Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1534 and HP15_1535 are separated by 73 nucleotides HP15_1535 and HP15_1536 overlap by 4 nucleotides HP15_1536 and HP15_1537 overlap by 2 nucleotides
HP15_1534: HP15_1534 - cell division protein ZipA-like protein, at 1,618,107 to 1,619,120
_1534
HP15_1535: HP15_1535 - NAD-dependent DNA ligase, at 1,619,194 to 1,621,230
_1535
HP15_1536: HP15_1536 - hypothetical protein, at 1,621,227 to 1,621,376
_1536
HP15_1537: HP15_1537 - conserved hypothetical protein, at 1,621,375 to 1,622,631
_1537
Position (kb) 1619
1620
1621
1622 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1618.839 kb on + strand, within HP15_1534 at 1618.893 kb on + strand, within HP15_1534 at 1619.018 kb on + strand, within HP15_1534 at 1621.284 kb on - strand, within HP15_1536 at 1621.542 kb on + strand, within HP15_1537 at 1621.665 kb on - strand, within HP15_1537 at 1621.756 kb on - strand, within HP15_1537 at 1621.825 kb on + strand, within HP15_1537 at 1622.077 kb on + strand, within HP15_1537 at 1622.077 kb on + strand, within HP15_1537 at 1622.156 kb on + strand, within HP15_1537 at 1622.193 kb on + strand, within HP15_1537 at 1622.193 kb on + strand, within HP15_1537 at 1622.193 kb on + strand, within HP15_1537
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 1,618,839 + HP15_1534 0.72 +2.7 1,618,893 + HP15_1534 0.78 +0.5 1,619,018 + HP15_1534 0.90 +1.2 1,621,284 - HP15_1536 0.38 +1.3 1,621,542 + HP15_1537 0.13 +0.0 1,621,665 - HP15_1537 0.23 +1.5 1,621,756 - HP15_1537 0.30 +0.1 1,621,825 + HP15_1537 0.36 -2.6 1,622,077 + HP15_1537 0.56 +1.4 1,622,077 + HP15_1537 0.56 +0.3 1,622,156 + HP15_1537 0.62 -1.6 1,622,193 + HP15_1537 0.65 -0.5 1,622,193 + HP15_1537 0.65 +0.2 1,622,193 + HP15_1537 0.65 +1.9
Or see this region's nucleotide sequence