Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_209 and HP15_210 are separated by 143 nucleotides HP15_210 and HP15_211 are separated by 11 nucleotides HP15_211 and HP15_212 overlap by 25 nucleotides HP15_212 and HP15_213 are separated by 146 nucleotides
HP15_209: HP15_209 - phosphomannomutase, at 195,696 to 197,054
_209
HP15_210: HP15_210 - phospholipase A(1), at 197,198 to 198,325
_210
HP15_211: HP15_211 - conserved hypothetical protein, membrane, at 198,337 to 198,675
_211
HP15_212: HP15_212 - hypothetical protein, at 198,651 to 198,788
_212
HP15_213: HP15_213 - cytochrome c5, at 198,935 to 199,291
_213
Position (kb) 197
198
199 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 196.482 kb on + strand, within HP15_209 at 196.516 kb on - strand, within HP15_209 at 196.643 kb on - strand, within HP15_209 at 196.743 kb on + strand, within HP15_209 at 196.751 kb on - strand, within HP15_209 at 196.826 kb on + strand, within HP15_209 at 197.002 kb on - strand at 197.121 kb on + strand at 197.155 kb on + strand at 197.172 kb on + strand at 197.215 kb on + strand at 197.215 kb on + strand at 197.223 kb on - strand at 197.234 kb on - strand at 197.297 kb on + strand at 197.307 kb on - strand at 197.411 kb on + strand, within HP15_210 at 197.445 kb on - strand, within HP15_210 at 197.594 kb on - strand, within HP15_210 at 197.594 kb on - strand, within HP15_210 at 197.710 kb on + strand, within HP15_210 at 197.717 kb on + strand, within HP15_210 at 197.725 kb on - strand, within HP15_210 at 197.743 kb on + strand, within HP15_210 at 197.751 kb on - strand, within HP15_210 at 197.800 kb on + strand, within HP15_210 at 197.925 kb on - strand, within HP15_210 at 197.925 kb on - strand, within HP15_210 at 198.011 kb on - strand, within HP15_210 at 198.029 kb on + strand, within HP15_210 at 198.107 kb on - strand, within HP15_210 at 198.123 kb on + strand, within HP15_210 at 198.137 kb on - strand, within HP15_210 at 198.203 kb on + strand, within HP15_210 at 198.222 kb on - strand at 198.359 kb on - strand at 198.403 kb on + strand, within HP15_211 at 198.609 kb on - strand, within HP15_211 at 198.748 kb on - strand, within HP15_212 at 198.813 kb on + strand at 198.821 kb on - strand at 198.885 kb on - strand at 198.909 kb on + strand at 199.022 kb on + strand, within HP15_213 at 199.041 kb on + strand, within HP15_213 at 199.148 kb on + strand, within HP15_213 at 199.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 196,482 + HP15_209 0.58 -1.8 196,516 - HP15_209 0.60 -1.2 196,643 - HP15_209 0.70 +0.5 196,743 + HP15_209 0.77 +1.6 196,751 - HP15_209 0.78 -0.5 196,826 + HP15_209 0.83 -1.0 197,002 - +0.4 197,121 + +1.6 197,155 + +1.7 197,172 + +0.2 197,215 + +0.8 197,215 + +1.6 197,223 - -0.8 197,234 - -1.7 197,297 + +0.2 197,307 - -1.4 197,411 + HP15_210 0.19 -0.4 197,445 - HP15_210 0.22 -3.6 197,594 - HP15_210 0.35 +1.8 197,594 - HP15_210 0.35 +1.5 197,710 + HP15_210 0.45 +0.6 197,717 + HP15_210 0.46 +0.4 197,725 - HP15_210 0.47 -0.2 197,743 + HP15_210 0.48 -1.5 197,751 - HP15_210 0.49 +2.1 197,800 + HP15_210 0.53 -1.1 197,925 - HP15_210 0.64 +1.8 197,925 - HP15_210 0.64 -0.9 198,011 - HP15_210 0.72 -0.6 198,029 + HP15_210 0.74 -1.3 198,107 - HP15_210 0.81 -0.2 198,123 + HP15_210 0.82 +1.1 198,137 - HP15_210 0.83 +2.1 198,203 + HP15_210 0.89 -0.5 198,222 - -0.3 198,359 - +0.9 198,403 + HP15_211 0.19 -0.7 198,609 - HP15_211 0.80 +1.6 198,748 - HP15_212 0.70 -0.9 198,813 + +0.3 198,821 - -1.6 198,885 - -1.5 198,909 + -0.9 199,022 + HP15_213 0.24 +0.8 199,041 + HP15_213 0.30 -0.4 199,148 + HP15_213 0.60 +1.6 199,258 - -1.9
Or see this region's nucleotide sequence