Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_194 and HP15_195 are separated by 260 nucleotides HP15_195 and HP15_196 overlap by 2 nucleotides HP15_196 and HP15_tRNA1 are separated by 549 nucleotides
HP15_194: HP15_194 - mercuric ion reductase, at 182,043 to 183,449
_194
HP15_195: HP15_195 - hypothetical protein, at 183,710 to 183,814
_195
HP15_196: HP15_196 - cytochrome C-like protein, at 183,813 to 184,241
_196
HP15_tRNA1: HP15_tRNA1 - tRNA-Arg, at 184,791 to 184,867
_tRNA1
Position (kb) 183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 182.737 kb on + strand, within HP15_194 at 182.745 kb on - strand, within HP15_194 at 182.760 kb on + strand, within HP15_194 at 182.760 kb on - strand, within HP15_194 at 182.760 kb on - strand, within HP15_194 at 182.879 kb on - strand, within HP15_194 at 182.924 kb on - strand, within HP15_194 at 182.964 kb on + strand, within HP15_194 at 183.082 kb on - strand, within HP15_194 at 183.138 kb on + strand, within HP15_194 at 183.143 kb on + strand, within HP15_194 at 183.151 kb on - strand, within HP15_194 at 183.151 kb on - strand, within HP15_194 at 183.151 kb on - strand, within HP15_194 at 183.151 kb on - strand, within HP15_194 at 183.151 kb on - strand, within HP15_194 at 183.153 kb on - strand, within HP15_194 at 183.157 kb on + strand, within HP15_194 at 183.165 kb on - strand, within HP15_194 at 183.235 kb on - strand, within HP15_194 at 183.245 kb on + strand, within HP15_194 at 183.256 kb on + strand, within HP15_194 at 183.327 kb on + strand at 183.426 kb on - strand at 183.471 kb on - strand at 183.662 kb on + strand at 183.662 kb on + strand at 183.662 kb on + strand at 183.662 kb on + strand at 183.670 kb on - strand at 183.670 kb on - strand at 183.670 kb on - strand at 183.698 kb on + strand at 183.790 kb on - strand, within HP15_195 at 183.792 kb on + strand, within HP15_195 at 184.038 kb on + strand, within HP15_196 at 184.092 kb on - strand, within HP15_196 at 184.246 kb on + strand at 184.256 kb on - strand at 184.394 kb on + strand at 184.394 kb on + strand at 184.402 kb on - strand at 184.425 kb on + strand at 184.432 kb on + strand at 184.448 kb on - strand at 184.613 kb on + strand at 184.673 kb on - strand at 184.695 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 182,737 + HP15_194 0.49 -0.6 182,745 - HP15_194 0.50 +1.8 182,760 + HP15_194 0.51 +2.1 182,760 - HP15_194 0.51 +0.8 182,760 - HP15_194 0.51 +0.1 182,879 - HP15_194 0.59 +1.5 182,924 - HP15_194 0.63 +1.2 182,964 + HP15_194 0.65 +0.2 183,082 - HP15_194 0.74 -0.3 183,138 + HP15_194 0.78 -1.5 183,143 + HP15_194 0.78 -0.2 183,151 - HP15_194 0.79 -0.7 183,151 - HP15_194 0.79 -1.2 183,151 - HP15_194 0.79 +1.0 183,151 - HP15_194 0.79 +2.4 183,151 - HP15_194 0.79 -1.8 183,153 - HP15_194 0.79 +1.1 183,157 + HP15_194 0.79 -0.3 183,165 - HP15_194 0.80 -1.7 183,235 - HP15_194 0.85 +3.8 183,245 + HP15_194 0.85 -0.6 183,256 + HP15_194 0.86 -0.8 183,327 + +0.1 183,426 - +0.2 183,471 - -0.8 183,662 + -0.2 183,662 + +1.8 183,662 + +0.2 183,662 + -1.0 183,670 - +1.3 183,670 - -0.9 183,670 - +0.6 183,698 + +0.1 183,790 - HP15_195 0.76 -1.1 183,792 + HP15_195 0.78 +0.3 184,038 + HP15_196 0.52 +0.8 184,092 - HP15_196 0.65 +1.8 184,246 + -0.6 184,256 - -0.6 184,394 + -0.8 184,394 + -1.6 184,402 - +1.5 184,425 + -1.0 184,432 + +2.1 184,448 - +1.6 184,613 + -1.0 184,673 - -2.8 184,695 - -0.6
Or see this region's nucleotide sequence