Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1326 and HP15_1327 are separated by 84 nucleotides HP15_1327 and HP15_1328 are separated by 196 nucleotides HP15_1328 and HP15_1329 are separated by 106 nucleotides
HP15_1326: HP15_1326 - conserved hypothetical protein, at 1,391,042 to 1,392,823
_1326
HP15_1327: HP15_1327 - hypothetical protein, at 1,392,908 to 1,393,261
_1327
HP15_1328: HP15_1328 - nucleoprotein/polynucleotide-associated enzyme, at 1,393,458 to 1,393,994
_1328
HP15_1329: HP15_1329 - DEAD/DEAH box helicase domain protein, at 1,394,101 to 1,395,474
_1329
Position (kb)
1392
1393
1394 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1392.080 kb on + strand, within HP15_1326 at 1392.080 kb on + strand, within HP15_1326 at 1392.217 kb on - strand, within HP15_1326 at 1392.232 kb on - strand, within HP15_1326 at 1392.360 kb on + strand, within HP15_1326 at 1392.381 kb on + strand, within HP15_1326 at 1392.566 kb on + strand, within HP15_1326 at 1392.732 kb on + strand at 1392.806 kb on + strand at 1392.988 kb on + strand, within HP15_1327 at 1392.990 kb on + strand, within HP15_1327 at 1393.061 kb on + strand, within HP15_1327 at 1393.062 kb on - strand, within HP15_1327 at 1393.092 kb on - strand, within HP15_1327 at 1393.180 kb on - strand, within HP15_1327 at 1393.232 kb on - strand at 1393.445 kb on + strand at 1393.453 kb on - strand at 1393.577 kb on + strand, within HP15_1328 at 1393.733 kb on - strand, within HP15_1328 at 1393.845 kb on + strand, within HP15_1328 at 1393.868 kb on - strand, within HP15_1328 at 1394.165 kb on - strand at 1394.195 kb on - strand at 1394.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 1,392,080 + HP15_1326 0.58 +1.9 1,392,080 + HP15_1326 0.58 +0.1 1,392,217 - HP15_1326 0.66 -0.1 1,392,232 - HP15_1326 0.67 -0.1 1,392,360 + HP15_1326 0.74 +0.4 1,392,381 + HP15_1326 0.75 +1.9 1,392,566 + HP15_1326 0.86 +0.5 1,392,732 + +1.4 1,392,806 + -0.5 1,392,988 + HP15_1327 0.23 -0.3 1,392,990 + HP15_1327 0.23 -0.6 1,393,061 + HP15_1327 0.43 +1.1 1,393,062 - HP15_1327 0.44 +0.0 1,393,092 - HP15_1327 0.52 -0.6 1,393,180 - HP15_1327 0.77 +1.1 1,393,232 - -0.4 1,393,445 + -0.3 1,393,453 - -1.5 1,393,577 + HP15_1328 0.22 -2.0 1,393,733 - HP15_1328 0.51 -0.6 1,393,845 + HP15_1328 0.72 -0.7 1,393,868 - HP15_1328 0.76 -0.1 1,394,165 - -1.6 1,394,195 - -1.3 1,394,235 + -0.8
Or see this region's nucleotide sequence