Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3497 and rpoZ are separated by 59 nucleotides rpoZ and Shew_3499 are separated by 13 nucleotides Shew_3499 and Shew_3500 are separated by 34 nucleotides Shew_3500 and Shew_3501 are separated by 68 nucleotides
Shew_3497: Shew_3497 - guanylate kinase (RefSeq), at 4,183,670 to 4,184,293
_3497
Shew_3498: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 4,184,353 to 4,184,634
rpoZ
Shew_3499: Shew_3499 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,184,648 to 4,186,753
_3499
Shew_3500: Shew_3500 - putative endoribonuclease L-PSP (RefSeq), at 4,186,788 to 4,187,171
_3500
Shew_3501: Shew_3501 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 4,187,240 to 4,187,944
_3501
Position (kb) 4184
4185
4186
4187 Strain fitness (log2 ratio) -1
0
1
2
3 at 4184.446 kb on + strand, within rpoZ at 4184.556 kb on + strand, within rpoZ at 4186.169 kb on - strand, within Shew_3499 at 4186.480 kb on + strand, within Shew_3499 at 4186.560 kb on - strand at 4187.011 kb on - strand, within Shew_3500 at 4187.095 kb on - strand, within Shew_3500 at 4187.449 kb on + strand, within Shew_3501
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 4,184,446 + rpoZ Shew_3498 0.33 +1.7 4,184,556 + rpoZ Shew_3498 0.72 +1.2 4,186,169 - Shew_3499 0.72 +3.6 4,186,480 + Shew_3499 0.87 +1.9 4,186,560 - +3.5 4,187,011 - Shew_3500 0.58 +2.6 4,187,095 - Shew_3500 0.80 +2.1 4,187,449 + Shew_3501 0.30 +0.2
Or see this region's nucleotide sequence