Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2487 and Shew_2488 are separated by 51 nucleotides Shew_2488 and ffs are separated by 279 nucleotides ffs and Shew_2489 are separated by 165 nucleotides Shew_2489 and Shew_2490 are separated by 390 nucleotides
Shew_2487: Shew_2487 - histone deacetylase superfamily protein (RefSeq), at 2,926,619 to 2,927,521
_2487
Shew_2488: Shew_2488 - hypothetical protein (RefSeq), at 2,927,573 to 2,927,851
_2488
Shew_R0086: ffs - SRP RNA; RNA component of signal recognition particle (NCBI), at 2,928,131 to 2,928,247
ffs
Shew_2489: Shew_2489 - helix-hairpin-helix repeat-containing competence protein ComEA (RefSeq), at 2,928,413 to 2,928,712
_2489
Shew_2490: Shew_2490 - anion transporter (RefSeq), at 2,929,103 to 2,930,494
_2490
Position (kb)
2928
2929 Strain fitness (log2 ratio)
-2
-1
0
1 at 2928.448 kb on + strand, within Shew_2489 at 2928.456 kb on - strand, within Shew_2489 at 2928.576 kb on - strand, within Shew_2489 at 2928.601 kb on + strand, within Shew_2489 at 2928.619 kb on - strand, within Shew_2489 at 2928.731 kb on + strand at 2928.733 kb on + strand at 2928.739 kb on - strand at 2928.839 kb on - strand at 2928.905 kb on + strand at 2929.076 kb on - strand at 2929.242 kb on - strand at 2929.268 kb on + strand, within Shew_2490 at 2929.316 kb on - strand, within Shew_2490 at 2929.410 kb on + strand, within Shew_2490
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 2,928,448 + Shew_2489 0.12 -0.3 2,928,456 - Shew_2489 0.14 -0.3 2,928,576 - Shew_2489 0.54 +0.0 2,928,601 + Shew_2489 0.63 -1.0 2,928,619 - Shew_2489 0.69 -1.6 2,928,731 + -0.8 2,928,733 + -0.7 2,928,739 - -0.5 2,928,839 - +0.8 2,928,905 + -2.7 2,929,076 - +0.1 2,929,242 - -0.0 2,929,268 + Shew_2490 0.12 +0.9 2,929,316 - Shew_2490 0.15 -0.4 2,929,410 + Shew_2490 0.22 -0.7
Or see this region's nucleotide sequence