Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3353 and HP15_3354 are separated by 77 nucleotides HP15_3354 and HP15_3355 are separated by 53 nucleotides HP15_3355 and HP15_3356 overlap by 4 nucleotides
HP15_3353: HP15_3353 - 4-hydroxybenzoate octaprenyltransferase, at 3,514,927 to 3,515,814
_3353
HP15_3354: HP15_3354 - two component transcriptional regulator, winged helix family, at 3,515,892 to 3,516,584
_3354
HP15_3355: HP15_3355 - multi-sensor signal transduction histidine kinase, at 3,516,638 to 3,517,957
_3355
HP15_3356: HP15_3356 - response regulator receiver protein, at 3,517,954 to 3,518,823
_3356
Position (kb)
3516
3517
3518 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3515.827 kb on - strand at 3515.834 kb on + strand at 3515.842 kb on - strand at 3515.880 kb on + strand at 3515.888 kb on + strand at 3516.036 kb on + strand, within HP15_3354 at 3516.131 kb on + strand, within HP15_3354 at 3516.281 kb on - strand, within HP15_3354 at 3516.307 kb on - strand, within HP15_3354 at 3516.319 kb on - strand, within HP15_3354 at 3516.341 kb on + strand, within HP15_3354 at 3516.418 kb on - strand, within HP15_3354 at 3516.609 kb on + strand at 3516.740 kb on - strand at 3516.888 kb on - strand, within HP15_3355 at 3516.930 kb on - strand, within HP15_3355 at 3517.062 kb on + strand, within HP15_3355 at 3517.216 kb on + strand, within HP15_3355 at 3517.294 kb on + strand, within HP15_3355 at 3517.417 kb on - strand, within HP15_3355 at 3517.644 kb on + strand, within HP15_3355 at 3517.743 kb on - strand, within HP15_3355 at 3518.244 kb on - strand, within HP15_3356 at 3518.257 kb on + strand, within HP15_3356 at 3518.271 kb on - strand, within HP15_3356 at 3518.279 kb on + strand, within HP15_3356 at 3518.298 kb on + strand, within HP15_3356 at 3518.315 kb on - strand, within HP15_3356 at 3518.347 kb on - strand, within HP15_3356 at 3518.347 kb on - strand, within HP15_3356 at 3518.357 kb on + strand, within HP15_3356 at 3518.831 kb on + strand at 3518.831 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 3,515,827 - -0.3 3,515,834 + -1.0 3,515,842 - +1.9 3,515,880 + -1.0 3,515,888 + -1.5 3,516,036 + HP15_3354 0.21 +0.3 3,516,131 + HP15_3354 0.34 +0.4 3,516,281 - HP15_3354 0.56 -0.0 3,516,307 - HP15_3354 0.60 -0.3 3,516,319 - HP15_3354 0.62 -2.1 3,516,341 + HP15_3354 0.65 -1.5 3,516,418 - HP15_3354 0.76 -0.6 3,516,609 + -0.7 3,516,740 - +1.7 3,516,888 - HP15_3355 0.19 +3.6 3,516,930 - HP15_3355 0.22 -0.6 3,517,062 + HP15_3355 0.32 +2.9 3,517,216 + HP15_3355 0.44 +0.3 3,517,294 + HP15_3355 0.50 +2.3 3,517,417 - HP15_3355 0.59 +1.5 3,517,644 + HP15_3355 0.76 +2.4 3,517,743 - HP15_3355 0.84 +2.5 3,518,244 - HP15_3356 0.33 +1.6 3,518,257 + HP15_3356 0.35 -1.1 3,518,271 - HP15_3356 0.36 +0.8 3,518,279 + HP15_3356 0.37 -0.2 3,518,298 + HP15_3356 0.40 +1.9 3,518,315 - HP15_3356 0.41 -1.2 3,518,347 - HP15_3356 0.45 -0.9 3,518,347 - HP15_3356 0.45 +1.4 3,518,357 + HP15_3356 0.46 +0.9 3,518,831 + -0.9 3,518,831 + +2.3
Or see this region's nucleotide sequence