0.86 +1.9 4,084,959 - -0.8 4,084,959 - -0.7 4,085,007 + HP15_3858 0.14 +1.9 4,085,008 - HP15_3858 0.15 -0.4 4,085,083 - HP15_3858 0.31 +0.2 4,085,103 - HP15_3858 0.35 +2.1 4,085,206 + HP15_3858 0.58 +0.3 4,085,274 - HP15_3858 0.73 -0.7 4,085,304 + HP15_3858 0.79 +1.8 4,085,312 - HP15_3858 0.81 +1.2 4,085,357 - -0.8 4,085,438 - HP15_3859 0.14 +0.3 4,085,439 - HP15_3859 0.14 -2.4 4,085,472 + HP15_3859 0.25 +1.4 4,085,520 - HP15_3859 0.41 -0.2 4,085,621 - HP15_3859 0.74 -0.1 4,085,674 - -0.0 4,085,683 + +0.1 4,085,701 + +2.0 4,085,711 - -0.2 4,085,734 - +1.8 4,085,734 - -1.1 4,085,794 - +1.9 4,085,841 - HP15_3860 0.14 -0.2 4,085,890 - HP15_3860 0.23 -0.0 4,085,890 - HP15_3860 0.23 +0.6 4,086,019 + HP15_3860 0.45 -0.5 4,086,019 + HP15_3860 0.45 +0.4 4,086,066 - HP15_3860 0.53 -0.6 4,086,089 + HP15_3860 0.57 -1.0 4,086,250 - HP15_3860 0.84 +1.2 4,086,266 + HP15_3860 0.87 +1.6 4,086,266 + HP15_3860 0.87 -0.2 4,086,274 - HP15_3860 0.88 +1.6 4,086,306 + -0.3 4,086,332 - +0.5 4,086,345 - -0.9 4,086,356 - -0.9 4,086,366 + +0.3
Or see this region's nucleotide sequence